Restriction map for
pF1KB7759
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# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcyI AflII AflIII AgeI AhdI
AocI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BamHI BbeI BbrPI BbvCI BcgI
BciVI BclI BfaI BfrI BlnI
BsaAI BsaBI BsaHI BsaI BsiWI
BsmI Bsp1407I BspDI BspHI BspLU11I
BspMI BsrBI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstZ17I Bsu36I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EarI
EclXI Eco52I Eco81I EcoO65I EcoRV
EcoT22I FbaI FseI FspI Hin1I
HindIII HpaI KasI KpnI Ksp632I
KspI MaeI MamI MfeI MluI
MseI MunI MvnI NaeI NarI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI SspI SwaI Tru9I Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AccIII 1241 TCCGGA T^CCGGA
AcsI 1000 GAATTC [GA]^AATT[CT]
Alw44I 1333 GTGCAC G^TGCAC
AlwNI 105 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 755 AGCGCT AGC^GCT
ApaI 17 GGGCCC GGGCC^C
ApaLI 1333 GTGCAC G^TGCAC
ApoI 1000 GAATTC [GA]^AATT[CT]
AspHI 1333 GTGCAC G[AT]GC[AT]^C
AvaI 315 CTCGGG C^[CT]CG[GA]G
BalI 779 TGGCCA TGG^CCA
BanI 720 GGCACC G^G[CT][GA]CC
BglI 401 GCCACAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1322 AGATCT A^GATCT
BsaWI 1241 TCCGGA [AT]^CCGG[AT]
BseAI 1241 TCCGGA T^CCGGA
BsiEI 881 CGATCG CG[GA][CT]^CG
BsiHKAI 1333 GTGCAC G[AT]GC[AT]^C
BsmFI 305 GGGAC GGGAC(10/14)
BsoBI 315 CTCGGG C^[CT]CG[GA]G
BspEI 1241 TCCGGA T^CCGGA
BsrDI 1077 CATTGC GCAATG(2/0)
Eco47III 755 AGCGCT AGC^GCT
EcoNI 117 CCTCAGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1000 GAATTC G^AATTC
FauI 489 GCGGG CCCGC(4/6)
HincII 805 GTTGAC GT[CT]^[GA]AC
HindII 805 GTTGAC GT[CT]^[GA]AC
MluNI 779 TGGCCA TGG^CCA
MroI 1241 TCCGGA T^CCGGA
MscI 779 TGGCCA TGG^CCA
NdeI 1151 CATATG CA^TATG
PvuI 881 CGATCG CGAT^CG
PvuII 108 CAGCTG CAG^CTG
SmlI 946 CTCAAG C^T[CT][GA]AG
StuI 311 AGGCCT AGG^CCT
TfiI 359 GATTC G^A[AT]TC
Tsp45I 703 GTCAC ^GT[GC]AC
Tsp509I 1001 AATT ^AATT
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# TWO CUT ENZYMES ( 23 enzymes) #
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AfaI 155 GTAC GT^AC
915 GTAC
BbsI 870 GAAGAC GAAGAC(2/6)
970 GAAGAC
BlpI 615 GCTCAGC GC^T[ACGT]AGC
840 GCTCAGC
Bpu1102I 615 GCTCAGC GC^T[ACGT]AGC
840 GCTCAGC
BpuAI 870 GAAGAC GAAGAC(2/6)
970 GAAGAC
BseRI 493 GAGGAG GAGGAG(10/8)
547 GAGGAG
BsgI 793 GTGCAG GTGCAG(16/14)
1114 GTGCAG
BsmBI 786 CGTCTC CGTCTC(1/5)
1176 CGTCTC
BsrI 630 ACTGG ACTGG(1/-1)
1230 ACTGG
BstAPI 73 GCACAGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
104 GCAGCAGCTGC
BstXI 141 CCACTCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
269 CCAAAATGCTGG
CelII 615 GCTCAGC GC^T[ACGT]AGC
840 GCTCAGC
DraII 16 GGGGCCC [GA]G^G[ACGT]CC[CT]
17 GGGCCCC
EaeI 411 CGGCCA [CT]^GGCC[GA]
779 TGGCCA
EcoO109I 16 GGGGCCC [GA]G^G[ACGT]CC[CT]
17 GGGCCCC
HaeII 677 GGCGCT [GA]GCGC^[CT]
755 AGCGCT
MaeII 1112 ACGT A^CGT
1175 ACGT
MaeIII 671 GTAAC ^GT[ACGT]AC
703 GTCAC
MspA1I 108 CAGCTG C[AC]G^C[GT]G
1309 CCGCTG
NcoI 422 CCATGG C^CATGG
716 CCATGG
PleI 43 GACTC GAGTC(4/5)
701 GAGTC
RsaI 155 GTAC GT^AC
915 GTAC
SphI 485 GCATGC GCATG^C
1167 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AciI AluI AlwI AvaII BanII
BbvI BcnI BmyI BpmI BsaJI
BsiYI BslI BsmAI Bsp1286I BsrFI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DsaI EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI MboI
MboII MflI MnlI MslI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PstI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TseI TspRI TthHB8I XhoII