Restriction map for
pF1KE0475
##################################
# NO CUT ENZYMES ( 137 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspI AviII
AvrII BalI BamHI BbrPI BcgI
BciVI BclI BfrI BglI BlnI
BlpI Bpu1102I BsaAI BseAI BsiEI
BsiWI BsmBI BsmI BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraII DraIII DrdI EaeI EagI
EclXI Eco47III Eco52I Eco81I EcoO109I
EcoO65I EcoRV EcoT22I FbaI FseI
FspI HgaI HindIII HpaI KpnI
KspI MaeII MaeIII MfeI MluI
MluNI MroI MscI MslI MspA1I
MunI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SapI SbfI
SexAI SfiI SfuI SgrAI SmlI
SnaBI SpeI Sse8387I StuI SwaI
Tsp45I Tth111I Van91I XbaI XcmI
XhoI XmnI
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# ONE CUT ENZYMES ( 60 enzymes) #
###################################
AccI 556 GTCGAC GT^[AC][GT]AC
AccII 32 CGCG CG^CG
AcyI 20 GGCGCC G[GA]^CG[CT]C
AhdI 548 GACAGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 393 GGATC GGATC(4/5)
AspEI 548 GACAGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 734 GTGCTC G[AT]GC[AT]^C
AvaI 92 CCCGGG C^[CT]CG[GA]G
AvaII 156 GGACC G^G[AT]CC
BanI 20 GGCGCC G^G[CT][GA]CC
BanII 49 GAGCCC G[GA]GC[CT]^C
BbeI 20 GGCGCC GGCGC^C
BbsI 371 GTCTTC GAAGAC(2/6)
BbvCI 60 GCTGAGG CCTCAGC(-5/-2)
BglII 678 AGATCT A^GATCT
BpmI 569 CTGGAG CTGGAG(16/14)
BpuAI 371 GTCTTC GAAGAC(2/6)
BsaBI 673 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 20 GGCGCC G[GA]^CG[CT]C
BsaWI 493 TCCGGT [AT]^CCGG[AT]
BsiHKAI 734 GTGCTC G[AT]GC[AT]^C
BsmFI 90 GTCCC GGGAC(10/14)
BsoBI 92 CCCGGG C^[CT]CG[GA]G
Bsp1407I 306 TGTACA T^GTACA
BspMI 552 GCAGGT ACCTGC(4/8)
BsrGI 306 TGTACA T^GTACA
BstUI 32 CGCG CG^CG
DdeI 61 CTGAG C^T[ACGT]AG
Eam1105I 548 GACAGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 594 GAAGAG CTCTTC(1/4)
EcoNI 185 CCTTGGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 276 GAATTC G^AATTC
EcoT14I 185 CCTTGG C^C[AT][AT]GG
FauI 30 CCCGC CCCGC(4/6)
HaeII 20 GGCGCC [GA]GCGC^[CT]
Hin1I 20 GGCGCC G[GA]^CG[CT]C
HincII 556 GTCGAC GT[CT]^[GA]AC
HindII 556 GTCGAC GT[CT]^[GA]AC
HphI 96 GGTGA GGTGA(8/7)
KasI 20 GGCGCC G^GCGCC
Ksp632I 594 GAAGAG CTCTTC(1/4)
MamI 673 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 32 CGCG CG^CG
NarI 20 GGCGCC GG^CGCC
NlaIV 20 GGCGCC GG[ACGT]^[ACGT]CC
PacI 202 TTAATTAA TTAAT^TAA
PleI 364 GAGTC GAGTC(4/5)
SalI 556 GTCGAC G^TCGAC
ScaI 77 AGTACT AGT^ACT
SfaNI 651 GATGC GCATC(5/9)
SfcI 746 CTACAG C^T[GA][CT]AG
SfoI 20 GGCGCC GGC^GCC
SmaI 92 CCCGGG CCC^GGG
SphI 442 GCATGC GCATG^C
SspBI 306 TGTACA T^GTACA
SspI 283 AATATT AAT^ATT
StyI 185 CCTTGG C^C[AT][AT]GG
TfiI 450 GATTC G^A[AT]TC
XmaCI 92 CCCGGG C^CCGGG
XmaI 92 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AciI 31 CCGC CCGC(-3/-1)
116 CCGC
AcsI 276 GAATTC [GA]^AATT[CT]
713 AAATTT
AfaI 78 GTAC GT^AC
307 GTAC
ApoI 276 GAATTC [GA]^AATT[CT]
713 AAATTT
BcnI 92 CCCGG CC^[GC]GG
93 CCGGG
BsaI 130 GAGACC GGTCTC(1/5)
296 GAGACC
BseRI 73 GAGGAG GAGGAG(10/8)
646 GAGGAG
BsgI 428 CTGCAC GTGCAG(16/14)
140 GTGCAG
BstF5I 491 CATCC GGATG(2/0)
173 GGATG
BstYI 393 GGATCT [GA]^GATC[CT]
678 AGATCT
Cac8I 118 GCACGC GC[ACGT]^[ACGT]GC
442 GCATGC
DsaI 125 CCACGG C^C[GA][CT]GG
589 CCACGG
FokI 491 CATCC GGATG(9/13)
173 GGATG
HapII 93 CCGG C^CGG
494 CCGG
HinfI 364 GAGTC G^A[ACGT]TC
450 GATTC
HpaII 93 CCGG C^CGG
494 CCGG
MflI 393 GGATCT [GA]^GATC[CT]
678 AGATCT
MspI 93 CCGG C^CGG
494 CCGG
NciI 92 CCCGG CC^[GC]GG
93 CCGGG
NspI 56 ACATGC [GA]CATG^[CT]
442 GCATGC
RsaI 78 GTAC GT^AC
307 GTAC
TaqI 519 TCGA T^CGA
557 TCGA
TthHB8I 519 TCGA T^CGA
557 TCGA
XhoII 393 GGATCT [GA]^GATC[CT]
678 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AluI BbvI BfaI BmyI BsaJI
BsiYI BslI BsmAI Bsp1286I BsrI
BssKI BstNI CfoI Cfr13I DpnII
EcoRII Fnu4HI HaeIII HhaI HinP1I
ItaI MaeI MboI MboII MnlI
MseI MvaI MwoI NdeII NlaIII
Sau3AI Sau96I ScrFI Tru9I TseI
Tsp509I TspRI