################################## # NO CUT ENZYMES ( 168 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AlwI AocI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvrII BalI BamHI BanII BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaI BsaWI BseAI BsiHKAI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BssSI Bst1107I BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I CelII ClaI DdeI DpnII DraI DraII DrdI DsaI Eam1105I EarI Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI HincII HindII HindIII HinfI HpaI HphI KpnI Ksp632I KspI MaeII MaeIII MboI MfeI MflI MluI MluNI MroI MscI MslI MunI NcoI NdeI NdeII NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI SacI SacII SalI SapI Sau3AI SbfI SexAI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI TfiI Tsp45I Tsp509I Tth111I TthHB8I Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### AfaI 300 GTAC GT^AC AlwNI 518 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 402 AGCGCT AGC^GCT BfaI 289 CTAG C^TAG BmyI 192 GGGCAC G[AGT]GC[ACT]^C BsaBI 171 GATGCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsgI 418 GTGCAG GTGCAG(16/14) BsiEI 498 CGGCCG CG[GA][CT]^CG BsmFI 368 GGGAC GGGAC(10/14) Bsp1286I 192 GGGCAC G[AGT]GC[ACT]^C BssHII 477 GCGCGC G^CGCGC BstF5I 45 CATCC GGATG(2/0) CpoI 502 CGGTCCG CG^G[AT]CCG EaeI 498 CGGCCG [CT]^GGCC[GA] EagI 498 CGGCCG C^GGCCG EclXI 498 CGGCCG C^GGCCG Eco47III 402 AGCGCT AGC^GCT Eco52I 498 CGGCCG C^GGCCG FokI 45 CATCC GGATG(9/13) HgaI 526 GCGTC GACGC(5/10) MaeI 289 CTAG C^TAG MamI 171 GATGCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MseI 80 TTAA T^TAA MspA1I 208 CCGCTG C[AC]G^C[GT]G NaeI 132 GCCGGC GCC^GGC NgoMIV 132 GCCGGC G^CCGGC NlaIII 180 CATG CATG^ PstI 406 CTGCAG CTGCA^G RsaI 300 GTAC GT^AC RsrII 502 CGGTCCG CG^G[AT]CCG ScaI 299 AGTACT AGT^ACT SfcI 406 CTGCAG C^T[GA][CT]AG Tru9I 80 TTAA T^TAA XcmI 263 CCATCTTCATCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### AvaII 164 GGACC G^G[AT]CC 503 GGTCC AviII 173 TGCGCA TGC^GCA 455 TGCGCA BcnI 189 CCGGG CC^[GC]GG 496 CCCGG BpmI 295 CTGGAG CTGGAG(16/14) 445 CTGGAG BsaJI 189 CCGGGG C^C[ACGT][ACGT]GG 443 CCCTGG BspMI 51 GCAGGT ACCTGC(4/8) 414 GCAGGT BsrFI 132 GCCGGC [GA]^CCGG[CT] 500 GCCGGT BsrI 149 ACTGG ACTGG(1/-1) 302 ACTGG BstAPI 468 GCAAGAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 517 GCAGTGCCTGC Cfr10I 132 GCCGGC [GA]^CCGG[CT] 500 GCCGGT DraIII 148 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG 514 CACGCAGTG FspI 173 TGCGCA TGC^GCA 455 TGCGCA MboII 107 TCTTC GAAGA(8/7) 266 TCTTC NciI 189 CCGGG CC^[GC]GG 496 CCCGG TspRI 148 CACTG CA[GC]TG[ACGT][ACGT]^ 518 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 41 enzymes) # ######################################### AccII AciI AcyI AluI BanI BbeI BbvI BsaHI BseRI BsiYI BslI BssKI BstNI BstUI Cac8I CfoI Cfr13I EcoRII FauI Fnu4HI HaeII HaeIII HapII HhaI Hin1I HinP1I HpaII ItaI KasI MnlI MspI MvaI MvnI MwoI NarI NlaIV Sau96I ScrFI SfaNI SfoI TseI