Restriction map for pF1KB8905
##################################
# NO CUT ENZYMES (  149 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwI        AocI        Aor51HI     ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspHI       AspI        AviII       
AvrII       BamHI       BbrPI       BbsI        BbvCI       
BciVI       BclI        BfaI        BfrI        BglII       
BlnI        BlpI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaWI       BseAI       BsgI        BsiEI       
BsiHKAI     BsiWI       BsmBI       BsmI        Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrDI       BsrGI       BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstF5I      BstPI       BstYI       
BstZ17I     Bsu36I      CelII       ClaI        CpoI        
DraI        DraIII      DrdI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO65I     EcoRI       EcoRV       EcoT22I     FbaI        
FokI        FseI        FspI        HincII      HindII      
HindIII     HpaI        KpnI        MaeI        MamI        
MfeI        MflI        MluI        MroI        MseI        
MslI        MunI        NaeI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacI        
SalI        SapI        SbfI        ScaI        SexAI       
SfaNI       SfuI        SgrAI       SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        SwaI        
TfiI        Tru9I       Tsp509I     Tth111I     Van91I      
XbaI        XcmI        XhoII       XmnI        


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# ONE CUT ENZYMES (   31 enzymes) #
###################################

AcyI          488  GGCGCC               G[GA]^CG[CT]C
AfaI          216  GTAC                 GT^AC
BalI           23  TGGCCA               TGG^CCA
BbeI          488  GGCGCC               GGCGC^C
BsaHI         488  GGCGCC               G[GA]^CG[CT]C
BsaI          411  GGTCTC               GGTCTC(1/5)
BssHII        570  GCGCGC               G^CGCGC
BstXI          14  CCAGCCCCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DpnII         637  GATC                 ^GATC
Hin1I         488  GGCGCC               G[GA]^CG[CT]C
HinfI         270  GAGTC                G^A[ACGT]TC
HphI          148  TCACC                GGTGA(8/7)
KasI          488  GGCGCC               G^GCGCC
KspI          507  CCGCGG               CCGC^GG
MaeII         374  ACGT                 A^CGT
MaeIII        656  GTGAC                ^GT[ACGT]AC
MboI          637  GATC                 ^GATC
MluNI          23  TGGCCA               TGG^CCA
MscI           23  TGGCCA               TGG^CCA
NarI          488  GGCGCC               GG^CGCC
NdeII         637  GATC                 ^GATC
PaeR7I        669  CTCGAG               C^TCGAG
PleI          270  GAGTC                GAGTC(4/5)
PpuMI         689  GGGACCC              [GA]G^G[AT]CC[CT]
RsaI          216  GTAC                 GT^AC
SacII         507  CCGCGG               CCGC^GG
Sau3AI        637  GATC                 ^GATC
SfoI          488  GGCGCC               GGC^GCC
SmlI          669  CTCGAG               C^T[CT][GA]AG
Tsp45I        656  GTGAC                ^GT[GC]AC
XhoI          669  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AlwNI         238  CAGCAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
              311  CAGTCCCTG           
ApaI          306  GGGCCC               GGGCC^C
              325  GGGCCC              
AvaII         683  GGTCC                G^G[AT]CC
              690  GGACC               
BanI          190  GGCACC               G^G[CT][GA]CC
              488  GGCGCC              
BcgI          416  CGATGTCTGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
              519  GCAGGCCTGTCG        
BpmI          205  CTGGAG               CTGGAG(16/14)
              559  CTGGAG              
BseRI         208  GAGGAG               GAGGAG(10/8)
              355  GAGGAG              
BsmAI         358  GAGAC                GTCTC(1/5)
              412  GTCTC               
BsmFI         689  GGGAC                GGGAC(10/14)
              313  GTCCC               
BsrBI         388  GAGCGG               CCGCTC(-3/-3)
              672  GAGCGG              
BsrFI         437  ACCGGC               [GA]^CCGG[CT]
              659  ACCGGC              
Cfr10I        437  ACCGGC               [GA]^CCGG[CT]
              659  ACCGGC              
EarI          703  CTCTTC               CTCTTC(1/4)
              277  GAAGAG              
HgaI          400  GACGC                GACGC(5/10)
              595  GACGC               
Ksp632I       703  CTCTTC               CTCTTC(1/4)
              277  GAAGAG              
SmaI          626  CCCGGG               CCC^GGG
              679  CCCGGG              
StuI          201  AGGCCT               AGG^CCT
              521  AGGCCT              
TaqI          415  TCGA                 T^CGA
              670  TCGA                
TthHB8I       415  TCGA                 T^CGA
              670  TCGA                
XmaCI         626  CCCGGG               C^CCGGG
              679  CCCGGG              
XmaI          626  CCCGGG               C^CCGGG
              679  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   57 enzymes) #
#########################################

AccII       AciI        AluI        AvaI        BanII       
BbvI        BcnI        BglI        BmyI        BsaJI       
BsiYI       BslI        BsoBI       Bsp1286I    BsrI        
BssKI       BstNI       BstUI       Cac8I       CfoI        
Cfr13I      DdeI        DraII       DsaI        EaeI        
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
HaeII       HaeIII      HapII       HhaI        HinP1I      
HpaII       ItaI        MboII       MnlI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NcoI        NlaIII      NlaIV       PstI        PvuII       
Sau96I      ScrFI       SfcI        SfiI        StyI        
TseI        TspRI       
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