################################## # NO CUT ENZYMES ( 149 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BamHI BbrPI BbsI BbvCI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrGI BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstYI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FokI FseI FspI HincII HindII HindIII HpaI KpnI MaeI MamI MfeI MflI MluI MroI MseI MslI MunI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SalI SapI SbfI ScaI SexAI SfaNI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TfiI Tru9I Tsp509I Tth111I Van91I XbaI XcmI XhoII XmnI ################################### # ONE CUT ENZYMES ( 31 enzymes) # ################################### AcyI 488 GGCGCC G[GA]^CG[CT]C AfaI 216 GTAC GT^AC BalI 23 TGGCCA TGG^CCA BbeI 488 GGCGCC GGCGC^C BsaHI 488 GGCGCC G[GA]^CG[CT]C BsaI 411 GGTCTC GGTCTC(1/5) BssHII 570 GCGCGC G^CGCGC BstXI 14 CCAGCCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DpnII 637 GATC ^GATC Hin1I 488 GGCGCC G[GA]^CG[CT]C HinfI 270 GAGTC G^A[ACGT]TC HphI 148 TCACC GGTGA(8/7) KasI 488 GGCGCC G^GCGCC KspI 507 CCGCGG CCGC^GG MaeII 374 ACGT A^CGT MaeIII 656 GTGAC ^GT[ACGT]AC MboI 637 GATC ^GATC MluNI 23 TGGCCA TGG^CCA MscI 23 TGGCCA TGG^CCA NarI 488 GGCGCC GG^CGCC NdeII 637 GATC ^GATC PaeR7I 669 CTCGAG C^TCGAG PleI 270 GAGTC GAGTC(4/5) PpuMI 689 GGGACCC [GA]G^G[AT]CC[CT] RsaI 216 GTAC GT^AC SacII 507 CCGCGG CCGC^GG Sau3AI 637 GATC ^GATC SfoI 488 GGCGCC GGC^GCC SmlI 669 CTCGAG C^T[CT][GA]AG Tsp45I 656 GTGAC ^GT[GC]AC XhoI 669 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AlwNI 238 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG 311 CAGTCCCTG ApaI 306 GGGCCC GGGCC^C 325 GGGCCC AvaII 683 GGTCC G^G[AT]CC 690 GGACC BanI 190 GGCACC G^G[CT][GA]CC 488 GGCGCC BcgI 416 CGATGTCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 519 GCAGGCCTGTCG BpmI 205 CTGGAG CTGGAG(16/14) 559 CTGGAG BseRI 208 GAGGAG GAGGAG(10/8) 355 GAGGAG BsmAI 358 GAGAC GTCTC(1/5) 412 GTCTC BsmFI 689 GGGAC GGGAC(10/14) 313 GTCCC BsrBI 388 GAGCGG CCGCTC(-3/-3) 672 GAGCGG BsrFI 437 ACCGGC [GA]^CCGG[CT] 659 ACCGGC Cfr10I 437 ACCGGC [GA]^CCGG[CT] 659 ACCGGC EarI 703 CTCTTC CTCTTC(1/4) 277 GAAGAG HgaI 400 GACGC GACGC(5/10) 595 GACGC Ksp632I 703 CTCTTC CTCTTC(1/4) 277 GAAGAG SmaI 626 CCCGGG CCC^GGG 679 CCCGGG StuI 201 AGGCCT AGG^CCT 521 AGGCCT TaqI 415 TCGA T^CGA 670 TCGA TthHB8I 415 TCGA T^CGA 670 TCGA XmaCI 626 CCCGGG C^CCGGG 679 CCCGGG XmaI 626 CCCGGG C^CCGGG 679 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 57 enzymes) # ######################################### AccII AciI AluI AvaI BanII BbvI BcnI BglI BmyI BsaJI BsiYI BslI BsoBI Bsp1286I BsrI BssKI BstNI BstUI Cac8I CfoI Cfr13I DdeI DraII DsaI EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI HaeII HaeIII HapII HhaI HinP1I HpaII ItaI MboII MnlI MspA1I MspI MvaI MvnI MwoI NciI NcoI NlaIII NlaIV PstI PvuII Sau96I ScrFI SfcI SfiI StyI TseI TspRI