Restriction map for pF1KB6813
##################################
# NO CUT ENZYMES (  192 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AciI        
AclI        AcyI        AflII       AgeI        AhdI        
Alw44I      AlwNI       AocI        Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspHI       AvaI        AvaII       
AviII       AvrII       BamHI       BanI        BanII       
BbeI        BbrPI       BbvCI       BcgI        BciVI       
BclI        BcnI        BfaI        BfrI        BglI        
BlnI        BlpI        BmyI        Bpu1102I    BsaAI       
BsaBI       BsaHI       BsaI        BsaWI       BseAI       
BseRI       BsgI        BsiHKAI     BsiWI       BsiYI       
BslI        BsmBI       BsmI        BsoBI       Bsp1286I    
Bsp1407I    BspDI       BspEI       BspHI       BspMI       
BsrBI       BsrDI       BsrFI       BsrGI       BssHII      
BssKI       BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstNI       BstPI       BstUI       BstXI       
BstZ17I     Bsu36I      CelII       CfoI        Cfr10I      
Cfr13I      ClaI        CpoI        DraII       DraIII      
DrdI        DsaI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoRII      EcoRV       
EcoT22I     FauI        FbaI        FseI        FspI        
HaeII       HapII       HgaI        HhaI        Hin1I       
HinP1I      HpaII       KasI        KpnI        Ksp632I     
KspI        MaeI        MaeII       MaeIII      MamI        
MfeI        MluI        MroI        MspA1I      MspI        
MunI        MvaI        MvnI        NaeI        NarI        
NciI        NcoI        NdeI        NgoMIV      NheI        
NlaIV       NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflMI       PinAI       PleI        
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuII       RcaI        
RsrII       SacI        SacII       SapI        Sau96I      
SbfI        ScaI        ScrFI       SexAI       SfaNI       
SfcI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        Tsp45I      
Van91I      XbaI        XcmI        XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   36 enzymes) #
###################################

AccI          301  GTCGAC               GT^[AC][GT]AC
AfaI           24  GTAC                 GT^AC
AflIII        347  ACATGT               A^C[GA][CT]GT
AluI          284  AGCT                 AG^CT
AlwI          178  GATCC                GGATC(4/5)
AspI           37  GACTATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbvI            3  GCAGC                GCAGC(8/12)
BglII         471  AGATCT               A^GATCT
BpmI          475  CTGGAG               CTGGAG(16/14)
BsaJI         259  CCAAGG               C^C[ACGT][ACGT]GG
BsiEI          27  CGATCG               CG[GA][CT]^CG
BsmAI         119  GTCTC                GTCTC(1/5)
BsmFI          35  GGGAC                GGGAC(10/14)
BspLU11I      347  ACATGT               A^CATGT
BstYI         471  AGATCT               [GA]^GATC[CT]
Cac8I           6  GCCTGC               GC[ACGT]^[ACGT]GC
DraI          147  TTTAAA               TTT^AAA
EcoT14I       259  CCAAGG               C^C[AT][AT]GG
Fnu4HI          3  GCAGC                GC^[ACGT]GC
HindIII       283  AAGCTT               A^AGCTT
HpaI          264  GTTAAC               GTT^AAC
HphI          394  GGTGA                GGTGA(8/7)
ItaI            3  GCAGC                GC^[ACGT]GC
MflI          471  AGATCT               [GA]^GATC[CT]
MslI          145  CATTTAAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MwoI          285  GCTTAATTGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NspI          347  ACATGT               [GA]CATG^[CT]
PflFI          37  GACTATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PvuI           27  CGATCG               CGAT^CG
RsaI           24  GTAC                 GT^AC
SalI          301  GTCGAC               G^TCGAC
StyI          259  CCAAGG               C^C[AT][AT]GG
SwaI          146  ATTTAAAT             ATTT^AAAT
TseI            3  GCAGC                G^C[AT]GC
Tth111I        37  GACTATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoII         471  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AcsI           52  GAATTT               [GA]^AATT[CT]
               87  AAATTT              
ApoI           52  GAATTT               [GA]^AATT[CT]
               87  AAATTT              
BalI          246  TGGCCA               TGG^CCA
              256  TGGCCA              
BsrI          323  ACTGG                ACTGG(1/-1)
              249  CCAGT               
BstF5I         75  GGATG                GGATG(2/0)
              402  GGATG               
DdeI          295  CTTAG                C^T[ACGT]AG
              371  CTGAG               
EaeI          246  TGGCCA               [CT]^GGCC[GA]
              256  TGGCCA              
FokI           75  GGATG                GGATG(9/13)
              402  GGATG               
HaeIII        247  GGCC                 GG^CC
              257  GGCC                
HincII        264  GTTAAC               GT[CT]^[GA]AC
              301  GTCGAC              
HindII        264  GTTAAC               GT[CT]^[GA]AC
              301  GTCGAC              
MluNI         246  TGGCCA               TGG^CCA
              256  TGGCCA              
MnlI          125  GAGG                 CCTC(7/6)
              400  GAGG                
MscI          246  TGGCCA               TGG^CCA
              256  TGGCCA              
TaqI           30  TCGA                 T^CGA
              302  TCGA                
TspRI         169  CACTG                CA[GC]TG[ACGT][ACGT]^
              450  CAGTG               
TthHB8I        30  TCGA                 T^CGA
              302  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   13 enzymes) #
#########################################

BbsI        BpuAI       DpnII       HinfI       MboI        
MboII       MseI        NdeII       NlaIII      Sau3AI      
TfiI        Tru9I       Tsp509I     
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