Restriction map for
pF1KE0513
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# NO CUT ENZYMES ( 131 enzymes) #
##################################
Acc65I AccII AflII AgeI Alw44I
AocI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspI AviII
AvrII BamHI BanI BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BfaI BfrI BglI BglII BlnI
BpuAI BsaAI BsaBI BsaI BsiEI
BsiWI BsmAI BsmBI BsmI Bsp1407I
BspDI BspHI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstYI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DraI DrdI EagI EclXI Eco52I
Eco81I EcoO65I EcoT22I FauI FbaI
FseI FspI HindIII HpaI KasI
KpnI KspI MaeI MamI MfeI
MflI MluI MunI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI PvuI RcaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tth111I
XbaI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AatII 586 GACGTC GACGT^C
AccI 411 GTCTAC GT^[AC][GT]AC
AccIII 615 TCCGGA T^CCGGA
AclI 475 AACGTT AA^CGTT
AfaI 18 GTAC GT^AC
AflIII 212 ACATGT A^C[GA][CT]GT
AhdI 403 GACCTGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 158 GGATC GGATC(4/5)
Aor51HI 443 AGCGCT AGC^GCT
AspEI 403 GACCTGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 178 CCCGAG C^[CT]CG[GA]G
BalI 437 TGGCCA TGG^CCA
BcnI 110 CCCGG CC^[GC]GG
BlpI 861 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 861 GCTCAGC GC^T[ACGT]AGC
BseAI 615 TCCGGA T^CCGGA
BsmFI 965 GGGAC GGGAC(10/14)
BsoBI 178 CCCGAG C^[CT]CG[GA]G
BspEI 615 TCCGGA T^CCGGA
BspLU11I 212 ACATGT A^CATGT
BsrI 708 CCAGT ACTGG(1/-1)
BstXI 38 CCAAGGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 861 GCTCAGC GC^T[ACGT]AGC
DpnII 159 GATC ^GATC
DraIII 184 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 978 CCATGG C^C[GA][CT]GG
EaeI 437 TGGCCA [CT]^GGCC[GA]
Eam1105I 403 GACCTGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 170 CTCTTC CTCTTC(1/4)
Eco47III 443 AGCGCT AGC^GCT
EcoNI 201 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 778 GAATTC G^AATTC
EcoRV 886 GATATC GAT^ATC
HgaI 972 GACGC GACGC(5/10)
HincII 658 GTCAAC GT[CT]^[GA]AC
HindII 658 GTCAAC GT[CT]^[GA]AC
HphI 824 TCACC GGTGA(8/7)
Ksp632I 170 CTCTTC CTCTTC(1/4)
MaeIII 823 GTCAC ^GT[ACGT]AC
MboI 159 GATC ^GATC
MluNI 437 TGGCCA TGG^CCA
MroI 615 TCCGGA T^CCGGA
MscI 437 TGGCCA TGG^CCA
MslI 318 CACAGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NciI 110 CCCGG CC^[GC]GG
NcoI 978 CCATGG C^CATGG
NdeII 159 GATC ^GATC
NspI 212 ACATGT [GA]CATG^[CT]
PleI 345 GAGTC GAGTC(4/5)
Psp1406I 475 AACGTT AA^CGTT
RsaI 18 GTAC GT^AC
SacI 67 GAGCTC GAGCT^C
Sau3AI 159 GATC ^GATC
SmlI 262 CTCAAG C^T[CT][GA]AG
TfiI 696 GAATC G^A[AT]TC
Tsp45I 823 GTCAC ^GT[GC]AC
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcsI 124 AAATTT [GA]^AATT[CT]
778 GAATTC
AcyI 586 GACGTC G[GA]^CG[CT]C
972 GACGCC
ApoI 124 AAATTT [GA]^AATT[CT]
778 GAATTC
BsaHI 586 GACGTC G[GA]^CG[CT]C
972 GACGCC
BsaWI 341 ACCGGA [AT]^CCGG[AT]
615 TCCGGA
BspMI 23 ACCTGC ACCTGC(4/8)
95 ACCTGC
BstF5I 228 CATCC GGATG(2/0)
900 GGATG
DdeI 862 CTCAG C^T[ACGT]AG
916 CTGAG
EcoT14I 38 CCAAGG C^C[AT][AT]GG
978 CCATGG
FokI 228 CATCC GGATG(9/13)
900 GGATG
Hin1I 586 GACGTC G[GA]^CG[CT]C
972 GACGCC
HinfI 345 GAGTC G^A[ACGT]TC
696 GAATC
MseI 605 TTAA T^TAA
772 TTAA
NlaIII 213 CATG CATG^
979 CATG
PflMI 246 CCAGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
663 CCAGAAGCTGG
PpuMI 116 AGGACCC [GA]G^G[AT]CC[CT]
401 AGGACCT
PvuII 352 CAGCTG CAG^CTG
864 CAGCTG
SfaNI 901 GATGC GCATC(5/9)
389 GCATC
StyI 38 CCAAGG C^C[AT][AT]GG
978 CCATGG
TaqI 218 TCGA T^CGA
500 TCGA
Tru9I 605 TTAA T^TAA
772 TTAA
TthHB8I 218 TCGA T^CGA
500 TCGA
Van91I 246 CCAGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
663 CCAGAAGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AciI AluI AlwNI AspHI AvaII
BanII BbvI BmyI BpmI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
Bsp1286I BssKI BstNI Cac8I CfoI
Cfr13I DraII EcoO109I EcoRII Fnu4HI
HaeII HaeIII HapII HhaI HinP1I
HpaII ItaI MaeII MboII MnlI
MspA1I MspI MvaI MwoI NlaIV
PstI Sau96I ScrFI SfcI TseI
Tsp509I TspRI XcmI