################################## # NO CUT ENZYMES ( 192 enzymes) # ################################## AatII Acc65I AccII AccIII AciI AclI AcyI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AvaI AvaII AviII AvrII BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglI BlnI BlpI BmyI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiHKAI BsiWI BsiYI BslI BsmBI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssKI BssSI Bst1107I BstAPI BstBI BstEII BstNI BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I Cfr13I ClaI CpoI DraII DraIII DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRII EcoRV EcoT22I FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HpaII KasI KpnI Ksp632I KspI MaeI MaeII MaeIII MamI MfeI MluI MroI MspA1I MspI MunI MvaI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuII RcaI RsrII SacI SacII SapI Sau96I SbfI ScaI ScrFI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI Tsp45I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AccI 301 GTCGAC GT^[AC][GT]AC AfaI 24 GTAC GT^AC AflIII 347 ACATGT A^C[GA][CT]GT AluI 284 AGCT AG^CT AlwI 178 GATCC GGATC(4/5) AspI 37 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC BbvI 3 GCAGC GCAGC(8/12) BglII 471 AGATCT A^GATCT BpmI 475 CTGGAG CTGGAG(16/14) BsaJI 259 CCAAGG C^C[ACGT][ACGT]GG BsiEI 27 CGATCG CG[GA][CT]^CG BsmAI 119 GTCTC GTCTC(1/5) BsmFI 35 GGGAC GGGAC(10/14) BspLU11I 347 ACATGT A^CATGT BstYI 471 AGATCT [GA]^GATC[CT] Cac8I 6 GCCTGC GC[ACGT]^[ACGT]GC DraI 147 TTTAAA TTT^AAA EcoT14I 259 CCAAGG C^C[AT][AT]GG Fnu4HI 3 GCAGC GC^[ACGT]GC HindIII 283 AAGCTT A^AGCTT HpaI 264 GTTAAC GTT^AAC HphI 394 GGTGA GGTGA(8/7) ItaI 3 GCAGC GC^[ACGT]GC MflI 471 AGATCT [GA]^GATC[CT] MslI 145 CATTTAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MwoI 285 GCTTAATTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NspI 347 ACATGT [GA]CATG^[CT] PflFI 37 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC PvuI 27 CGATCG CGAT^CG RsaI 24 GTAC GT^AC SalI 301 GTCGAC G^TCGAC StyI 259 CCAAGG C^C[AT][AT]GG SwaI 146 ATTTAAAT ATTT^AAAT TseI 3 GCAGC G^C[AT]GC Tth111I 37 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoII 471 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AcsI 52 GAATTT [GA]^AATT[CT] 87 AAATTT ApoI 52 GAATTT [GA]^AATT[CT] 87 AAATTT BalI 246 TGGCCA TGG^CCA 256 TGGCCA BsrI 323 ACTGG ACTGG(1/-1) 249 CCAGT BstF5I 75 GGATG GGATG(2/0) 402 GGATG DdeI 295 CTTAG C^T[ACGT]AG 371 CTGAG EaeI 246 TGGCCA [CT]^GGCC[GA] 256 TGGCCA FokI 75 GGATG GGATG(9/13) 402 GGATG HaeIII 247 GGCC GG^CC 257 GGCC HincII 264 GTTAAC GT[CT]^[GA]AC 301 GTCGAC HindII 264 GTTAAC GT[CT]^[GA]AC 301 GTCGAC MluNI 246 TGGCCA TGG^CCA 256 TGGCCA MnlI 125 GAGG CCTC(7/6) 400 GAGG MscI 246 TGGCCA TGG^CCA 256 TGGCCA TaqI 30 TCGA T^CGA 302 TCGA TspRI 169 CACTG CA[GC]TG[ACGT][ACGT]^ 450 CAGTG TthHB8I 30 TCGA T^CGA 302 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 13 enzymes) # ######################################### BbsI BpuAI DpnII HinfI MboI MboII MseI NdeII NlaIII Sau3AI TfiI Tru9I Tsp509I