Restriction map for
pF1KB6497
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# NO CUT ENZYMES ( 128 enzymes) #
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AatII Acc65I AccI AclI AcsI
AcyI AflII AflIII AgeI AhdI
Alw44I ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AspI AviII
BalI BamHI BbeI BbrPI BbsI
BbvCI BciVI BclI BfrI BglI
BlpI Bpu1102I BpuAI BsaHI BsgI
BsiWI BsmI Bsp1407I BspDI BspHI
BspLU11I BsrDI BsrGI BssHII BssSI
Bst1107I BstEII BstPI BstXI BstZ17I
CelII ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco52I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI Hin1I HincII HindII
HindIII HpaI KasI KpnI KspI
MfeI MluI MluNI MscI MslI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PshAI PshBI Psp1406I RcaI RsrII
SacII SalI ScaI SfiI SfoI
SgrAI SmaI SnaBI SpeI SphI
SspBI SspI StuI SwaI Tsp509I
TspRI Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccIII 1007 TCCGGA T^CCGGA
AlwNI 959 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 852 CCTCAGG CC^T[ACGT]AGG
Aor51HI 839 AGCGCT AGC^GCT
ApaI 668 GGGCCC GGGCC^C
Asp700I 48 GAACGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 672 CCTAGG C^CTAGG
BanI 185 GGCACC G^G[CT][GA]CC
BcgI 420 CGAGCCCCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 373 CCGGG CC^[GC]GG
BglII 737 AGATCT A^GATCT
BlnI 672 CCTAGG C^CTAGG
BpmI 319 CTCCAG CTGGAG(16/14)
BsaAI 367 TACGTG [CT]AC^GT[GA]
BsaI 193 GAGACC GGTCTC(1/5)
BsaWI 1007 TCCGGA [AT]^CCGG[AT]
BseAI 1007 TCCGGA T^CCGGA
BsiEI 293 CGATCG CG[GA][CT]^CG
BsmBI 753 CGTCTC CGTCTC(1/5)
BsmFI 601 GGGAC GGGAC(10/14)
BspEI 1007 TCCGGA T^CCGGA
BsrBI 101 CCGCTC CCGCTC(-3/-3)
BsrFI 196 ACCGGC [GA]^CCGG[CT]
BstAPI 801 GCATCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 130 TTCGAA TT^CGAA
Bsu36I 852 CCTCAGG CC^T[ACGT]AGG
Cfr10I 196 ACCGGC [GA]^CCGG[CT]
EarI 1000 GAAGAG CTCTTC(1/4)
Eco47III 839 AGCGCT AGC^GCT
Eco81I 852 CCTCAGG CC^T[ACGT]AGG
EcoNI 816 CCTGCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 839 AGCGCT [GA]GCGC^[CT]
HinfI 513 GAATC G^A[ACGT]TC
Ksp632I 1000 GAAGAG CTCTTC(1/4)
MaeII 368 ACGT A^CGT
MroI 1007 TCCGGA T^CCGGA
MseI 332 TTAA T^TAA
MspA1I 903 CAGCTG C[AC]G^C[GT]G
NciI 373 CCGGG CC^[GC]GG
NspV 130 TTCGAA TT^CGAA
PpuMI 601 GGGACCC [GA]G^G[AT]CC[CT]
PvuI 293 CGATCG CGAT^CG
PvuII 903 CAGCTG CAG^CTG
SacI 1003 GAGCTC GAGCT^C
SapI 1000 GAAGAGC GCTCTTC(1/4)
SexAI 353 ACCTGGT A^CC[AT]GGT
SfuI 130 TTCGAA TT^CGAA
SmlI 796 CTCAAG C^T[CT][GA]AG
TfiI 513 GAATC G^A[AT]TC
Tru9I 332 TTAA T^TAA
Tsp45I 547 GTGAC ^GT[GC]AC
XmnI 48 GAACGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 16 enzymes) #
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AvaI 508 CCCGAG C^[CT]CG[GA]G
622 CCCGAG
BbvI 807 GCTGC GCAGC(8/12)
819 GCTGC
BfaI 58 CTAG C^TAG
673 CTAG
BsaBI 909 GATCGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1011 GATCTCCATC
BsmAI 193 GAGAC GTCTC(1/5)
754 GTCTC
BsoBI 508 CCCGAG C^[CT]CG[GA]G
622 CCCGAG
DsaI 656 CCGTGG C^C[GA][CT]GG
698 CCGTGG
EcoT14I 672 CCTAGG C^C[AT][AT]GG
1034 CCAAGG
HgaI 144 GACGC GACGC(5/10)
404 GCGTC
MaeI 58 CTAG C^TAG
673 CTAG
MaeIII 547 GTGAC ^GT[ACGT]AC
977 GTAAC
MamI 909 GATCGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1011 GATCTCCATC
SbfI 285 CCTGCAGG CCTGCA^GG
540 CCTGCAGG
Sse8387I 285 CCTGCAGG CCTGCA^GG
540 CCTGCAGG
StyI 672 CCTAGG C^C[AT][AT]GG
1034 CCAAGG
TseI 807 GCTGC G^C[AT]GC
819 GCTGC
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# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AspHI AvaII BanII BmyI BsaJI
BseRI BsiHKAI BsiYI BslI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII EaeI EcoO109I
EcoRII FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HpaII HphI
ItaI MboI MboII MflI MnlI
MspI MvaI MvnI MwoI NdeII
NlaIII NlaIV PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI TaqI
TthHB8I XhoII