Restriction map for
pF1KB6555
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# NO CUT ENZYMES ( 135 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI ApoI AscI Asp718I
AspEI AviII AvrII BanI BbeI
BbrPI BcgI BciVI BclI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsiEI BsiWI BsmBI BsmI
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrFI BssHII BssSI Bst1107I
BstBI BstZ17I Bsu36I Cac8I CelII
Cfr10I ClaI CpoI DraI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoRI EcoRV EcoT22I
FbaI FseI FspI HaeII HgaI
Hin1I HpaI KasI KpnI MamI
MfeI MluI MroI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
PvuII RcaI RsrII SalI SapI
SbfI ScaI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
Van91I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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Asp700I 390 GAAGCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 994 GAGCTC G[AT]GC[AT]^C
AspI 1042 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 208 CCCGAG C^[CT]CG[GA]G
BamHI 252 GGATCC G^GATCC
BbsI 1039 GAAGAC GAAGAC(2/6)
BfaI 355 CTAG C^TAG
BpuAI 1039 GAAGAC GAAGAC(2/6)
BsgI 928 CTGCAC GTGCAG(16/14)
BsiHKAI 994 GAGCTC G[AT]GC[AT]^C
BsoBI 208 CCCGAG C^[CT]CG[GA]G
Bsp1407I 1082 TGTACA T^GTACA
BsrGI 1082 TGTACA T^GTACA
BstAPI 462 GCAAAAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 435 GGTCACC G^GT[ACGT]ACC
BstF5I 636 CATCC GGATG(2/0)
BstPI 435 GGTCACC G^GT[ACGT]ACC
BstYI 252 GGATCC [GA]^GATC[CT]
CfoI 605 GCGC GCG^C
DraII 103 GGGACCC [GA]G^G[ACGT]CC[CT]
DraIII 263 CACGCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1030 CCGCGG C^C[GA][CT]GG
EarI 1090 CTCTTC CTCTTC(1/4)
EcoO109I 103 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoO65I 435 GGTCACC G^GT[ACGT]ACC
EcoT14I 128 CCAAGG C^C[AT][AT]GG
FokI 636 CATCC GGATG(9/13)
HhaI 605 GCGC GCG^C
HinP1I 605 GCGC G^CGC
HincII 1048 GTCAAC GT[CT]^[GA]AC
HindII 1048 GTCAAC GT[CT]^[GA]AC
Ksp632I 1090 CTCTTC CTCTTC(1/4)
KspI 1030 CCGCGG CCGC^GG
MaeI 355 CTAG C^TAG
MflI 252 GGATCC [GA]^GATC[CT]
MspA1I 1030 CCGCGG C[AC]G^C[GT]G
PflFI 1042 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 617 GACTC GAGTC(4/5)
PpuMI 103 GGGACCC [GA]G^G[AT]CC[CT]
SacI 994 GAGCTC GAGCT^C
SacII 1030 CCGCGG CCGC^GG
SexAI 884 ACCTGGT A^CC[AT]GGT
SspBI 1082 TGTACA T^GTACA
StyI 128 CCAAGG C^C[AT][AT]GG
TspRI 267 CAGTG CA[GC]TG[ACGT][ACGT]^
Tth111I 1042 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 252 GGATCC [GA]^GATC[CT]
XmnI 390 GAAGCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccII 604 CGCG CG^CG
1031 CGCG
AlwI 253 GATCC GGATC(4/5)
252 GGATC
AseI 300 ATTAAT AT^TAAT
313 ATTAAT
AsnI 300 ATTAAT AT^TAAT
313 ATTAAT
BalI 2 TGGCCA TGG^CCA
563 TGGCCA
BanII 901 GGGCTC G[GA]GC[CT]^C
994 GAGCTC
BbvCI 180 CCTCAGC CCTCAGC(-5/-2)
430 GCTGAGG
BbvI 144 GCTGC GCAGC(8/12)
891 GCTGC
BseRI 151 GAGGAG GAGGAG(10/8)
226 GAGGAG
BspMI 173 ACCTGC ACCTGC(4/8)
575 ACCTGC
BstUI 604 CGCG CG^CG
1031 CGCG
BstXI 551 CCAAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
635 CCATCCAAGTGG
DpnII 253 GATC ^GATC
489 GATC
FauI 12 CCCGC CCCGC(4/6)
65 CCCGC
HindIII 277 AAGCTT A^AGCTT
391 AAGCTT
MaeII 738 ACGT A^CGT
1109 ACGT
MboI 253 GATC ^GATC
489 GATC
MluNI 2 TGGCCA TGG^CCA
563 TGGCCA
MscI 2 TGGCCA TGG^CCA
563 TGGCCA
MseI 301 TTAA T^TAA
314 TTAA
MvnI 604 CGCG CG^CG
1031 CGCG
NdeII 253 GATC ^GATC
489 GATC
PshBI 300 ATTAAT AT^TAAT
313 ATTAAT
Sau3AI 253 GATC ^GATC
489 GATC
Tru9I 301 TTAA T^TAA
314 TTAA
TseI 144 GCTGC G^C[AT]GC
891 GCTGC
XcmI 551 CCAAGAGCCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
864 CCAGTTTGCAGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AciI AfaI AluI AvaII BcnI
BmyI BpmI BsaJI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstNI Cfr13I DdeI EaeI EcoRII
Fnu4HI HaeIII HapII HinfI HpaII
HphI ItaI MaeIII MboII MnlI
MslI MspI MvaI MwoI NciI
NlaIII NlaIV PstI RsaI Sau96I
ScrFI SfaNI SfcI TaqI TfiI
Tsp45I Tsp509I TthHB8I