Restriction map for
pF1KB7581
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# NO CUT ENZYMES ( 181 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AfaI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AvaII AviII
AvrII BalI BanI BbeI BbrPI
BbvCI BcgI BciVI BclI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaWI BseAI
BsiEI BsiHKAI BsiWI BsmBI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstF5I BstPI BstUI BstXI BstZ17I
Bsu36I CelII Cfr13I ClaI CpoI
DraI DraII DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FokI FseI
FspI HgaI Hin1I HincII HindII
HindIII HpaI KasI KpnI KspI
MaeII MaeIII MamI MfeI MluI
MluNI MroI MscI MseI MunI
MvnI NarI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsaI RsrII SacI SacII SalI
SapI Sau96I SbfI ScaI SexAI
SfaNI SfcI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tru9I Tsp45I Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 31 enzymes) #
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BamHI 496 GGATCC G^GATCC
BbsI 63 GTCTTC GAAGAC(2/6)
BpmI 503 CTGGAG CTGGAG(16/14)
BpuAI 63 GTCTTC GAAGAC(2/6)
BsaI 445 GAGACC GGTCTC(1/5)
BsmAI 445 GAGAC GTCTC(1/5)
BsmFI 485 GTCCC GGGAC(10/14)
BsmI 315 GAATGC GAATGC(1/-1)
BsrFI 154 GCCGGC [GA]^CCGG[CT]
BstYI 496 GGATCC [GA]^GATC[CT]
Cfr10I 154 GCCGGC [GA]^CCGG[CT]
DdeI 245 CTCAG C^T[ACGT]AG
DpnII 497 GATC ^GATC
DsaI 119 CCATGG C^C[GA][CT]GG
EaeI 2 TGGCCG [CT]^GGCC[GA]
EarI 442 GAAGAG CTCTTC(1/4)
HaeIII 3 GGCC GG^CC
HphI 102 GGTGA GGTGA(8/7)
Ksp632I 442 GAAGAG CTCTTC(1/4)
MboI 497 GATC ^GATC
MflI 496 GGATCC [GA]^GATC[CT]
MslI 117 CACCATGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 154 GCCGGC GCC^GGC
NcoI 119 CCATGG C^CATGG
NdeII 497 GATC ^GATC
NgoMIV 154 GCCGGC G^CCGGC
PleI 389 GAGTC GAGTC(4/5)
Sau3AI 497 GATC ^GATC
Tsp509I 330 AATT ^AATT
TspRI 174 CACTG CA[GC]TG[ACGT][ACGT]^
XhoII 496 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AlwI 497 GATCC GGATC(4/5)
496 GGATC
AlwNI 30 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG
139 CAGAAGCTG
BanII 477 GAGCCC G[GA]GC[CT]^C
506 GAGCCC
BfaI 25 CTAG C^TAG
301 CTAG
BmyI 477 GAGCCC G[AGT]GC[ACT]^C
506 GAGCCC
BseRI 109 CTCCTC GAGGAG(10/8)
289 GAGGAG
BsgI 384 GTGCAG GTGCAG(16/14)
402 GTGCAG
BsiYI 92 CCATTTCGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
487 CCCCTACCCGG
BslI 92 CCATTTCGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
487 CCCCTACCCGG
Bsp1286I 477 GAGCCC G[AGT]GC[ACT]^C
506 GAGCCC
BsrI 198 CCAGT ACTGG(1/-1)
482 CCAGT
CfoI 17 GCGC GCG^C
454 GCGC
EcoT14I 119 CCATGG C^C[AT][AT]GG
320 CCTTGG
HaeII 16 AGCGCC [GA]GCGC^[CT]
453 GGCGCT
HhaI 17 GCGC GCG^C
454 GCGC
HinP1I 17 GCGC G^CGC
454 GCGC
MaeI 25 CTAG C^TAG
301 CTAG
MspA1I 30 CAGCGG C[AC]G^C[GT]G
379 CAGCGG
NlaIII 52 CATG CATG^
120 CATG
StyI 119 CCATGG C^C[AT][AT]GG
320 CCTTGG
TaqI 97 TCGA T^CGA
113 TCGA
TthHB8I 97 TCGA T^CGA
113 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
#########################################
AciI AluI BbvI BcnI BsaJI
BssKI BstNI Cac8I EcoRII Fnu4HI
HapII HinfI HpaII ItaI MboII
MnlI MspI MvaI MwoI NciI
NlaIV ScrFI TfiI TseI