Restriction map for
pF1KB6839
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# NO CUT ENZYMES ( 176 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AfaI AflII AflIII AgeI
AhdI Alw44I AlwI AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaII AviII AvrII BamHI BbeI
BbrPI BbvCI BciVI BclI BcnI
BfaI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaI
BsaWI BseAI BsiEI BsiWI BsmAI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstYI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DpnII DraII DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO109I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FseI FspI HaeII HapII HhaI
HinP1I HindIII HpaII HphI KasI
KpnI KspI MaeI MamI MboI
MfeI MflI MluI MroI MspA1I
MspI MunI MvnI NaeI NarI
NciI NcoI NdeI NdeII NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshBI Psp1406I PvuI PvuII RcaI
RsaI RsrII SacII SalI SapI
Sau3AI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI SspI StuI
StyI SwaI Tth111I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AcsI 245 GAATTT [GA]^AATT[CT]
AcyI 370 GACGCC G[GA]^CG[CT]C
ApoI 245 GAATTT [GA]^AATT[CT]
AvaI 163 CCCGAG C^[CT]CG[GA]G
BalI 467 TGGCCA TGG^CCA
BanI 454 GGCACC G^G[CT][GA]CC
BcgI 455 GCACCCCAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaHI 370 GACGCC G[GA]^CG[CT]C
BsgI 403 GTGCAG GTGCAG(16/14)
BsoBI 163 CCCGAG C^[CT]CG[GA]G
BssSI 106 CTCGTG CACGAG(-5/-1)
BstF5I 124 CATCC GGATG(2/0)
BstXI 127 CCAGACAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr13I 59 GGGCC G^G[ACGT]CC
DraI 358 TTTAAA TTT^AAA
EaeI 467 TGGCCA [CT]^GGCC[GA]
EarI 345 GAAGAG CTCTTC(1/4)
EcoNI 145 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FokI 124 CATCC GGATG(9/13)
HgaI 370 GACGC GACGC(5/10)
Hin1I 370 GACGCC G[GA]^CG[CT]C
HincII 331 GTTAAC GT[CT]^[GA]AC
HindII 331 GTTAAC GT[CT]^[GA]AC
HinfI 325 GATTC G^A[ACGT]TC
HpaI 331 GTTAAC GTT^AAC
Ksp632I 345 GAAGAG CTCTTC(1/4)
MaeII 277 ACGT A^CGT
MaeIII 298 GTCAC ^GT[ACGT]AC
MluNI 467 TGGCCA TGG^CCA
MscI 467 TGGCCA TGG^CCA
NspI 64 GCATGC [GA]CATG^[CT]
PshAI 276 GACGTATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 385 CTGCAG CTGCA^G
SacI 495 GAGCTC GAGCT^C
Sau96I 59 GGGCC G^G[ACGT]CC
SfcI 385 CTGCAG C^T[GA][CT]AG
SmlI 10 CTCAAG C^T[CT][GA]AG
SphI 64 GCATGC GCATG^C
TfiI 325 GATTC G^A[AT]TC
Tsp45I 298 GTCAC ^GT[GC]AC
Tsp509I 246 AATT ^AATT
TspRI 300 CACTG CA[GC]TG[ACGT][ACGT]^
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# TWO CUT ENZYMES ( 27 enzymes) #
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AluI 8 AGCT AG^CT
496 AGCT
AspHI 289 GTGCTC G[AT]GC[AT]^C
495 GAGCTC
BanII 160 GAGCCC G[GA]GC[CT]^C
495 GAGCTC
BbsI 313 GAAGAC GAAGAC(2/6)
367 GAAGAC
BpmI 146 CTGGAG CTGGAG(16/14)
212 CTGGAG
BpuAI 313 GAAGAC GAAGAC(2/6)
367 GAAGAC
BsaJI 144 CCCTGG C^C[ACGT][ACGT]GG
164 CCGAGG
BsiHKAI 289 GTGCTC G[AT]GC[AT]^C
495 GAGCTC
BsiYI 145 CCTGGAGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
459 CCCAGTCGTGG
BslI 145 CCTGGAGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
459 CCCAGTCGTGG
BsmFI 223 GGGAC GGGAC(10/14)
142 GTCCC
BsrI 317 ACTGG ACTGG(1/-1)
460 CCAGT
BssKI 145 CCTGG ^CC[ACGT]GG
258 CCAGG
BstNI 145 CCTGG CC^[AT]GG
258 CCAGG
DdeI 441 CTCAG C^T[ACGT]AG
491 CTCAG
EcoRII 145 CCTGG ^CC[AT]GG
258 CCAGG
FauI 26 CCCGC CCCGC(4/6)
57 GCGGG
HaeIII 60 GGCC GG^CC
468 GGCC
MseI 332 TTAA T^TAA
359 TTAA
MvaI 145 CCTGG CC^[AT]GG
258 CCAGG
MwoI 80 GCAGCGAGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
113 GCAGTAGTCGC
NlaIII 65 CATG CATG^
156 CATG
ScrFI 145 CCTGG CC^[ACGT]GG
258 CCAGG
SfaNI 409 GCATC GCATC(5/9)
509 GCATC
TaqI 16 TCGA T^CGA
499 TCGA
Tru9I 332 TTAA T^TAA
359 TTAA
TthHB8I 16 TCGA T^CGA
499 TCGA
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# 3 OR MORE CUT ENZYMES ( 13 enzymes) #
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AciI BbvI BmyI BseRI Bsp1286I
Cac8I Fnu4HI ItaI MboII MnlI
MslI NlaIV TseI