Restriction map for
pF1KB8651
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# NO CUT ENZYMES ( 127 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflIII AgeI Alw44I Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp718I AspHI AvrII BamHI BanI
BbeI BbrPI BcgI BciVI BclI
BglII BlnI BsaAI BsaBI BsaWI
BseAI BsiEI BsiHKAI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspLU11I
BsrDI BsrFI BsrGI Bst1107I BstBI
BstEII BstPI BstXI BstYI BstZ17I
Cfr10I ClaI CpoI DraI DrdI
EagI EarI EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI HincII HindII HindIII
HpaI KasI KpnI Ksp632I KspI
MaeII MamI MfeI MflI MluI
MroI MunI NaeI NarI NcoI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfcI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI XbaI XcmI XhoII
XmaCI XmaI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccII 146 CGCG CG^CG
AflII 604 CTTAAG C^TTAAG
AhdI 1198 GACGATGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 846 CCTGAGG CC^T[ACGT]AGG
AspEI 1198 GACGATGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 964 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbsI 1167 GAAGAC GAAGAC(2/6)
BfaI 763 CTAG C^TAG
BfrI 604 CTTAAG C^TTAAG
BglI 244 GCCTGGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1332 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1332 GCTGAGC GC^T[ACGT]AGC
BpuAI 1167 GAAGAC GAAGAC(2/6)
BsaI 388 GAGACC GGTCTC(1/5)
BspHI 122 TCATGA T^CATGA
BspMI 681 GCAGGT ACCTGC(4/8)
BsrBI 139 GAGCGG CCGCTC(-3/-3)
BssHII 145 GCGCGC G^CGCGC
BstAPI 826 GCAGCTGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 146 CGCG CG^CG
Bsu36I 846 CCTGAGG CC^T[ACGT]AGG
CelII 1332 GCTGAGC GC^T[ACGT]AGC
DraIII 177 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 1198 GACGATGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 846 CCTGAGG CC^T[ACGT]AGG
EcoT14I 392 CCAAGG C^C[AT][AT]GG
HaeII 191 GGCGCT [GA]GCGC^[CT]
MaeI 763 CTAG C^TAG
MslI 879 CATGGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 827 CAGCTG C[AC]G^C[GT]G
MvnI 146 CGCG CG^CG
NdeI 11 CATATG CA^TATG
PaeR7I 292 CTCGAG C^TCGAG
PflFI 964 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 842 AGGACCT [GA]G^G[AT]CC[CT]
PvuII 827 CAGCTG CAG^CTG
RcaI 122 TCATGA T^CATGA
SfiI 243 GGCCTGGATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
StyI 392 CCAAGG C^C[AT][AT]GG
Tth111I 964 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 292 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcyI 967 GGCGTC G[GA]^CG[CT]C
1264 GACGCC
AfaI 225 GTAC GT^AC
663 GTAC
AlwI 1452 GATCC GGATC(4/5)
89 GGATC
AlwNI 48 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
1003 CAGTCTCTG
Asp700I 352 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1248 GAATCCATTC
AviII 165 TGCGCA TGC^GCA
1062 TGCGCA
BalI 99 TGGCCA TGG^CCA
881 TGGCCA
BbvCI 933 CCTCAGC CCTCAGC(-5/-2)
400 GCTGAGG
BsaHI 967 GGCGTC G[GA]^CG[CT]C
1264 GACGCC
BsgI 286 CTGCAC GTGCAG(16/14)
111 GTGCAG
BsmAI 388 GAGAC GTCTC(1/5)
1005 GTCTC
BssSI 512 CACGAG CACGAG(-5/-1)
205 CTCGTG
DsaI 233 CCGTGG C^C[GA][CT]GG
1226 CCACGG
FauI 1051 CCCGC CCCGC(4/6)
141 GCGGG
FspI 165 TGCGCA TGC^GCA
1062 TGCGCA
Hin1I 967 GGCGTC G[GA]^CG[CT]C
1264 GACGCC
MluNI 99 TGGCCA TGG^CCA
881 TGGCCA
MscI 99 TGGCCA TGG^CCA
881 TGGCCA
MseI 605 TTAA T^TAA
1034 TTAA
RsaI 225 GTAC GT^AC
663 GTAC
StuI 242 AGGCCT AGG^CCT
302 AGGCCT
Tru9I 605 TTAA T^TAA
1034 TTAA
Tsp509I 566 AATT ^AATT
1438 AATT
XmnI 352 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1248 GAATCCATTC
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# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AciI AluI ApaI AvaI AvaII
BanII BbvI BcnI BmyI BpmI
BsaJI BseRI BsiYI BslI BsmFI
BsoBI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII DraII EaeI EcoO109I EcoRII
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI MaeIII MboI MboII MnlI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PflMI PleI Sau3AI
Sau96I ScrFI SfaNI SmlI TaqI
TfiI TseI Tsp45I TspRI TthHB8I
Van91I