Restriction map for
pF1KB7519
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# NO CUT ENZYMES ( 176 enzymes) #
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AatII Acc65I AccI AccIII AclI
AfaI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AvaII AviII
AvrII BamHI BanI BbeI BbrPI
BcgI BciVI BclI BcnI BfaI
BfrI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaWI BseAI BsiEI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspLU11I BspMI BsrBI
BsrFI BsrGI BssHII BssKI BssSI
Bst1107I BstAPI BstBI BstEII BstF5I
BstNI BstPI BstXI BstYI BstZ17I
Bsu36I CelII Cfr10I Cfr13I ClaI
CpoI DraI DraII DraIII DrdI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRII EcoT22I FbaI FokI
FseI FspI HaeII HapII HincII
HindII HpaI HpaII HphI KasI
KpnI Ksp632I MaeI MaeIII MamI
MfeI MflI MluI MroI MslI
MspI MunI MvaI NaeI NarI
NciI NcoI NgoMIV NheI NlaIV
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RsaI RsrII
SalI SapI Sau96I SbfI ScaI
ScrFI SexAI SfaNI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tsp45I Tth111I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 62 enzymes) #
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AccII 23 CGCG CG^CG
AcsI 99 AAATTC [GA]^AATT[CT]
AcyI 46 GACGCC G[GA]^CG[CT]C
AlwI 19 GATCC GGATC(4/5)
AlwNI 314 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 99 AAATTC [GA]^AATT[CT]
AspHI 166 GAGCTC G[AT]GC[AT]^C
BalI 299 TGGCCA TGG^CCA
BanII 166 GAGCTC G[GA]GC[CT]^C
BbsI 178 GTCTTC GAAGAC(2/6)
BbvCI 322 GCTGAGG CCTCAGC(-5/-2)
BglI 292 GCCTGTTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 166 GAGCTC G[AGT]GC[ACT]^C
BpmI 262 CTCCAG CTGGAG(16/14)
BpuAI 178 GTCTTC GAAGAC(2/6)
BsaHI 46 GACGCC G[GA]^CG[CT]C
BsaI 232 GAGACC GGTCTC(1/5)
BsgI 149 GTGCAG GTGCAG(16/14)
BsiHKAI 166 GAGCTC G[AT]GC[AT]^C
BsmFI 357 GGGAC GGGAC(10/14)
Bsp1286I 166 GAGCTC G[AGT]GC[ACT]^C
BspHI 182 TCATGA T^CATGA
BsrDI 237 CATTGC GCAATG(2/0)
BsrI 242 CCAGT ACTGG(1/-1)
BstUI 23 CGCG CG^CG
CfoI 411 GCGC GCG^C
DpnII 19 GATC ^GATC
DsaI 22 CCGCGG C^C[GA][CT]GG
EaeI 299 TGGCCA [CT]^GGCC[GA]
EcoRV 397 GATATC GAT^ATC
EcoT14I 332 CCAAGG C^C[AT][AT]GG
HgaI 46 GACGC GACGC(5/10)
HhaI 411 GCGC GCG^C
Hin1I 46 GACGCC G[GA]^CG[CT]C
HinP1I 411 GCGC G^CGC
KspI 22 CCGCGG CCGC^GG
MaeII 35 ACGT A^CGT
MboI 19 GATC ^GATC
MluNI 299 TGGCCA TGG^CCA
MscI 299 TGGCCA TGG^CCA
MseI 193 TTAA T^TAA
MvnI 23 CGCG CG^CG
MwoI 292 GCCTGTTTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeI 112 CATATG CA^TATG
NdeII 19 GATC ^GATC
NlaIII 183 CATG CATG^
PleI 328 GAGTC GAGTC(4/5)
PstI 379 CTGCAG CTGCA^G
PvuII 83 CAGCTG CAG^CTG
RcaI 182 TCATGA T^CATGA
SacI 166 GAGCTC GAGCT^C
SacII 22 CCGCGG CCGC^GG
Sau3AI 19 GATC ^GATC
SfcI 379 CTGCAG C^T[GA][CT]AG
SfiI 291 GGCCTGTTTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
StyI 332 CCAAGG C^C[AT][AT]GG
TaqI 129 TCGA T^CGA
TfiI 387 GATTC G^A[AT]TC
Tru9I 193 TTAA T^TAA
Tsp509I 100 AATT ^AATT
TspRI 243 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 129 TCGA T^CGA
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# TWO CUT ENZYMES ( 9 enzymes) #
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BsaJI 22 CCGCGG C^C[ACGT][ACGT]GG
332 CCAAGG
BsiYI 22 CCGCGGGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
345 CCCAAGCTTGG
BslI 22 CCGCGGGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
345 CCCAAGCTTGG
FauI 7 GCGGG CCCGC(4/6)
24 GCGGG
HaeIII 291 GGCC GG^CC
300 GGCC
HindIII 271 AAGCTT A^AGCTT
348 AAGCTT
HinfI 328 GAGTC G^A[ACGT]TC
387 GATTC
MboII 367 GAAGA GAAGA(8/7)
179 TCTTC
MspA1I 22 CCGCGG C[AC]G^C[GT]G
83 CAGCTG
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# 3 OR MORE CUT ENZYMES ( 11 enzymes) #
#########################################
AciI AluI BbvI BseRI BsmAI
Cac8I DdeI Fnu4HI ItaI MnlI
TseI