Restriction map for
pF1KB6493
##################################
# NO CUT ENZYMES ( 175 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AfaI AflII AflIII AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI Asp718I AspEI AspHI AspI
AvaI AviII AvrII BalI BanI
BbeI BbrPI BbvCI BbvI BcgI
BciVI BclI BcnI BfrI BglI
BlnI BlpI Bpu1102I BsaAI BsaHI
BsaI BsaJI BseAI BseRI BsgI
BsiEI BsiHKAI BsiWI BsmAI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BsrI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I CelII CfoI
Cfr10I ClaI CpoI DraI DraIII
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FbaI
Fnu4HI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HincII HindII
HpaI HphI ItaI KasI KpnI
KspI MfeI MluI MluNI MroI
MscI MspA1I MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PshAI
Psp1406I PstI PvuI PvuII RcaI
RsaI RsrII SacI SacII SalI
SapI SbfI ScaI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI Sse8387I SspBI SspI StuI
StyI SwaI TseI TspRI Tth111I
Van91I XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AccI 374 GTCTAC GT^[AC][GT]AC
AlwNI 554 CAGGTTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 674 CCTCAGG CC^T[ACGT]AGG
AvaII 732 GGTCC G^G[AT]CC
BamHI 858 GGATCC G^GATCC
BanII 682 GGGCTC G[GA]GC[CT]^C
BbsI 758 GAAGAC GAAGAC(2/6)
BglII 578 AGATCT A^GATCT
BpmI 401 CTGGAG CTGGAG(16/14)
BpuAI 758 GAAGAC GAAGAC(2/6)
BsaBI 274 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 42 ACCGGA [AT]^CCGG[AT]
BsmFI 180 GGGAC GGGAC(10/14)
Bsu36I 674 CCTCAGG CC^T[ACGT]AGG
Cfr13I 732 GGTCC G^G[ACGT]CC
DraII 731 GGGTCCT [GA]G^G[ACGT]CC[CT]
EarI 205 GAAGAG CTCTTC(1/4)
Eco81I 674 CCTCAGG CC^T[ACGT]AGG
EcoO109I 731 GGGTCCT [GA]G^G[ACGT]CC[CT]
FauI 680 GCGGG CCCGC(4/6)
HindIII 472 AAGCTT A^AGCTT
Ksp632I 205 GAAGAG CTCTTC(1/4)
MaeII 344 ACGT A^CGT
MamI 274 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 238 CATGAATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MwoI 451 GCAACTTTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PpuMI 731 GGGTCCT [GA]G^G[AT]CC[CT]
Sau96I 732 GGTCC G^G[ACGT]CC
SexAI 552 ACCAGGT A^CC[AT]GGT
SfcI 376 CTACAG C^T[GA][CT]AG
SphI 941 GCATGC GCATG^C
TaqI 503 TCGA T^CGA
Tsp45I 894 GTCAC ^GT[GC]AC
TthHB8I 503 TCGA T^CGA
XcmI 234 CCAACATGAATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AciI 445 GCGG CCGC(-3/-1)
680 GCGG
AcsI 505 GAATTT [GA]^AATT[CT]
630 AAATTC
AluI 473 AGCT AG^CT
983 AGCT
ApoI 505 GAATTT [GA]^AATT[CT]
630 AAATTC
AseI 331 ATTAAT AT^TAAT
484 ATTAAT
AsnI 331 ATTAAT AT^TAAT
484 ATTAAT
Asp700I 205 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
952 GAAGTGCTTC
BfaI 260 CTAG C^TAG
985 CTAG
BmyI 3 GGGCAC G[AGT]GC[ACT]^C
682 GGGCTC
Bsp1286I 3 GGGCAC G[AGT]GC[ACT]^C
682 GGGCTC
BstF5I 83 CATCC GGATG(2/0)
18 GGATG
Cac8I 680 GCGGGC GC[ACGT]^[ACGT]GC
941 GCATGC
FokI 83 CATCC GGATG(9/13)
18 GGATG
HaeIII 695 GGCC GG^CC
842 GGCC
HapII 43 CCGG C^CGG
697 CCGG
HpaII 43 CCGG C^CGG
697 CCGG
MaeI 260 CTAG C^TAG
985 CTAG
MaeIII 705 GTTAC ^GT[ACGT]AC
894 GTCAC
MnlI 277 CCTC CCTC(7/6)
674 CCTC
MspI 43 CCGG C^CGG
697 CCGG
NlaIV 731 GGGTCC GG[ACGT]^[ACGT]CC
858 GGATCC
PshBI 331 ATTAAT AT^TAAT
484 ATTAAT
XmnI 205 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
952 GAAGTGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 25 enzymes) #
#########################################
AlwI BsiYI BslI BssKI BstNI
BstYI DdeI DpnII EcoRII HinfI
MboI MboII MflI MseI MvaI
NdeII NlaIII NspI Sau3AI ScrFI
SfaNI TfiI Tru9I Tsp509I XhoII