Restriction map for
pF1KB7782
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# NO CUT ENZYMES ( 122 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AflIII AhdI Alw44I Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AviII AvrII
BalI BamHI BbrPI BbvCI BcgI
BciVI BclI BfaI BfrI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspHI BspLU11I BsrDI BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I CelII ClaI CpoI DraI
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FseI FspI HpaI KpnI KspI
MaeI MaeII MamI MfeI MluI
MluNI MscI MunI NcoI NdeI
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflMI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI SbfI SfiI SfuI
SnaBI SpeI SphI Sse8387I SspBI
StuI StyI SwaI Van91I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 30 enzymes) #
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AccII 1222 CGCG CG^CG
AccIII 527 TCCGGA T^CCGGA
AgeI 952 ACCGGT A^CCGGT
AocI 607 CCTCAGG CC^T[ACGT]AGG
AspI 1045 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BseAI 527 TCCGGA T^CCGGA
BsiEI 270 CGACCG CG[GA][CT]^CG
BspEI 527 TCCGGA T^CCGGA
BsrBI 1320 GAGCGG CCGCTC(-3/-3)
BstUI 1222 CGCG CG^CG
BstXI 23 CCAAAAACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1154 AGATCC [GA]^GATC[CT]
Bsu36I 607 CCTCAGG CC^T[ACGT]AGG
EaeI 758 CGGCCA [CT]^GGCC[GA]
Eco81I 607 CCTCAGG CC^T[ACGT]AGG
HindIII 472 AAGCTT A^AGCTT
MflI 1154 AGATCC [GA]^GATC[CT]
MroI 527 TCCGGA T^CCGGA
MvnI 1222 CGCG CG^CG
PflFI 1045 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 952 ACCGGT A^CCGGT
SacI 109 GAGCTC GAGCT^C
ScaI 341 AGTACT AGT^ACT
SgrAI 951 CACCGGTG C[GA]^CCGG[CT]G
SmlI 379 CTCAAG C^T[CT][GA]AG
SspI 313 AATATT AAT^ATT
TfiI 581 GAATC G^A[AT]TC
Tsp509I 449 AATT ^AATT
Tth111I 1045 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 1154 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AlwNI 654 CAGCTACTG CAG[ACGT][ACGT][ACGT]^CTG
856 CAGAGGCTG
AspHI 109 GAGCTC G[AT]GC[AT]^C
328 GTGCTC
AvaI 669 CCCGGG C^[CT]CG[GA]G
694 CCCGGG
BbeI 1255 GGCGCC GGCGC^C
1384 GGCGCC
BpmI 127 CTCCAG CTGGAG(16/14)
534 CTCCAG
BsaI 187 GAGACC GGTCTC(1/5)
1018 GAGACC
BsaWI 527 TCCGGA [AT]^CCGG[AT]
952 ACCGGT
BsgI 136 GTGCAG GTGCAG(16/14)
565 GTGCAG
BsiHKAI 109 GAGCTC G[AT]GC[AT]^C
328 GTGCTC
BsoBI 669 CCCGGG C^[CT]CG[GA]G
694 CCCGGG
EarI 1447 GAAGAG CTCTTC(1/4)
1471 GAAGAG
HincII 646 GTCAAC GT[CT]^[GA]AC
1312 GTCAAC
HindII 646 GTCAAC GT[CT]^[GA]AC
1312 GTCAAC
KasI 1255 GGCGCC G^GCGCC
1384 GGCGCC
Ksp632I 1447 GAAGAG CTCTTC(1/4)
1471 GAAGAG
NaeI 277 GCCGGC GCC^GGC
1521 GCCGGC
NarI 1255 GGCGCC GG^CGCC
1384 GGCGCC
NgoMIV 277 GCCGGC G^CCGGC
1521 GCCGGC
PleI 294 GACTC GAGTC(4/5)
47 GAGTC
SexAI 821 ACCTGGT A^CC[AT]GGT
1193 ACCTGGT
SfoI 1255 GGCGCC GGC^GCC
1384 GGCGCC
SmaI 669 CCCGGG CCC^GGG
694 CCCGGG
Tsp45I 115 GTCAC ^GT[GC]AC
778 GTCAC
XcmI 290 CCAGGACTCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1259 CCATCCTCGTGCTGG
XmaCI 669 CCCGGG C^CCGGG
694 CCCGGG
XmaI 669 CCCGGG C^CCGGG
694 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
ApaI AvaII BanI BanII BbsI
BbvI BcnI BglI BmyI BpuAI
BsaHI BsaJI BseRI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrFI
BsrI BssKI BssSI BstF5I BstNI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII EcoO109I EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI MaeIII MboI
MboII MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
TaqI Tru9I TseI TspRI TthHB8I