Restriction map for
pF1KB7064
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# NO CUT ENZYMES ( 109 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AflIII AgeI Alw44I Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspI AviII BbeI
BbrPI BciVI BclI BfrI BglII
BsaAI BsaBI BsaI BsgI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspLU11I BspMI BsrDI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstZ17I ClaI CpoI DraI
EagI EclXI Eco47III Eco52I EcoO65I
EcoRI EcoT22I FbaI FspI HindIII
KasI KpnI KspI MaeII MamI
MfeI MluI MunI NarI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tth111I XhoI XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AccII 91 CGCG CG^CG
AccIII 1632 TCCGGA T^CCGGA
AfaI 1537 GTAC GT^AC
AhdI 766 GACCCCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 766 GACCCCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 416 GTGCTC G[AT]GC[AT]^C
AvrII 1573 CCTAGG C^CTAGG
BamHI 640 GGATCC G^GATCC
BanI 908 GGTGCC G^G[CT][GA]CC
BglI 104 GCCTGAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 1573 CCTAGG C^CTAGG
BsaWI 1632 TCCGGA [AT]^CCGG[AT]
BseAI 1632 TCCGGA T^CCGGA
BsiHKAI 416 GTGCTC G[AT]GC[AT]^C
BspEI 1632 TCCGGA T^CCGGA
BspHI 1626 TCATGA T^CATGA
BsrBI 1971 CCGCTC CCGCTC(-3/-3)
BsrFI 1716 GCCGGC [GA]^CCGG[CT]
BstAPI 1987 GCAGCGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 91 CGCG CG^CG
BstYI 640 GGATCC [GA]^GATC[CT]
Cfr10I 1716 GCCGGC [GA]^CCGG[CT]
DraIII 1001 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 735 GACCTCCGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 766 GACCCCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1138 CCTGCTACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 1448 GATATC GAT^ATC
FseI 1715 GGCCGGCC GGCCGG^CC
HaeII 1607 AGCGCC [GA]GCGC^[CT]
HincII 520 GTTAAC GT[CT]^[GA]AC
HindII 520 GTTAAC GT[CT]^[GA]AC
HpaI 520 GTTAAC GTT^AAC
MflI 640 GGATCC [GA]^GATC[CT]
MroI 1632 TCCGGA T^CCGGA
MseI 521 TTAA T^TAA
MslI 654 CACTTGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 91 CGCG CG^CG
NaeI 1716 GCCGGC GCC^GGC
NgoMIV 1716 GCCGGC G^CCGGC
NspI 347 GCATGT [GA]CATG^[CT]
PstI 1985 CTGCAG CTGCA^G
PvuII 956 CAGCTG CAG^CTG
RcaI 1626 TCATGA T^CATGA
RsaI 1537 GTAC GT^AC
SmaI 1832 CCCGGG CCC^GGG
TaqI 1452 TCGA T^CGA
Tru9I 521 TTAA T^TAA
Tsp509I 398 AATT ^AATT
TthHB8I 1452 TCGA T^CGA
XbaI 123 TCTAGA T^CTAGA
XcmI 1552 CCACCGCCTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 640 GGATCC [GA]^GATC[CT]
XmaCI 1832 CCCGGG C^CCGGG
XmaI 1832 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcyI 230 GACGCC G[GA]^CG[CT]C
798 GGCGTC
AocI 105 CCTGAGG CC^T[ACGT]AGG
1854 CCTCAGG
ApaI 1427 GGGCCC GGGCC^C
1737 GGGCCC
AvaI 1757 CTCGGG C^[CT]CG[GA]G
1832 CCCGGG
BcgI 1749 GCAGCTTGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1766 GCAGCCGCTTCG
BlpI 1504 GCTGAGC GC^T[ACGT]AGC
1806 GCTGAGC
Bpu1102I 1504 GCTGAGC GC^T[ACGT]AGC
1806 GCTGAGC
BsaHI 230 GACGCC G[GA]^CG[CT]C
798 GGCGTC
BsoBI 1757 CTCGGG C^[CT]CG[GA]G
1832 CCCGGG
Bsu36I 105 CCTGAGG CC^T[ACGT]AGG
1854 CCTCAGG
CelII 1504 GCTGAGC GC^T[ACGT]AGC
1806 GCTGAGC
Eco81I 105 CCTGAGG CC^T[ACGT]AGG
1854 CCTCAGG
FauI 1730 CCCGC CCCGC(4/6)
1970 CCCGC
HgaI 230 GACGC GACGC(5/10)
799 GCGTC
Hin1I 230 GACGCC G[GA]^CG[CT]C
798 GGCGTC
MaeIII 1085 GTGAC ^GT[ACGT]AC
1684 GTCAC
MspA1I 956 CAGCTG C[AC]G^C[GT]G
1988 CAGCGG
NlaIII 348 CATG CATG^
1627 CATG
PpuMI 117 GGGACCT [GA]G^G[AT]CC[CT]
772 AGGTCCT
SfcI 1142 CTACAG C^T[GA][CT]AG
1985 CTGCAG
StuI 1019 AGGCCT AGG^CCT
1289 AGGCCT
TfiI 684 GAATC G^A[AT]TC
1366 GATTC
Tsp45I 1085 GTGAC ^GT[GC]AC
1684 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AciI AluI AlwI AlwNI AvaII
BalI BanII BbsI BbvCI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaJI BseRI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MboI MboII MluNI MnlI MscI
MspI MvaI MwoI NciI NdeII
NlaIV PflMI PleI Sau3AI Sau96I
ScrFI SfaNI SmlI StyI TseI
TspRI Van91I