Restriction map for
pF1KB6738
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# NO CUT ENZYMES ( 159 enzymes) #
##################################
AatII Acc65I AccI AccIII AcyI
AflII AflIII AhdI Alw44I AlwNI
AocI Aor51HI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BalI BamHI BanI BbeI
BbrPI BbsI BcgI BciVI BfrI
BglI BglII BlnI BlpI BpmI
Bpu1102I BpuAI BsaBI BsaHI BsaI
BseAI BseRI BsgI BsiEI BsiWI
BsmAI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I Bsu36I CelII CfoI ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FauI FseI FspI
HaeII HhaI Hin1I HinP1I HindIII
HpaI KasI KpnI Ksp632I KspI
MamI MfeI MluI MluNI MroI
MscI MseI MslI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PmaCI
PmeI PmlI PshBI PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfaNI SfiI
SfoI SfuI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI TfiI Tru9I Tth111I
Van91I XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AccII 57 CGCG CG^CG
AclI 114 AACGTT AA^CGTT
AcsI 247 GAATTT [GA]^AATT[CT]
AluI 135 AGCT AG^CT
ApaI 284 GGGCCC GGGCC^C
ApoI 247 GAATTT [GA]^AATT[CT]
Asp700I 111 GAAAACGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 273 GAGCAC G[AT]GC[AT]^C
AvaI 106 CCCGGG C^[CT]CG[GA]G
AvaII 44 GGACC G^G[AT]CC
BanII 284 GGGCCC G[GA]GC[CT]^C
BbvCI 136 GCTGAGG CCTCAGC(-5/-2)
BbvI 234 GCTGC GCAGC(8/12)
BclI 368 TGATCA T^GATCA
BfaI 328 CTAG C^TAG
BsaAI 178 TACGTG [CT]AC^GT[GA]
BsiHKAI 273 GAGCAC G[AT]GC[AT]^C
BsoBI 106 CCCGGG C^[CT]CG[GA]G
BsrI 414 CCAGT ACTGG(1/-1)
BstF5I 240 GGATG GGATG(2/0)
BstNI 150 CCTGG CC^[AT]GG
BstUI 57 CGCG CG^CG
BstYI 258 GGATCT [GA]^GATC[CT]
Cac8I 132 GCCAGC GC[ACGT]^[ACGT]GC
DdeI 137 CTGAG C^T[ACGT]AG
DsaI 357 CCACGG C^C[GA][CT]GG
EcoRII 150 CCTGG ^CC[AT]GG
FbaI 368 TGATCA T^GATCA
FokI 240 GGATG GGATG(9/13)
HincII 121 GTCAAC GT[CT]^[GA]AC
HindII 121 GTCAAC GT[CT]^[GA]AC
MaeI 328 CTAG C^TAG
MboII 261 TCTTC GAAGA(8/7)
MflI 258 GGATCT [GA]^GATC[CT]
MspA1I 134 CAGCTG C[AC]G^C[GT]G
MvaI 150 CCTGG CC^[AT]GG
MvnI 57 CGCG CG^CG
MwoI 327 GCTAGTCCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PpuMI 43 GGGACCT [GA]G^G[AT]CC[CT]
PshAI 324 GACGCTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 114 AACGTT AA^CGTT
PstI 235 CTGCAG CTGCA^G
PvuII 134 CAGCTG CAG^CTG
SfcI 235 CTGCAG C^T[GA][CT]AG
SgrAI 276 CACCGGTG C[GA]^CCGG[CT]G
SmaI 106 CCCGGG CCC^GGG
TaqI 23 TCGA T^CGA
TseI 234 GCTGC G^C[AT]GC
Tsp509I 248 AATT ^AATT
TthHB8I 23 TCGA T^CGA
XhoII 258 GGATCT [GA]^GATC[CT]
XmaCI 106 CCCGGG C^CCGGG
XmaI 106 CCCGGG C^CCGGG
XmnI 111 GAAAACGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
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AfaI 266 GTAC GT^AC
417 GTAC
AgeI 164 ACCGGT A^CCGGT
277 ACCGGT
AlwI 219 GATCC GGATC(4/5)
258 GGATC
BmyI 273 GAGCAC G[AGT]GC[ACT]^C
284 GGGCCC
BsaWI 164 ACCGGT [AT]^CCGG[AT]
277 ACCGGT
Bsp1286I 273 GAGCAC G[AGT]GC[ACT]^C
284 GGGCCC
BsrFI 164 ACCGGT [GA]^CCGG[CT]
277 ACCGGT
Cfr10I 164 ACCGGT [GA]^CCGG[CT]
277 ACCGGT
EaeI 2 TGGCCG [CT]^GGCC[GA]
59 CGGCCA
HgaI 55 GACGC GACGC(5/10)
324 GACGC
HinfI 79 GACTC G^A[ACGT]TC
226 GACTC
HphI 216 GGTGA GGTGA(8/7)
308 TCACC
MaeII 115 ACGT A^CGT
179 ACGT
MaeIII 301 GTCAC ^GT[ACGT]AC
307 GTCAC
NlaIII 33 CATG CATG^
255 CATG
PinAI 164 ACCGGT A^CCGGT
277 ACCGGT
PleI 79 GACTC GAGTC(4/5)
226 GACTC
RsaI 266 GTAC GT^AC
417 GTAC
Tsp45I 301 GTCAC ^GT[GC]AC
307 GTCAC
TspRI 230 CACTG CA[GC]TG[ACGT][ACGT]^
303 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 25 enzymes) #
#########################################
AciI BcnI BsaJI BsiYI BslI
BsmFI BssKI Cfr13I DpnII DraII
EcoO109I Fnu4HI HaeIII HapII HpaII
ItaI MboI MnlI MspI NciI
NdeII NlaIV Sau3AI Sau96I ScrFI