Restriction map for
pF1KE0147
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# NO CUT ENZYMES ( 149 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AfaI AflII AflIII
AgeI Alw44I ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspHI AspI AviII AvrII BamHI
BbeI BbvCI BcgI BciVI BclI
BcnI BfaI BfrI BglII BlnI
BsaBI BsaHI BsaI BseAI BseRI
BsiEI BsiHKAI BsiWI BsmAI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstXI BstYI
BstZ17I ClaI CpoI DraI DraIII
DrdI EagI EclXI Eco52I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HgaI Hin1I HincII
HindII HindIII HpaI KasI KpnI
MaeI MamI MfeI MflI MluI
MroI MseI MslI MunI NarI
NciI NcoI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsaI
RsrII SacI SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TaqI TfiI Tru9I Tsp509I
Tth111I TthHB8I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccII 37 CGCG CG^CG
AhdI 140 GACCTCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 303 GGATC GGATC(4/5)
AocI 133 CCTGAGG CC^T[ACGT]AGG
Aor51HI 360 AGCGCT AGC^GCT
AspEI 140 GACCTCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 201 CTCGGG C^[CT]CG[GA]G
BalI 534 TGGCCA TGG^CCA
BanI 337 GGCACC G^G[CT][GA]CC
BanII 445 GAGCCC G[GA]GC[CT]^C
BbrPI 472 CACGTG CAC^GTG
BbsI 481 GTCTTC GAAGAC(2/6)
BglI 347 GCCTGGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 423 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 423 GCTGAGC GC^T[ACGT]AGC
BpuAI 481 GTCTTC GAAGAC(2/6)
BsaAI 472 CACGTG [CT]AC^GT[GA]
BsaWI 144 TCCGGT [AT]^CCGG[AT]
BsoBI 201 CTCGGG C^[CT]CG[GA]G
BspMI 83 ACCTGC ACCTGC(4/8)
BsrBI 75 CCGCTC CCGCTC(-3/-3)
BsrDI 524 GCAATG GCAATG(2/0)
BsrFI 161 GCCGGC [GA]^CCGG[CT]
BstAPI 109 GCAGGCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 37 CGCG CG^CG
Bsu36I 133 CCTGAGG CC^T[ACGT]AGG
CelII 423 GCTGAGC GC^T[ACGT]AGC
CfoI 361 GCGC GCG^C
Cfr10I 161 GCCGGC [GA]^CCGG[CT]
DsaI 36 CCGCGG C^C[GA][CT]GG
EaeI 534 TGGCCA [CT]^GGCC[GA]
Eam1105I 140 GACCTCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 360 AGCGCT AGC^GCT
Eco81I 133 CCTGAGG CC^T[ACGT]AGG
HaeII 360 AGCGCT [GA]GCGC^[CT]
HhaI 361 GCGC GCG^C
HinP1I 361 GCGC G^CGC
HinfI 607 GACTC G^A[ACGT]TC
KspI 36 CCGCGG CCGC^GG
MaeII 473 ACGT A^CGT
MaeIII 510 GTCAC ^GT[ACGT]AC
MluNI 534 TGGCCA TGG^CCA
MscI 534 TGGCCA TGG^CCA
MvnI 37 CGCG CG^CG
NaeI 161 GCCGGC GCC^GGC
NgoMIV 161 GCCGGC G^CCGGC
PflMI 327 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 607 GACTC GAGTC(4/5)
PmaCI 472 CACGTG CAC^GTG
PmlI 472 CACGTG CAC^GTG
SacII 36 CCGCGG CCGC^GG
Tsp45I 510 GTCAC ^GT[GC]AC
Van91I 327 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AlwNI 110 CAGGCACTG CAG[ACGT][ACGT][ACGT]^CTG
328 CAGGCCCTG
BpmI 268 CTGGAG CTGGAG(16/14)
377 CTGGAG
BstF5I 54 GGATG GGATG(2/0)
489 GGATG
DpnII 189 GATC ^GATC
304 GATC
EarI 91 CTCTTC CTCTTC(1/4)
400 CTCTTC
EcoT14I 437 CCAAGG C^C[AT][AT]GG
449 CCTTGG
FauI 158 CCCGC CCCGC(4/6)
556 CCCGC
FokI 54 GGATG GGATG(9/13)
489 GGATG
HapII 145 CCGG C^CGG
162 CCGG
HpaII 145 CCGG C^CGG
162 CCGG
Ksp632I 91 CTCTTC CTCTTC(1/4)
400 CTCTTC
MboI 189 GATC ^GATC
304 GATC
MspI 145 CCGG C^CGG
162 CCGG
NdeII 189 GATC ^GATC
304 GATC
NlaIII 411 CATG CATG^
542 CATG
PpuMI 138 GGGACCT [GA]G^G[AT]CC[CT]
205 GGGACCC
PstI 24 CTGCAG CTGCA^G
30 CTGCAG
PvuII 27 CAGCTG CAG^CTG
374 CAGCTG
Sau3AI 189 GATC ^GATC
304 GATC
SfaNI 3 GATGC GCATC(5/9)
275 GCATC
SfcI 24 CTGCAG C^T[GA][CT]AG
30 CTGCAG
StyI 437 CCAAGG C^C[AT][AT]GG
449 CCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AciI AluI AvaII BbvI BmyI
BsaJI BsgI BsiYI BslI BsmFI
Bsp1286I BsrI BssKI BstNI Cac8I
Cfr13I DdeI DraII EcoO109I EcoRII
Fnu4HI HaeIII HphI ItaI MboII
MnlI MspA1I MvaI MwoI NlaIV
Sau96I ScrFI TseI TspRI