Restriction map for
pF1KE0431
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# NO CUT ENZYMES ( 147 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AgeI AhdI Alw44I AocI Aor51HI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AvaI AviII AvrII
BamHI BbeI BbrPI BbvCI BcgI
BciVI BclI BcnI BglII BlnI
BlpI BpmI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsgI
BsiWI BsmBI BsmFI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstEII BstPI BstYI
Bsu36I CelII Cfr10I ClaI CpoI
DraIII DrdI Eam1105I Eco47III Eco81I
EcoO65I EcoT22I FbaI FseI FspI
HapII HgaI Hin1I HincII HindII
HpaI HpaII KasI KpnI KspI
MamI MfeI MflI MluI MroI
MspA1I MspI MunI NaeI NarI
NciI NdeI NgoMIV NheI NotI
NruI NsiI PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
StuI SwaI Tsp45I TspRI Tth111I
Van91I XbaI XcmI XhoI XhoII
XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccI 456 GTATAC GT^[AC][GT]AC
AflII 843 CTTAAG C^TTAAG
AflIII 313 ACATGT A^C[GA][CT]GT
AlwI 409 GATCC GGATC(4/5)
AlwNI 524 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 5 GGGCCC GGGCC^C
AvaII 18 GGACC G^G[AT]CC
BalI 863 TGGCCA TGG^CCA
BanI 763 GGTGCC G^G[CT][GA]CC
BanII 5 GGGCCC G[GA]GC[CT]^C
BbvI 238 GCTGC GCAGC(8/12)
BfaI 628 CTAG C^TAG
BfrI 843 CTTAAG C^TTAAG
BglI 70 GCCCTGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BseRI 233 GAGGAG GAGGAG(10/8)
BsiEI 26 CGGCCG CG[GA][CT]^CG
BspLU11I 313 ACATGT A^CATGT
BsrI 706 ACTGG ACTGG(1/-1)
BssKI 72 CCTGG ^CC[ACGT]GG
Bst1107I 456 GTATAC GTA^TAC
BstBI 179 TTCGAA TT^CGAA
BstNI 72 CCTGG CC^[AT]GG
BstXI 683 CCAATTTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 456 GTATAC GTA^TAC
DraI 724 TTTAAA TTT^AAA
EagI 26 CGGCCG C^GGCCG
EclXI 26 CGGCCG C^GGCCG
Eco52I 26 CGGCCG C^GGCCG
EcoNI 110 CCTTGAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 160 GAATTC G^AATTC
EcoRII 72 CCTGG ^CC[AT]GG
EcoRV 893 GATATC GAT^ATC
FauI 79 GCGGG CCCGC(4/6)
HaeII 55 AGCGCC [GA]GCGC^[CT]
HindIII 488 AAGCTT A^AGCTT
HphI 107 TCACC GGTGA(8/7)
MaeI 628 CTAG C^TAG
MaeII 533 ACGT A^CGT
MluNI 863 TGGCCA TGG^CCA
MscI 863 TGGCCA TGG^CCA
MvaI 72 CCTGG CC^[AT]GG
NcoI 14 CCATGG C^CATGG
NspI 313 ACATGT [GA]CATG^[CT]
NspV 179 TTCGAA TT^CGAA
ScrFI 72 CCTGG CC^[ACGT]GG
SfcI 422 CTGTAG C^T[GA][CT]AG
SfuI 179 TTCGAA TT^CGAA
TseI 238 GCTGC G^C[AT]GC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcsI 160 GAATTC [GA]^AATT[CT]
558 GAATTT
ApoI 160 GAATTC [GA]^AATT[CT]
558 GAATTT
Asp700I 634 GAAGTTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
733 GAACTAATTC
AspHI 712 GTGCTC G[AT]GC[AT]^C
874 GAGCAC
BbsI 120 GAAGAC GAAGAC(2/6)
132 GAAGAC
BpuAI 120 GAAGAC GAAGAC(2/6)
132 GAAGAC
BsiHKAI 712 GTGCTC G[AT]GC[AT]^C
874 GAGCAC
BsmAI 827 GAGAC GTCTC(1/5)
839 GAGAC
DdeI 95 CTCAG C^T[ACGT]AG
277 CTCAG
DpnII 141 GATC ^GATC
409 GATC
DraII 4 AGGGCCC [GA]G^G[ACGT]CC[CT]
5 GGGCCCC
DsaI 14 CCATGG C^C[GA][CT]GG
29 CCGTGG
EaeI 26 CGGCCG [CT]^GGCC[GA]
863 TGGCCA
EarI 114 GAAGAG CTCTTC(1/4)
427 GAAGAG
EcoO109I 4 AGGGCCC [GA]G^G[ACGT]CC[CT]
5 GGGCCCC
EcoT14I 14 CCATGG C^C[AT][AT]GG
834 CCAAGG
Fnu4HI 25 GCGGC GC^[ACGT]GC
238 GCTGC
ItaI 25 GCGGC GC^[ACGT]GC
238 GCTGC
Ksp632I 114 GAAGAG CTCTTC(1/4)
427 GAAGAG
MaeIII 346 GTTAC ^GT[ACGT]AC
809 GTAAC
MboI 141 GATC ^GATC
409 GATC
NdeII 141 GATC ^GATC
409 GATC
PleI 829 GACTC GAGTC(4/5)
937 GACTC
Sau3AI 141 GATC ^GATC
409 GATC
SmlI 768 CTTGAG C^T[CT][GA]AG
843 CTTAAG
SspI 389 AATATT AAT^ATT
782 AATATT
StyI 14 CCATGG C^C[AT][AT]GG
834 CCAAGG
TaqI 146 TCGA T^CGA
180 TCGA
TthHB8I 146 TCGA T^CGA
180 TCGA
XmnI 634 GAAGTTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
733 GAACTAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AccII AciI AfaI AluI BmyI
BsaJI BsiYI BslI Bsp1286I BstF5I
BstUI Cac8I CfoI Cfr13I FokI
HaeIII HhaI HinP1I HinfI MboII
MnlI MseI MslI MvnI MwoI
NlaIII NlaIV RsaI Sau96I SfaNI
TfiI Tru9I Tsp509I