Restriction map for
pF1KE0536
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# NO CUT ENZYMES ( 171 enzymes) #
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AatII Acc65I AccII AccIII AciI
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AviII AvrII BalI BamHI BanI
BbeI BbrPI BbvCI BcgI BciVI
BcnI BfrI BglI BglII BlnI
BlpI BpmI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BseRI
BsgI BsiEI BsiHKAI BsiWI BsmBI
BspEI BspLU11I BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI
BstUI Bsu36I CelII CfoI Cfr10I
CpoI DraI DraIII DrdI DsaI
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoRV
EcoT22I FauI FseI FspI HaeII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HpaI HpaII
KasI KpnI Ksp632I KspI MamI
MluI MluNI MroI MscI MslI
MspA1I MspI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfaNI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI TfiI TspRI Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccI 168 GTATAC GT^[AC][GT]AC
AvaI 229 CCCGAG C^[CT]CG[GA]G
AvaII 520 GGACC G^G[AT]CC
BanII 234 GGGCTC G[GA]GC[CT]^C
BclI 8 TGATCA T^GATCA
BmyI 234 GGGCTC G[AGT]GC[ACT]^C
BsiYI 349 CCAGACTTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 349 CCAGACTTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsoBI 229 CCCGAG C^[CT]CG[GA]G
Bsp1286I 234 GGGCTC G[AGT]GC[ACT]^C
Bsp1407I 372 TGTACA T^GTACA
BspDI 706 ATCGAT AT^CGAT
BspHI 249 TCATGA T^CATGA
BsrGI 372 TGTACA T^GTACA
BssKI 260 CCTGG ^CC[ACGT]GG
Bst1107I 168 GTATAC GTA^TAC
BstEII 585 GGTGACC G^GT[ACGT]ACC
BstNI 260 CCTGG CC^[AT]GG
BstPI 585 GGTGACC G^GT[ACGT]ACC
BstXI 388 CCAAACACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 384 AGATCC [GA]^GATC[CT]
BstZ17I 168 GTATAC GTA^TAC
ClaI 706 ATCGAT AT^CGAT
DdeI 749 CTCAG C^T[ACGT]AG
EcoO65I 585 GGTGACC G^GT[ACGT]ACC
EcoRI 16 GAATTC G^AATTC
EcoRII 260 CCTGG ^CC[AT]GG
EcoT14I 239 CCTTGG C^C[AT][AT]GG
FbaI 8 TGATCA T^GATCA
HaeIII 511 GGCC GG^CC
HinfI 747 GACTC G^A[ACGT]TC
MaeII 796 ACGT A^CGT
MfeI 287 CAATTG C^AATTG
MflI 384 AGATCC [GA]^GATC[CT]
MunI 287 CAATTG C^AATTG
MvaI 260 CCTGG CC^[AT]GG
MwoI 259 GCCTGGAATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PleI 747 GACTC GAGTC(4/5)
PpuMI 519 GGGACCC [GA]G^G[AT]CC[CT]
RcaI 249 TCATGA T^CATGA
ScrFI 260 CCTGG CC^[ACGT]GG
SfcI 279 CTGTAG C^T[GA][CT]AG
SspBI 372 TGTACA T^GTACA
StyI 239 CCTTGG C^C[AT][AT]GG
Tsp45I 586 GTGAC ^GT[GC]AC
XhoII 384 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 22 enzymes) #
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AfaI 270 GTAC GT^AC
373 GTAC
AlwI 385 GATCC GGATC(4/5)
704 GGATC
BbvI 63 GCAGC GCAGC(8/12)
802 GCTGC
BfaI 86 CTAG C^TAG
760 CTAG
BsaJI 230 CCGAGG C^C[ACGT][ACGT]GG
239 CCTTGG
BsmAI 97 GAGAC GTCTC(1/5)
745 GAGAC
BsmI 264 GAATGC GAATGC(1/-1)
208 GCATTC
BstF5I 150 GGATG GGATG(2/0)
218 GGATG
Cfr13I 511 GGCCC G^G[ACGT]CC
520 GGACC
DraII 510 AGGCCCT [GA]G^G[ACGT]CC[CT]
519 GGGACCC
EcoO109I 510 AGGCCCT [GA]G^G[ACGT]CC[CT]
519 GGGACCC
Fnu4HI 63 GCAGC GC^[ACGT]GC
802 GCTGC
FokI 150 GGATG GGATG(9/13)
218 GGATG
HphI 585 GGTGA GGTGA(8/7)
186 TCACC
ItaI 63 GCAGC GC^[ACGT]GC
802 GCTGC
MaeI 86 CTAG C^TAG
760 CTAG
MaeIII 292 GTAAC ^GT[ACGT]AC
586 GTGAC
MseI 127 TTAA T^TAA
326 TTAA
RsaI 270 GTAC GT^AC
373 GTAC
Sau96I 511 GGCCC G^G[ACGT]CC
520 GGACC
Tru9I 127 TTAA T^TAA
326 TTAA
TseI 63 GCAGC G^C[AT]GC
802 GCTGC
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# 3 OR MORE CUT ENZYMES ( 19 enzymes) #
#########################################
AcsI AluI ApoI BbsI BpuAI
BsmFI BsrI Cac8I DpnII MboI
MboII MnlI NdeII NlaIII NlaIV
Sau3AI TaqI Tsp509I TthHB8I