Restriction map for
pF1KB6455
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# NO CUT ENZYMES ( 146 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AlwNI Aor51HI ApaI ApaLI AscI
Asp700I Asp718I AspEI AspHI AspI
AvaI AviII AvrII BamHI BanII
BbrPI BbsI BbvCI BcgI BciVI
BfrI BglI BglII BlnI BlpI
BmyI Bpu1102I BpuAI BseAI BseRI
BsiEI BsiHKAI BsiWI BsmBI BsmI
BsoBI Bsp1286I Bsp1407I BspDI BspEI
BspLU11I BsrBI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstYI BstZ17I CelII ClaI CpoI
DraI DraII DraIII DrdI EagI
Eam1105I EarI EclXI Eco47III Eco52I
EcoNI EcoO109I EcoO65I EcoRI EcoT22I
FauI FseI FspI HgaI HincII
HindII HpaI KpnI Ksp632I KspI
MfeI MflI MluI MroI MunI
MvnI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI Psp1406I PvuI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfuI SgrAI
SmaI SmlI SnaBI SpeI Sse8387I
SspBI SspI StuI SwaI TaqI
TspRI Tth111I TthHB8I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AfaI 142 GTAC GT^AC
AocI 826 CCTCAGG CC^T[ACGT]AGG
AseI 220 ATTAAT AT^TAAT
AsnI 220 ATTAAT AT^TAAT
BalI 644 TGGCCA TGG^CCA
BclI 212 TGATCA T^GATCA
BfaI 79 CTAG C^TAG
BpmI 73 CTGGAG CTGGAG(16/14)
BsaAI 143 TACGTG [CT]AC^GT[GA]
BsaBI 867 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 115 GAGACC GGTCTC(1/5)
BsaWI 760 TCCGGT [AT]^CCGG[AT]
BsmFI 946 GTCCC GGGAC(10/14)
BspHI 215 TCATGA T^CATGA
BspMI 355 GCAGGT ACCTGC(4/8)
BsrFI 444 GCCGGC [GA]^CCGG[CT]
BsrI 421 CCAGT ACTGG(1/-1)
BstAPI 680 GCACAATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 826 CCTCAGG CC^T[ACGT]AGG
Cfr10I 444 GCCGGC [GA]^CCGG[CT]
DsaI 377 CCATGG C^C[GA][CT]GG
EaeI 644 TGGCCA [CT]^GGCC[GA]
Eco81I 826 CCTCAGG CC^T[ACGT]AGG
EcoRV 415 GATATC GAT^ATC
EcoT14I 377 CCATGG C^C[AT][AT]GG
FbaI 212 TGATCA T^GATCA
HindIII 328 AAGCTT A^AGCTT
MaeI 79 CTAG C^TAG
MaeII 144 ACGT A^CGT
MamI 867 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 644 TGGCCA TGG^CCA
MscI 644 TGGCCA TGG^CCA
NaeI 444 GCCGGC GCC^GGC
NcoI 377 CCATGG C^CATGG
NgoMIV 444 GCCGGC G^CCGGC
PleI 744 GACTC GAGTC(4/5)
PshBI 220 ATTAAT AT^TAAT
PstI 257 CTGCAG CTGCA^G
RcaI 215 TCATGA T^CATGA
RsaI 142 GTAC GT^AC
SphI 790 GCATGC GCATG^C
StyI 377 CCATGG C^C[AT][AT]GG
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# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccI 157 GTCTAC GT^[AC][GT]AC
424 GTAGAC
AcsI 100 AAATTC [GA]^AATT[CT]
398 AAATTC
AcyI 312 GGCGCC G[GA]^CG[CT]C
718 GGCGCC
AlwI 882 GATCC GGATC(4/5)
636 GGATC
ApoI 100 AAATTC [GA]^AATT[CT]
398 AAATTC
BanI 312 GGCGCC G^G[CT][GA]CC
718 GGCGCC
BbeI 312 GGCGCC GGCGC^C
718 GGCGCC
BbvI 26 GCAGC GCAGC(8/12)
694 GCAGC
BsaHI 312 GGCGCC G[GA]^CG[CT]C
718 GGCGCC
BsaJI 66 CCTGGG C^C[ACGT][ACGT]GG
377 CCATGG
BsgI 492 CTGCAC GTGCAG(16/14)
359 GTGCAG
BsiYI 66 CCTGGGCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
508 CCTGATGCTGG
BslI 66 CCTGGGCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
508 CCTGATGCTGG
BsmAI 115 GAGAC GTCTC(1/5)
502 GTCTC
BsrDI 540 CATTGC GCAATG(2/0)
847 CATTGC
BstXI 596 CCAATGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
647 CCATCCTTGTGG
CfoI 313 GCGC GCG^C
719 GCGC
HaeII 312 GGCGCC [GA]GCGC^[CT]
718 GGCGCC
HhaI 313 GCGC GCG^C
719 GCGC
Hin1I 312 GGCGCC G[GA]^CG[CT]C
718 GGCGCC
HinP1I 313 GCGC G^CGC
719 GCGC
KasI 312 GGCGCC G^GCGCC
718 GGCGCC
MseI 199 TTAA T^TAA
221 TTAA
MslI 648 CATCCTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
873 CATCTCTATG
NarI 312 GGCGCC GG^CGCC
718 GGCGCC
NlaIV 312 GGCGCC GG[ACGT]^[ACGT]CC
718 GGCGCC
NspI 387 ACATGC [GA]CATG^[CT]
790 GCATGC
PvuII 695 CAGCTG CAG^CTG
713 CAGCTG
SfaNI 511 GATGC GCATC(5/9)
779 GCATC
SfcI 257 CTGCAG C^T[GA][CT]AG
350 CTGTAG
SfoI 312 GGCGCC GGC^GCC
718 GGCGCC
Tru9I 199 TTAA T^TAA
221 TTAA
TseI 26 GCAGC G^C[AT]GC
694 GCAGC
#########################################
# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AciI AluI AvaII BcnI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII EcoRII Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
MaeIII MboI MboII MnlI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII Sau3AI Sau96I ScrFI TfiI
Tsp45I Tsp509I