Restriction map for
pF1KE0159
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# NO CUT ENZYMES ( 137 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I ApaLI AseI AsnI Asp700I
Asp718I AspEI AspI AvaII AviII
AvrII BamHI BanI BbeI BbrPI
BbsI BclI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BseRI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrFI BsrGI BssSI Bst1107I
BstBI BstYI BstZ17I CelII Cfr10I
ClaI CpoI DraI DraII DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco52I EcoO109I EcoRV EcoT22I FbaI
FseI FspI HgaI Hin1I HincII
HindII HindIII HpaI KasI KpnI
KspI MaeII MamI MfeI MflI
MluI MroI MunI NaeI NarI
NcoI NgoMIV NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacII SalI SapI SbfI
ScaI SfiI SfoI SfuI SgrAI
SnaBI SpeI Sse8387I SspBI SspI
SwaI TfiI Tth111I XbaI XhoI
XhoII XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccII 29 CGCG CG^CG
AcsI 616 GAATTC [GA]^AATT[CT]
AlwI 644 GGATC GGATC(4/5)
AocI 583 CCTGAGG CC^T[ACGT]AGG
Aor51HI 145 AGCGCT AGC^GCT
ApaI 92 GGGCCC GGGCC^C
ApoI 616 GAATTC [GA]^AATT[CT]
AscI 27 GGCGCGCC GG^CGCGCC
AvaI 517 CCCGGG C^[CT]CG[GA]G
BalI 455 TGGCCA TGG^CCA
BbvCI 205 GCTGAGG CCTCAGC(-5/-2)
BcgI 81 GCAGGCTTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 555 GGATAC GTATCC(6/5)
BsgI 79 GTGCAG GTGCAG(16/14)
BsiEI 661 CGACCG CG[GA][CT]^CG
BsmAI 294 GTCTC GTCTC(1/5)
BsmFI 427 GGGAC GGGAC(10/14)
BsoBI 517 CCCGGG C^[CT]CG[GA]G
BsrDI 278 GCAATG GCAATG(2/0)
BssHII 28 GCGCGC G^CGCGC
BstAPI 409 GCAAAGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 29 CGCG CG^CG
BstXI 353 CCAGCCATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 583 CCTGAGG CC^T[ACGT]AGG
EaeI 455 TGGCCA [CT]^GGCC[GA]
Eco47III 145 AGCGCT AGC^GCT
Eco81I 583 CCTGAGG CC^T[ACGT]AGG
EcoNI 678 CCTAATCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 616 GAATTC G^AATTC
FauI 95 CCCGC CCCGC(4/6)
MluNI 455 TGGCCA TGG^CCA
MscI 455 TGGCCA TGG^CCA
MslI 531 CATCGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 504 CAGCGG C[AC]G^C[GT]G
MvnI 29 CGCG CG^CG
NdeI 333 CATATG CA^TATG
NheI 148 GCTAGC G^CTAGC
NlaIV 92 GGGCCC GG[ACGT]^[ACGT]CC
NspI 509 GCATGC [GA]CATG^[CT]
PflMI 483 CCAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 17 CTGCAG CTGCA^G
SacI 666 GAGCTC GAGCT^C
SexAI 194 ACCAGGT A^CC[AT]GGT
SfcI 17 CTGCAG C^T[GA][CT]AG
SmaI 517 CCCGGG CCC^GGG
SmlI 376 CTCAAG C^T[CT][GA]AG
SphI 509 GCATGC GCATG^C
StuI 387 AGGCCT AGG^CCT
TspRI 180 CACTG CA[GC]TG[ACGT][ACGT]^
Van91I 483 CCAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 517 CCCGGG C^CCGGG
XmaI 517 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AfaI 45 GTAC GT^AC
447 GTAC
AlwNI 484 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
597 CAGCTCCTG
AspHI 538 GTGCTC G[AT]GC[AT]^C
666 GAGCTC
BanII 92 GGGCCC G[GA]GC[CT]^C
666 GAGCTC
BfaI 149 CTAG C^TAG
622 CTAG
BpmI 351 CTCCAG CTGGAG(16/14)
260 CTGGAG
BsiHKAI 538 GTGCTC G[AT]GC[AT]^C
666 GAGCTC
BsrI 101 ACTGG ACTGG(1/-1)
641 ACTGG
BstEII 546 GGTTACC G^GT[ACGT]ACC
690 GGTAACC
BstPI 546 GGTTACC G^GT[ACGT]ACC
690 GGTAACC
DpnII 441 GATC ^GATC
645 GATC
EarI 136 GAAGAG CTCTTC(1/4)
229 GAAGAG
EcoO65I 546 GGTTACC G^GT[ACGT]ACC
690 GGTAACC
EcoT14I 398 CCTTGG C^C[AT][AT]GG
458 CCAAGG
HaeII 58 GGCGCT [GA]GCGC^[CT]
145 AGCGCT
HinfI 172 GACTC G^A[ACGT]TC
429 GACTC
Ksp632I 136 GAAGAG CTCTTC(1/4)
229 GAAGAG
MaeI 149 CTAG C^TAG
622 CTAG
MboI 441 GATC ^GATC
645 GATC
MseI 161 TTAA T^TAA
302 TTAA
NdeII 441 GATC ^GATC
645 GATC
PleI 172 GACTC GAGTC(4/5)
429 GACTC
RsaI 45 GTAC GT^AC
447 GTAC
Sau3AI 441 GATC ^GATC
645 GATC
StyI 398 CCTTGG C^C[AT][AT]GG
458 CCAAGG
TaqI 72 TCGA T^CGA
343 TCGA
Tru9I 161 TTAA T^TAA
302 TTAA
Tsp45I 199 GTGAC ^GT[GC]AC
311 GTGAC
Tsp509I 246 AATT ^AATT
617 AATT
TthHB8I 72 TCGA T^CGA
343 TCGA
XcmI 113 CCAGCGTTGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
483 CCAGAGCCTGGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AciI AluI BbvI BcnI BmyI
BsaJI BsiYI BslI Bsp1286I BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI EcoRII Fnu4HI FokI HaeIII
HapII HhaI HinP1I HpaII HphI
ItaI MaeIII MboII MnlI MspI
MvaI MwoI NciI NlaIII Sau96I
ScrFI SfaNI TseI