Restriction map for
pF1KB6340
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# NO CUT ENZYMES ( 120 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaLI ApoI
AscI Asp718I AspEI AspI AviII
AvrII BbeI BbrPI BbsI BciVI
BfrI BglI BglII BlnI BpuAI
BsaAI BsaHI BsaI BsgI BsiWI
BsmAI BsmBI BsmI BspDI BspLU11I
BspMI BsrDI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I Bsu36I
ClaI CpoI DraI DraIII DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FseI FspI HaeII
Hin1I HindIII HpaI KasI KpnI
KspI MfeI MluI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI Psp1406I RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspI
StyI SwaI TspRI Tth111I XhoI
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# ONE CUT ENZYMES ( 62 enzymes) #
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AccII 65 CGCG CG^CG
AccIII 477 TCCGGA T^CCGGA
ApaI 741 GGGCCC GGGCC^C
AseI 834 ATTAAT AT^TAAT
AsnI 834 ATTAAT AT^TAAT
Asp700I 189 GAAGGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 592 CCCGGG C^[CT]CG[GA]G
BalI 842 TGGCCA TGG^CCA
BamHI 557 GGATCC G^GATCC
BanI 861 GGTGCC G^G[CT][GA]CC
BbvCI 148 CCTCAGC CCTCAGC(-5/-2)
BcgI 546 GCAGCGTCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 425 TGATCA T^GATCA
BlpI 30 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 30 GCTAAGC GC^T[ACGT]AGC
BsaBI 426 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 477 TCCGGA T^CCGGA
BsiEI 47 CGATCG CG[GA][CT]^CG
BsoBI 592 CCCGGG C^[CT]CG[GA]G
Bsp1407I 578 TGTACA T^GTACA
BspEI 477 TCCGGA T^CCGGA
BspHI 795 TCATGA T^CATGA
BsrBI 537 CCGCTC CCGCTC(-3/-3)
BsrGI 578 TGTACA T^GTACA
BssSI 421 CTCGTG CACGAG(-5/-1)
BstUI 65 CGCG CG^CG
BstXI 299 CCATGAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 557 GGATCC [GA]^GATC[CT]
CelII 30 GCTAAGC GC^T[ACGT]AGC
DrdI 361 GACGTAGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 822 GAAGAG CTCTTC(1/4)
FbaI 425 TGATCA T^GATCA
HincII 370 GTCAAC GT[CT]^[GA]AC
HindII 370 GTCAAC GT[CT]^[GA]AC
Ksp632I 822 GAAGAG CTCTTC(1/4)
MaeIII 241 GTGAC ^GT[ACGT]AC
MamI 426 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 557 GGATCC [GA]^GATC[CT]
MluNI 842 TGGCCA TGG^CCA
MroI 477 TCCGGA T^CCGGA
MscI 842 TGGCCA TGG^CCA
MseI 835 TTAA T^TAA
MslI 294 CATCCCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 65 CGCG CG^CG
PflMI 561 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 572 GGGACCT [GA]G^G[AT]CC[CT]
PshBI 834 ATTAAT AT^TAAT
PvuI 47 CGATCG CGAT^CG
RcaI 795 TCATGA T^CATGA
SmaI 592 CCCGGG CCC^GGG
SmlI 448 CTTGAG C^T[CT][GA]AG
SspBI 578 TGTACA T^GTACA
StuI 89 AGGCCT AGG^CCT
Tru9I 835 TTAA T^TAA
Tsp45I 241 GTGAC ^GT[GC]AC
Van91I 561 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 762 TCTAGA T^CTAGA
XcmI 144 CCAGCCTCAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 557 GGATCC [GA]^GATC[CT]
XmaCI 592 CCCGGG C^CCGGG
XmaI 592 CCCGGG C^CCGGG
XmnI 189 GAAGGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 27 enzymes) #
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AfaI 350 GTAC GT^AC
579 GTAC
AlwI 558 GATCC GGATC(4/5)
557 GGATC
AspHI 100 GAGCTC G[AT]GC[AT]^C
418 GAGCTC
AvaII 276 GGACC G^G[AT]CC
573 GGACC
BcnI 592 CCCGG CC^[GC]GG
593 CCGGG
BfaI 268 CTAG C^TAG
763 CTAG
BsiHKAI 100 GAGCTC G[AT]GC[AT]^C
418 GAGCTC
BsmFI 21 GGGAC GGGAC(10/14)
572 GGGAC
BsrFI 500 ACCGGC [GA]^CCGG[CT]
858 GCCGGT
CfoI 64 GCGC GCG^C
486 GCGC
Cfr10I 500 ACCGGC [GA]^CCGG[CT]
858 GCCGGT
HgaI 766 GACGC GACGC(5/10)
549 GCGTC
HhaI 64 GCGC GCG^C
486 GCGC
HinP1I 64 GCGC G^CGC
486 GCGC
MaeI 268 CTAG C^TAG
763 CTAG
MaeII 239 ACGT A^CGT
362 ACGT
MboII 495 GAAGA GAAGA(8/7)
822 GAAGA
NciI 592 CCCGG CC^[GC]GG
593 CCGGG
PleI 303 GAGTC GAGTC(4/5)
604 GAGTC
PstI 85 CTGCAG CTGCA^G
781 CTGCAG
PvuII 469 CAGCTG CAG^CTG
751 CAGCTG
RsaI 350 GTAC GT^AC
579 GTAC
SacI 100 GAGCTC GAGCT^C
418 GAGCTC
SfcI 85 CTGCAG C^T[GA][CT]AG
781 CTGCAG
TaqI 94 TCGA T^CGA
133 TCGA
Tsp509I 669 AATT ^AATT
833 AATT
TthHB8I 94 TCGA T^CGA
133 TCGA
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# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AciI AluI AlwNI BanII BbvI
BmyI BpmI BsaJI BsaWI BseRI
BsiYI BslI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII DraII EaeI EcoO109I EcoRII
FauI Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI MboI
MnlI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV Sau3AI Sau96I
ScrFI SfaNI TfiI TseI