Restriction map for
pF1KE0047
##################################
# NO CUT ENZYMES ( 110 enzymes) #
##################################
AatII AccI AccIII AclI AcyI
AflII AflIII AgeI AhdI AocI
Aor51HI ApaI AscI AseI AsnI
AspEI AvaI AviII BamHI BbeI
BbrPI BbsI BcgI BciVI BfrI
BpuAI BsaBI BsaHI BseAI BsiEI
BsiWI BsmBI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrGI
BssHII BssSI Bst1107I BstBI BstXI
BstZ17I Bsu36I ClaI CpoI DraI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoT22I FseI
FspI HgaI Hin1I HincII HindII
HindIII HpaI KasI KspI MamI
MluI MroI NarI NheI NotI
NsiI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AccII 1915 CGCG CG^CG
Alw44I 2252 GTGCAC G^TGCAC
ApaLI 2252 GTGCAC G^TGCAC
Asp700I 2043 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 172 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 1806 CCTAGG C^CTAGG
BbvCI 1827 GCTGAGG CCTCAGC(-5/-2)
BclI 1998 TGATCA T^GATCA
BglII 1713 AGATCT A^GATCT
BlnI 1806 CCTAGG C^CTAGG
BsaAI 97 TACGTG [CT]AC^GT[GA]
BsaWI 1228 ACCGGA [AT]^CCGG[AT]
BstAPI 1121 GCATCCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 1915 CGCG CG^CG
CfoI 93 GCGC GCG^C
DraIII 522 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRI 1486 GAATTC G^AATTC
FbaI 1998 TGATCA T^GATCA
HaeII 92 GGCGCT [GA]GCGC^[CT]
HhaI 93 GCGC GCG^C
HinP1I 93 GCGC G^CGC
MfeI 547 CAATTG C^AATTG
MslI 1460 CACATGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 547 CAATTG C^AATTG
MvnI 1915 CGCG CG^CG
NaeI 1202 GCCGGC GCC^GGC
NgoMIV 1202 GCCGGC G^CCGGC
NruI 1914 TCGCGA TCG^CGA
PflFI 172 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1028 CCATCCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Tth111I 172 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1028 CCATCCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 458 CCAAGAAACATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 2043 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
Acc65I 86 GGTACC G^GTACC
327 GGTACC
AcsI 1486 GAATTC [GA]^AATT[CT]
1726 GAATTT
AlwNI 1268 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG
2232 CAGTGGCTG
ApoI 1486 GAATTC [GA]^AATT[CT]
1726 GAATTT
Asp718I 86 GGTACC G^GTACC
327 GGTACC
BcnI 492 CCGGG CC^[GC]GG
1160 CCCGG
BlpI 1253 GCTCAGC GC^T[ACGT]AGC
1850 GCTCAGC
Bpu1102I 1253 GCTCAGC GC^T[ACGT]AGC
1850 GCTCAGC
BsaI 1972 GAGACC GGTCTC(1/5)
495 GGTCTC
BsgI 1016 CTGCAC GTGCAG(16/14)
1600 GTGCAG
BsmFI 242 GGGAC GGGAC(10/14)
1023 GGGAC
BsrFI 89 ACCGGC [GA]^CCGG[CT]
1202 GCCGGC
BstEII 1224 GGTGACC G^GT[ACGT]ACC
1620 GGTGACC
BstPI 1224 GGTGACC G^GT[ACGT]ACC
1620 GGTGACC
BstYI 653 AGATCC [GA]^GATC[CT]
1713 AGATCT
CelII 1253 GCTCAGC GC^T[ACGT]AGC
1850 GCTCAGC
Cfr10I 89 ACCGGC [GA]^CCGG[CT]
1202 GCCGGC
EcoO65I 1224 GGTGACC G^GT[ACGT]ACC
1620 GGTGACC
EcoRV 1327 GATATC GAT^ATC
2137 GATATC
KpnI 86 GGTACC GGTAC^C
327 GGTACC
MaeII 98 ACGT A^CGT
434 ACGT
MflI 653 AGATCC [GA]^GATC[CT]
1713 AGATCT
NciI 492 CCGGG CC^[GC]GG
1160 CCCGG
NdeI 605 CATATG CA^TATG
730 CATATG
NspI 1439 ACATGC [GA]CATG^[CT]
1862 ACATGC
PpuMI 1023 GGGACCC [GA]G^G[AT]CC[CT]
1111 AGGTCCT
PstI 1128 CTGCAG CTGCA^G
2238 CTGCAG
PvuII 475 CAGCTG CAG^CTG
1837 CAGCTG
SapI 995 GAAGAGC GCTCTTC(1/4)
1008 GAAGAGC
SmlI 1352 CTCAAG C^T[CT][GA]AG
1767 CTTGAG
StuI 1215 AGGCCT AGG^CCT
2156 AGGCCT
XhoII 653 AGATCC [GA]^GATC[CT]
1713 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaII BalI BanI BanII BbvI
BfaI BglI BmyI BpmI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmI Bsp1286I BspMI BsrBI BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MluNI MnlI
MscI MseI MspA1I MspI MvaI
MwoI NcoI NdeII NlaIII NlaIV
PleI RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I