Restriction map for
pF1KB9765
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# NO CUT ENZYMES ( 59 enzymes) #
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AatII Acc65I AccIII AflII AgeI
ApaI AscI Asp700I Asp718I BcgI
BclI BfrI BseAI BsiWI BsmBI
BspDI BspEI BspHI BsrDI Bst1107I
BstBI BstXI BstZ17I ClaI CpoI
DraI DrdI EcoRI EcoT22I FbaI
FseI HindIII KpnI MfeI MroI
MunI NruI NsiI NspV PacI
PinAI PmeI PshAI PvuI RcaI
RsrII SacI SalI SapI SbfI
SexAI SfuI SnaBI SpeI Sse8387I
SspI SwaI XbaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AclI 47 AACGTT AA^CGTT
AcsI 418 AAATTT [GA]^AATT[CT]
AocI 1322 CCTCAGG CC^T[ACGT]AGG
Aor51HI 1583 AGCGCT AGC^GCT
ApoI 418 AAATTT [GA]^AATT[CT]
AseI 1156 ATTAAT AT^TAAT
AsnI 1156 ATTAAT AT^TAAT
AviII 852 TGCGCA TGC^GCA
AvrII 453 CCTAGG C^CTAGG
BamHI 30 GGATCC G^GATCC
BbvCI 586 GCTGAGG CCTCAGC(-5/-2)
BciVI 1130 GGATAC GTATCC(6/5)
BlnI 453 CCTAGG C^CTAGG
BsaBI 1566 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1406 ACCGGA [AT]^CCGG[AT]
BseRI 615 CTCCTC GAGGAG(10/8)
BsgI 2029 CTGCAC GTGCAG(16/14)
BsmI 1167 GAATGC GAATGC(1/-1)
BspLU11I 2549 ACATGT A^CATGT
BsrBI 2158 CCGCTC CCGCTC(-3/-3)
BssHII 1679 GCGCGC G^CGCGC
BssSI 841 CTCGTG CACGAG(-5/-1)
Bsu36I 1322 CCTCAGG CC^T[ACGT]AGG
Eco47III 1583 AGCGCT AGC^GCT
Eco81I 1322 CCTCAGG CC^T[ACGT]AGG
EcoNI 570 CCTGTCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 988 GATATC GAT^ATC
FspI 852 TGCGCA TGC^GCA
MamI 1566 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 1424 ACGCGT A^CGCGT
NdeI 901 CATATG CA^TATG
NheI 2584 GCTAGC G^CTAGC
NotI 2080 GCGGCCGC GC^GGCCGC
PaeR7I 914 CTCGAG C^TCGAG
PflMI 828 CCAAATCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 1156 ATTAAT AT^TAAT
Psp1406I 47 AACGTT AA^CGTT
PvuII 535 CAGCTG CAG^CTG
ScaI 1091 AGTACT AGT^ACT
SfiI 2112 GGCCACCGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 1750 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 2188 CCCGGG CCC^GGG
SphI 855 GCATGC GCATG^C
Van91I 828 CCAAATCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 914 CTCGAG C^TCGAG
XmaCI 2188 CCCGGG C^CCGGG
XmaI 2188 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 27 enzymes) #
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AccI 2377 GTCTAC GT^[AC][GT]AC
2659 GTCTAC
AhdI 1297 GACTCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2143 GACCCGCTGTC
AspEI 1297 GACTCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2143 GACCCGCTGTC
AspI 496 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
2047 GACTCGGTC
BbsI 1027 GAAGAC GAAGAC(2/6)
1390 GAAGAC
BglII 1862 AGATCT A^GATCT
1925 AGATCT
BlpI 529 GCTGAGC GC^T[ACGT]AGC
602 GCTCAGC
Bpu1102I 529 GCTGAGC GC^T[ACGT]AGC
602 GCTCAGC
BpuAI 1027 GAAGAC GAAGAC(2/6)
1390 GAAGAC
Bsp1407I 425 TGTACA T^GTACA
2264 TGTACA
BspMI 1607 ACCTGC ACCTGC(4/8)
2438 ACCTGC
BsrGI 425 TGTACA T^GTACA
2264 TGTACA
BstAPI 855 GCATGCCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2289 GCAGAGGTTGC
BstEII 2334 GGTGACC G^GT[ACGT]ACC
2415 GGTGACC
BstPI 2334 GGTGACC G^GT[ACGT]ACC
2415 GGTGACC
CelII 529 GCTGAGC GC^T[ACGT]AGC
602 GCTCAGC
DraIII 2329 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
2530 CACCACGTG
Eam1105I 1297 GACTCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2143 GACCCGCTGTC
EcoO65I 2334 GGTGACC G^GT[ACGT]ACC
2415 GGTGACC
HpaI 2461 GTTAAC GTT^AAC
2476 GTTAAC
NcoI 1235 CCATGG C^CATGG
2513 CCATGG
NspI 855 GCATGC [GA]CATG^[CT]
2549 ACATGT
PflFI 496 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
2047 GACTCGGTC
SspBI 425 TGTACA T^GTACA
2264 TGTACA
StuI 456 AGGCCT AGG^CCT
1685 AGGCCT
TfiI 765 GATTC G^A[AT]TC
1279 GAATC
Tth111I 496 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
2047 GACTCGGTC
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# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI Alw44I AlwI AlwNI ApaLI
AspHI AvaI AvaII BalI BanI
BanII BbeI BbrPI BbvI BcnI
BfaI BglI BmyI BpmI BsaAI
BsaHI BsaI BsaJI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrFI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EagI EarI EclXI
Eco52I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
KasI Ksp632I KspI MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NarI NciI NdeII NgoMIV NlaIII
NlaIV PleI PmaCI PmlI PpuMI
PstI RsaI SacII Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmlI
StyI TaqI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII