Restriction map for
pF1KB6406
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# NO CUT ENZYMES ( 154 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AflIII AgeI AhdI
Alw44I AlwI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspHI AspI AviII AvrII
BalI BamHI BbrPI BbsI BbvCI
BcgI BciVI BclI BfrI BglI
BlnI BlpI Bpu1102I BpuAI BsaBI
BsaI BseAI BseRI BsiEI BsiHKAI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrFI BsrGI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO109I EcoO65I
EcoT22I FauI FbaI FseI FspI
HaeIII HgaI HincII HindII HindIII
HpaI KpnI KspI MamI MfeI
MluI MluNI MroI MscI MspA1I
MunI MvnI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfuI SgrAI SmaI SpeI SphI
Sse8387I SspBI StuI SwaI Tth111I
Van91I XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AciI 19 CCGC CCGC(-3/-1)
AcsI 446 GAATTC [GA]^AATT[CT]
AcyI 15 GGCGCC G[GA]^CG[CT]C
AlwNI 125 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 446 GAATTC [GA]^AATT[CT]
Asp700I 256 GAAAAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 302 CTCGAG C^[CT]CG[GA]G
BanI 15 GGCGCC G^G[CT][GA]CC
BanII 848 GGGCTC G[GA]GC[CT]^C
BbeI 15 GGCGCC GGCGC^C
BcnI 981 CCGGG CC^[GC]GG
BglII 369 AGATCT A^GATCT
BmyI 848 GGGCTC G[AGT]GC[ACT]^C
BsaAI 245 TACGTA [CT]AC^GT[GA]
BsaHI 15 GGCGCC G[GA]^CG[CT]C
BsaWI 995 ACCGGA [AT]^CCGG[AT]
BsgI 551 GTGCAG GTGCAG(16/14)
BsoBI 302 CTCGAG C^[CT]CG[GA]G
Bsp1286I 848 GGGCTC G[AGT]GC[ACT]^C
BsrDI 519 CATTGC GCAATG(2/0)
BssSI 452 CTCGTG CACGAG(-5/-1)
BstNI 187 CCTGG CC^[AT]GG
BstYI 369 AGATCT [GA]^GATC[CT]
Cac8I 716 GCCAGC GC[ACGT]^[ACGT]GC
CfoI 16 GCGC GCG^C
EcoRI 446 GAATTC G^AATTC
EcoRII 187 CCTGG ^CC[AT]GG
EcoRV 766 GATATC GAT^ATC
HaeII 15 GGCGCC [GA]GCGC^[CT]
HhaI 16 GCGC GCG^C
Hin1I 15 GGCGCC G[GA]^CG[CT]C
HinP1I 16 GCGC G^CGC
KasI 15 GGCGCC G^GCGCC
MflI 369 AGATCT [GA]^GATC[CT]
MslI 45 CACCGACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI 187 CCTGG CC^[AT]GG
MwoI 408 GCGAATTGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NarI 15 GGCGCC GG^CGCC
NciI 981 CCGGG CC^[GC]GG
NspI 50 ACATGC [GA]CATG^[CT]
PaeR7I 302 CTCGAG C^TCGAG
SfaNI 127 GCATC GCATC(5/9)
SfcI 524 CTACAG C^T[GA][CT]AG
SfoI 15 GGCGCC GGC^GCC
SmlI 302 CTCGAG C^T[CT][GA]AG
SnaBI 245 TACGTA TAC^GTA
SspI 422 AATATT AAT^ATT
TaqI 303 TCGA T^CGA
TthHB8I 303 TCGA T^CGA
XhoI 302 CTCGAG C^TCGAG
XhoII 369 AGATCT [GA]^GATC[CT]
XmnI 256 GAAAAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AvaII 319 GGTCC G^G[AT]CC
993 GGACC
BbvI 124 GCAGC GCAGC(8/12)
328 GCTGC
BfaI 364 CTAG C^TAG
859 CTAG
BpmI 188 CTGGAG CTGGAG(16/14)
911 CTGGAG
BsaJI 899 CCTTGG C^C[ACGT][ACGT]GG
989 CCAAGG
BsmAI 25 GAGAC GTCTC(1/5)
694 GAGAC
BsmFI 431 GGGAC GGGAC(10/14)
896 GTCCC
BssKI 187 CCTGG ^CC[ACGT]GG
981 CCGGG
Cfr13I 319 GGTCC G^G[ACGT]CC
993 GGACC
DdeI 176 CTAAG C^T[ACGT]AG
373 CTCAG
DpnII 370 GATC ^GATC
787 GATC
EarI 457 GAAGAG CTCTTC(1/4)
545 GAAGAG
EcoT14I 899 CCTTGG C^C[AT][AT]GG
989 CCAAGG
HapII 981 CCGG C^CGG
996 CCGG
HpaII 981 CCGG C^CGG
996 CCGG
Ksp632I 457 GAAGAG CTCTTC(1/4)
545 GAAGAG
MaeI 364 CTAG C^TAG
859 CTAG
MaeII 83 ACGT A^CGT
246 ACGT
MboI 370 GATC ^GATC
787 GATC
MseI 356 TTAA T^TAA
497 TTAA
MspI 981 CCGG C^CGG
996 CCGG
NdeII 370 GATC ^GATC
787 GATC
NlaIV 15 GGCGCC GG[ACGT]^[ACGT]CC
984 GGAACC
Sau3AI 370 GATC ^GATC
787 GATC
Sau96I 319 GGTCC G^G[ACGT]CC
993 GGACC
ScrFI 187 CCTGG CC^[ACGT]GG
981 CCGGG
StyI 899 CCTTGG C^C[AT][AT]GG
989 CCAAGG
Tru9I 356 TTAA T^TAA
497 TTAA
TseI 124 GCAGC G^C[AT]GC
328 GCTGC
Tsp45I 566 GTGAC ^GT[GC]AC
907 GTCAC
XcmI 560 CCAGTGGTGACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
890 CCATCCGTCCCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 21 enzymes) #
#########################################
AfaI AluI BsiYI BslI BsrI
BstF5I EcoNI Fnu4HI FokI HinfI
HphI ItaI MaeIII MboII MnlI
NlaIII PleI RsaI TfiI Tsp509I
TspRI