Restriction map for
pF1KE0351
##################################
# NO CUT ENZYMES ( 97 enzymes) #
##################################
AatII AclI AcsI AflII AflIII
AgeI AhdI Aor51HI ApoI AscI
AseI AsnI AspEI AviII AvrII
BbrPI BbvCI BciVI BclI BfrI
BglII BlnI BsiWI BsmBI Bsp1407I
BspDI BspHI BspLU11I BsrFI BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstXI BstZ17I Cfr10I ClaI CpoI
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HindIII HpaI KspI MfeI MluI
MunI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RcaI RsrII SacII ScaI SexAI
SfiI SfuI SgrAI SnaBI SpeI
SphI SspBI SspI SwaI TfiI
Tsp509I XhoI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AccI 153 GTCGAC GT^[AC][GT]AC
AccIII 359 TCCGGA T^CCGGA
Alw44I 838 GTGCAC G^TGCAC
ApaI 2072 GGGCCC GGGCC^C
ApaLI 838 GTGCAC G^TGCAC
Asp700I 363 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 286 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1541 GGATCC G^GATCC
BbeI 662 GGCGCC GGCGC^C
BbsI 2326 GAAGAC GAAGAC(2/6)
BcgI 2174 CGATCCTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 726 GCCCCTGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 2326 GAAGAC GAAGAC(2/6)
BsaBI 493 GATGTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 938 GAGACC GGTCTC(1/5)
BseAI 359 TCCGGA T^CCGGA
BsiEI 155 CGACCG CG[GA][CT]^CG
BsmI 2134 GAATGC GAATGC(1/-1)
BspEI 359 TCCGGA T^CCGGA
BsrBI 657 GAGCGG CCGCTC(-3/-3)
BsrDI 683 GCAATG GCAATG(2/0)
BssSI 1728 CTCGTG CACGAG(-5/-1)
BstAPI 918 GCAGGCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraI 1765 TTTAAA TTT^AAA
HincII 153 GTCGAC GT[CT]^[GA]AC
HindII 153 GTCGAC GT[CT]^[GA]AC
KasI 662 GGCGCC G^GCGCC
MamI 493 GATGTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 359 TCCGGA T^CCGGA
MslI 519 CACCGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 662 GGCGCC GG^CGCC
PflFI 286 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 196 GACACCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SalI 153 GTCGAC G^TCGAC
SapI 1500 GCTCTTC GCTCTTC(1/4)
SfoI 662 GGCGCC GGC^GCC
Tth111I 286 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2424 TCTAGA T^CTAGA
XmnI 363 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
Acc65I 713 GGTACC G^GTACC
957 GGTACC
AcyI 662 GGCGCC G[GA]^CG[CT]C
1939 GACGCC
Asp718I 713 GGTACC G^GTACC
957 GGTACC
BsaAI 136 TACGTG [CT]AC^GT[GA]
1089 TACGTG
BsaHI 662 GGCGCC G[GA]^CG[CT]C
1939 GACGCC
BsaWI 359 TCCGGA [AT]^CCGG[AT]
2417 ACCGGA
EarI 1501 CTCTTC CTCTTC(1/4)
2143 CTCTTC
EcoNI 912 CCTGAAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
942 CCTGCAGGAGG
HgaI 186 GACGC GACGC(5/10)
1939 GACGC
Hin1I 662 GGCGCC G[GA]^CG[CT]C
1939 GACGCC
KpnI 713 GGTACC GGTAC^C
957 GGTACC
Ksp632I 1501 CTCTTC CTCTTC(1/4)
2143 CTCTTC
MseI 176 TTAA T^TAA
1766 TTAA
SbfI 942 CCTGCAGG CCTGCA^GG
2388 CCTGCAGG
SmlI 805 CTCAAG C^T[CT][GA]AG
1386 CTTGAG
Sse8387I 942 CCTGCAGG CCTGCA^GG
2388 CCTGCAGG
Tru9I 176 TTAA T^TAA
1766 TTAA
XcmI 1178 CCAAGAGCAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1262 CCACGACCTTTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AocI AspHI AvaI AvaII
BalI BanI BanII BbvI BcnI
BfaI BlpI BmyI BpmI Bpu1102I
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstUI BstYI Bsu36I Cac8I CelII
CfoI Cfr13I DdeI DpnII DraII
EaeI Eco81I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PflMI PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI StuI StyI
TaqI TseI Tsp45I TspRI TthHB8I
Van91I XhoII XmaCI XmaI