Restriction map for
pF1KB8215
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# NO CUT ENZYMES ( 181 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcsI AcyI AflII
AflIII AgeI AhdI AocI Aor51HI
ApaI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AviII AvrII BalI BamHI BanI
BbeI BbrPI BbvCI BbvI BcgI
BciVI BclI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsgI
BsiEI BsiWI BsiYI BslI BsmAI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
DsaI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FauI FbaI Fnu4HI FseI FspI
HaeII HgaI Hin1I HindIII HpaI
ItaI KasI KpnI Ksp632I KspI
MaeII MaeIII MamI MfeI MluI
MluNI MroI MscI MspA1I MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
Sse8387I SspBI SspI SwaI TaqI
TseI Tsp45I Tth111I TthHB8I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AfaI 91 GTAC GT^AC
Alw44I 415 GTGCAC G^TGCAC
AlwI 336 GATCC GGATC(4/5)
AlwNI 124 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 415 GTGCAC G^TGCAC
AvaII 273 GGACC G^G[AT]CC
BanII 164 GAGCTC G[GA]GC[CT]^C
BbsI 342 GAAGAC GAAGAC(2/6)
BpuAI 342 GAAGAC GAAGAC(2/6)
BsmFI 272 GGGAC GGGAC(10/14)
BspHI 81 TCATGA T^CATGA
BsrI 123 CCAGT ACTGG(1/-1)
BssSI 350 CTCGTG CACGAG(-5/-1)
BstF5I 183 CATCC GGATG(2/0)
BstNI 160 CCTGG CC^[AT]GG
BstYI 335 AGATCC [GA]^GATC[CT]
CfoI 372 GCGC GCG^C
Cfr13I 273 GGACC G^G[ACGT]CC
DdeI 167 CTCAG C^T[ACGT]AG
DpnII 336 GATC ^GATC
EcoRII 160 CCTGG ^CC[AT]GG
EcoT14I 224 CCAAGG C^C[AT][AT]GG
FokI 183 CATCC GGATG(9/13)
HaeIII 198 GGCC GG^CC
HhaI 372 GCGC GCG^C
HinP1I 372 GCGC G^CGC
HincII 117 GTTGAC GT[CT]^[GA]AC
HindII 117 GTTGAC GT[CT]^[GA]AC
HphI 228 GGTGA GGTGA(8/7)
MboI 336 GATC ^GATC
MflI 335 AGATCC [GA]^GATC[CT]
MseI 179 TTAA T^TAA
MslI 363 CATGCGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI 160 CCTGG CC^[AT]GG
MwoI 247 GCACATAGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeII 336 GATC ^GATC
NlaIV 272 GGGACC GG[ACGT]^[ACGT]CC
NspI 362 GCATGC [GA]CATG^[CT]
PleI 238 GACTC GAGTC(4/5)
PstI 262 CTGCAG CTGCA^G
RcaI 81 TCATGA T^CATGA
RsaI 91 GTAC GT^AC
SacI 164 GAGCTC GAGCT^C
Sau3AI 336 GATC ^GATC
Sau96I 273 GGACC G^G[ACGT]CC
SfcI 262 CTGCAG C^T[GA][CT]AG
SphI 362 GCATGC GCATG^C
StuI 197 AGGCCT AGG^CCT
StyI 224 CCAAGG C^C[AT][AT]GG
Tru9I 179 TTAA T^TAA
Tsp509I 388 AATT ^AATT
TspRI 146 CACTG CA[GC]TG[ACGT][ACGT]^
XhoII 335 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 9 enzymes) #
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BcnI 30 CCCGG CC^[GC]GG
99 CCCGG
BfaI 208 CTAG C^TAG
283 CTAG
BpmI 62 CTCCAG CTGGAG(16/14)
161 CTGGAG
BsaJI 186 CCGAGG C^C[ACGT][ACGT]GG
224 CCAAGG
BseRI 77 CTCCTC GAGGAG(10/8)
188 GAGGAG
Cac8I 45 GCCTGC GC[ACGT]^[ACGT]GC
362 GCATGC
MaeI 208 CTAG C^TAG
283 CTAG
NciI 30 CCCGG CC^[GC]GG
99 CCCGG
SfaNI 244 GATGC GCATC(5/9)
212 GCATC
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# 3 OR MORE CUT ENZYMES ( 15 enzymes) #
#########################################
AluI AspHI BmyI BsiHKAI Bsp1286I
BssKI HapII HinfI HpaII MboII
MnlI MspI NlaIII ScrFI TfiI