Restriction map for
pF1KB6157
##################################
# NO CUT ENZYMES ( 113 enzymes) #
##################################
AatII Acc65I AccII AclI AflIII
AgeI Aor51HI ApaI AscI AseI
AsnI Asp700I Asp718I AspI AviII
AvrII BamHI BbrPI BcgI BciVI
BclI BlnI BpmI BsaBI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspHI BspLU11I BsrBI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstUI
BstXI BstZ17I ClaI CpoI DraI
DraII DraIII DrdI DsaI EagI
EclXI Eco47III Eco52I EcoNI EcoO109I
EcoRI EcoRV EcoT14I FbaI FseI
FspI HgaI HpaI KpnI KspI
MamI MfeI MluI MunI MvnI
NcoI NdeI NotI NruI NspI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
StuI StyI SwaI Tth111I XbaI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AccIII 1465 TCCGGA T^CCGGA
AcsI 848 AAATTC [GA]^AATT[CT]
AcyI 1679 GGCGCC G[GA]^CG[CT]C
AflII 871 CTTAAG C^TTAAG
AhdI 1561 GACTATGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1285 GTGCAC G^TGCAC
AlwNI 16 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1725 CCTTAGG CC^T[ACGT]AGG
ApaLI 1285 GTGCAC G^TGCAC
ApoI 848 AAATTC [GA]^AATT[CT]
AspEI 1561 GACTATGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 1679 GGCGCC GGCGC^C
BbvCI 1629 GCTGAGG CCTCAGC(-5/-2)
BcnI 825 CCGGG CC^[GC]GG
BfrI 871 CTTAAG C^TTAAG
BglI 764 GCCAGATTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 1065 CACGTA [CT]AC^GT[GA]
BsaHI 1679 GGCGCC G[GA]^CG[CT]C
BsaI 821 GAGACC GGTCTC(1/5)
BsaWI 1465 TCCGGA [AT]^CCGG[AT]
BseAI 1465 TCCGGA T^CCGGA
BsmFI 1267 GTCCC GGGAC(10/14)
BspEI 1465 TCCGGA T^CCGGA
BsrDI 1770 CATTGC GCAATG(2/0)
BstEII 510 GGTTACC G^GT[ACGT]ACC
BstPI 510 GGTTACC G^GT[ACGT]ACC
Bsu36I 1725 CCTTAGG CC^T[ACGT]AGG
CfoI 1680 GCGC GCG^C
Eam1105I 1561 GACTATGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1725 CCTTAGG CC^T[ACGT]AGG
EcoO65I 510 GGTTACC G^GT[ACGT]ACC
EcoT22I 1767 ATGCAT ATGCA^T
FauI 1536 CCCGC CCCGC(4/6)
HaeII 1679 GGCGCC [GA]GCGC^[CT]
HhaI 1680 GCGC GCG^C
Hin1I 1679 GGCGCC G[GA]^CG[CT]C
HinP1I 1680 GCGC G^CGC
HincII 411 GTCAAC GT[CT]^[GA]AC
HindII 411 GTCAAC GT[CT]^[GA]AC
HindIII 713 AAGCTT A^AGCTT
KasI 1679 GGCGCC G^GCGCC
MaeII 1066 ACGT A^CGT
MroI 1465 TCCGGA T^CCGGA
MseI 872 TTAA T^TAA
MslI 1770 CATTGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 1734 GCCGGC GCC^GGC
NarI 1679 GGCGCC GG^CGCC
NciI 825 CCGGG CC^[GC]GG
NgoMIV 1734 GCCGGC G^CCGGC
NheI 729 GCTAGC G^CTAGC
NsiI 1767 ATGCAT ATGCA^T
PflMI 15 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SfoI 1679 GGCGCC GGC^GCC
SspI 878 AATATT AAT^ATT
Tru9I 872 TTAA T^TAA
Van91I 15 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1688 CCAAAGGGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccI 1175 GTCTAC GT^[AC][GT]AC
1214 GTCTAC
AlwI 180 GATCC GGATC(4/5)
1790 GGATC
AspHI 842 GTGCTC G[AT]GC[AT]^C
1285 GTGCAC
AvaII 1023 GGTCC G^G[AT]CC
1039 GGACC
BalI 255 TGGCCA TGG^CCA
1553 TGGCCA
BanI 1679 GGCGCC G^G[CT][GA]CC
1705 GGCACC
BanII 1372 GAGCCC G[GA]GC[CT]^C
1761 GAGCCC
BbsI 1195 GAAGAC GAAGAC(2/6)
1546 GAAGAC
BglII 38 AGATCT A^GATCT
1422 AGATCT
BlpI 524 GCTTAGC GC^T[ACGT]AGC
1150 GCTAAGC
Bpu1102I 524 GCTTAGC GC^T[ACGT]AGC
1150 GCTAAGC
BpuAI 1195 GAAGAC GAAGAC(2/6)
1546 GAAGAC
BsgI 144 CTGCAC GTGCAG(16/14)
1777 GTGCAG
BsiHKAI 842 GTGCTC G[AT]GC[AT]^C
1285 GTGCAC
BsrFI 1109 ACCGGC [GA]^CCGG[CT]
1734 GCCGGC
CelII 524 GCTTAGC GC^T[ACGT]AGC
1150 GCTAAGC
Cfr10I 1109 ACCGGC [GA]^CCGG[CT]
1734 GCCGGC
EarI 313 GAAGAG CTCTTC(1/4)
994 GAAGAG
HphI 439 TCACC GGTGA(8/7)
1598 TCACC
Ksp632I 313 GAAGAG CTCTTC(1/4)
994 GAAGAG
MluNI 255 TGGCCA TGG^CCA
1553 TGGCCA
MscI 255 TGGCCA TGG^CCA
1553 TGGCCA
PaeR7I 814 CTCGAG C^TCGAG
1337 CTCGAG
PleI 1718 GACTC GAGTC(4/5)
1567 GAGTC
PstI 34 CTGCAG CTGCA^G
1377 CTGCAG
PvuII 19 CAGCTG CAG^CTG
1588 CAGCTG
SfcI 34 CTGCAG C^T[GA][CT]AG
1377 CTGCAG
Tsp45I 751 GTCAC ^GT[GC]AC
1597 GTCAC
XhoI 814 CTCGAG C^TCGAG
1337 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AciI AfaI AluI AvaI BbvI
BfaI BmyI BsaJI BseRI BsiYI
BslI BsmAI BsoBI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII EaeI
EcoRII Fnu4HI FokI HaeIII HapII
HinfI HpaII ItaI MaeI MaeIII
MboI MboII MflI MnlI MspA1I
MspI MvaI MwoI NdeII NlaIII
NlaIV RsaI Sau3AI Sau96I ScrFI
SfaNI SmlI TaqI TfiI TseI
Tsp509I TspRI TthHB8I XhoII