Restriction map for
pF1KB6371
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# NO CUT ENZYMES ( 170 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflIII AgeI AhdI
Alw44I AlwI AocI Aor51HI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BalI BamHI BanI BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BcnI BglII BlnI BpmI
BpuAI BsaAI BsaBI BsaHI BsaWI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiWI BsmFI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrFI BsrGI BsrI BssHII BssSI
Bst1107I BstEII BstF5I BstPI BstUI
BstXI BstYI BstZ17I Bsu36I Cfr10I
ClaI CpoI DpnII DraIII DsaI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FbaI FokI
FseI FspI HaeII Hin1I HindIII
HpaI KasI KpnI Ksp632I KspI
MaeII MamI MboI MflI MluI
MluNI MroI MscI MslI MspA1I
MvnI NaeI NarI NciI NcoI
NdeII NgoMIV NheI NotI NruI
NsiI NspI PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI Sau3AI SbfI ScaI SexAI
SfiI SfoI SgrAI SmaI SnaBI
SphI Sse8387I SspBI SspI StuI
StyI Tth111I Van91I XbaI XcmI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AciI 241 GCGG CCGC(-3/-1)
AfaI 524 GTAC GT^AC
AflII 600 CTTAAG C^TTAAG
AlwNI 625 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 438 GGGCCC GGGCC^C
AvaII 627 GGACC G^G[AT]CC
BfrI 600 CTTAAG C^TTAAG
BglI 59 GCCCCCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 6 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 6 GCTCAGC GC^T[ACGT]AGC
BsiYI 62 CCCAGGGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 62 CCCAGGGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmBI 607 GAGACG CGTCTC(1/5)
BsrDI 91 GCAATG GCAATG(2/0)
BstAPI 748 GCACAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 23 TTCGAA TT^CGAA
CelII 6 GCTCAGC GC^T[ACGT]AGC
CfoI 172 GCGC GCG^C
DdeI 7 CTCAG C^T[ACGT]AG
DraI 567 TTTAAA TTT^AAA
DrdI 37 GACAATGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 387 TGGCCG [CT]^GGCC[GA]
FauI 241 GCGGG CCCGC(4/6)
HapII 390 CCGG C^CGG
HhaI 172 GCGC GCG^C
HinP1I 172 GCGC G^CGC
HincII 214 GTTGAC GT[CT]^[GA]AC
HindII 214 GTTGAC GT[CT]^[GA]AC
HpaII 390 CCGG C^CGG
HphI 15 GGTGA GGTGA(8/7)
MaeIII 46 GTCAC ^GT[ACGT]AC
MboII 727 GAAGA GAAGA(8/7)
MfeI 236 CAATTG C^AATTG
MnlI 442 CCTC CCTC(7/6)
MspI 390 CCGG C^CGG
MunI 236 CAATTG C^AATTG
NspV 23 TTCGAA TT^CGAA
PpuMI 626 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 539 GACATATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsaI 524 GTAC GT^AC
SfaNI 256 GATGC GCATC(5/9)
SfuI 23 TTCGAA TT^CGAA
SmlI 600 CTTAAG C^T[CT][GA]AG
SpeI 576 ACTAGT A^CTAGT
SwaI 566 ATTTAAAT ATTT^AAAT
Tsp45I 46 GTCAC ^GT[GC]AC
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# TWO CUT ENZYMES ( 26 enzymes) #
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AcsI 203 AAATTC [GA]^AATT[CT]
550 GAATTT
ApoI 203 AAATTC [GA]^AATT[CT]
550 GAATTT
BbvI 3 GCAGC GCAGC(8/12)
187 GCAGC
BfaI 260 CTAG C^TAG
577 CTAG
BsaI 393 GAGACC GGTCTC(1/5)
409 GGTCTC
BsaJI 62 CCCAGG C^C[ACGT][ACGT]GG
63 CCAGGG
BspMI 762 ACCTGC ACCTGC(4/8)
774 ACCTGC
BssKI 63 CCAGG ^CC[ACGT]GG
714 CCTGG
BstNI 63 CCAGG CC^[AT]GG
714 CCTGG
DraII 438 GGGCCCT [GA]G^G[ACGT]CC[CT]
626 AGGACCT
EcoO109I 438 GGGCCCT [GA]G^G[ACGT]CC[CT]
626 AGGACCT
EcoRII 63 CCAGG ^CC[AT]GG
714 CCTGG
Fnu4HI 3 GCAGC GC^[ACGT]GC
187 GCAGC
HaeIII 388 GGCC GG^CC
439 GGCC
HgaI 609 GACGC GACGC(5/10)
282 GCGTC
HinfI 72 GATTC G^A[ACGT]TC
298 GATTC
ItaI 3 GCAGC GC^[ACGT]GC
187 GCAGC
MaeI 260 CTAG C^TAG
577 CTAG
MvaI 63 CCAGG CC^[AT]GG
714 CCTGG
NdeI 398 CATATG CA^TATG
541 CATATG
ScrFI 63 CCAGG CC^[ACGT]GG
714 CCTGG
TaqI 24 TCGA T^CGA
314 TCGA
TfiI 72 GATTC G^A[AT]TC
298 GATTC
TseI 3 GCAGC G^C[AT]GC
187 GCAGC
TspRI 48 CACTG CA[GC]TG[ACGT][ACGT]^
323 CACTG
TthHB8I 24 TCGA T^CGA
314 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 16 enzymes) #
#########################################
AluI BanII BmyI BsmAI Bsp1286I
Cac8I Cfr13I MseI MwoI NlaIII
NlaIV PstI Sau96I SfcI Tru9I
Tsp509I