Restriction map for
pF1KB6804
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# NO CUT ENZYMES ( 195 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AflIII
AgeI AhdI Alw44I AlwI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspHI AvaI AviII
AvrII BalI BamHI BanI BanII
BbeI BbrPI BbsI BbvCI BcgI
BclI BcnI BfrI BglI BglII
BlnI BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BseRI BsgI BsiEI
BsiHKAI BsiWI BsiYI BslI BsmAI
BsmBI BsmI BsoBI Bsp1286I Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstF5I
BstPI BstUI BstXI BstYI BstZ17I
Cac8I CelII CfoI Cfr10I ClaI
CpoI DraI DraIII DrdI DsaI
EagI Eam1105I EarI EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FokI FseI
FspI HaeII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI HpaII HphI KasI KpnI
Ksp632I KspI MamI MfeI MflI
MluI MluNI MroI MscI MslI
MspA1I MspI MunI MvnI MwoI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NlaIV NotI NruI
NsiI NspI NspV PacI PaeR7I
PflMI PinAI PleI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SfaNI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SphI Sse8387I SspBI
SspI StuI SwaI Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AfaI 9 GTAC GT^AC
AlwNI 280 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 285 CCTGAGG CC^T[ACGT]AGG
Asp700I 319 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 478 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvI 546 GCTGC GCAGC(8/12)
BciVI 168 GGATAC GTATCC(6/5)
BsmFI 178 GGGAC GGGAC(10/14)
BsrDI 196 GCAATG GCAATG(2/0)
BsrI 508 CCAGT ACTGG(1/-1)
Bsu36I 285 CCTGAGG CC^T[ACGT]AGG
DpnII 453 GATC ^GATC
DraII 281 AGGACCT [GA]G^G[ACGT]CC[CT]
EaeI 222 CGGCCA [CT]^GGCC[GA]
Eco81I 285 CCTGAGG CC^T[ACGT]AGG
EcoO109I 281 AGGACCT [GA]G^G[ACGT]CC[CT]
Fnu4HI 546 GCTGC GC^[ACGT]GC
ItaI 546 GCTGC GC^[ACGT]GC
MaeII 265 ACGT A^CGT
MboI 453 GATC ^GATC
NdeII 453 GATC ^GATC
NlaIII 539 CATG CATG^
PflFI 478 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 281 AGGACCT [GA]G^G[AT]CC[CT]
PstI 173 CTGCAG CTGCA^G
RsaI 9 GTAC GT^AC
Sau3AI 453 GATC ^GATC
SexAI 479 ACCTGGT A^CC[AT]GGT
SpeI 237 ACTAGT A^CTAGT
TseI 546 GCTGC G^C[AT]GC
Tsp45I 353 GTGAC ^GT[GC]AC
Tth111I 478 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 319 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AcsI 77 GAATTT [GA]^AATT[CT]
396 GAATTT
AluI 14 AGCT AG^CT
255 AGCT
ApoI 77 GAATTT [GA]^AATT[CT]
396 GAATTT
AvaII 21 GGTCC G^G[AT]CC
282 GGACC
BfaI 16 CTAG C^TAG
238 CTAG
BsaJI 24 CCTTGG C^C[ACGT][ACGT]GG
225 CCAAGG
DdeI 257 CTCAG C^T[ACGT]AG
286 CTGAG
EcoT14I 24 CCTTGG C^C[AT][AT]GG
225 CCAAGG
HaeIII 223 GGCC GG^CC
391 GGCC
HinfI 112 GATTC G^A[ACGT]TC
433 GAATC
MaeI 16 CTAG C^TAG
238 CTAG
MaeIII 353 GTGAC ^GT[ACGT]AC
416 GTAAC
SfcI 117 CTACAG C^T[GA][CT]AG
173 CTGCAG
StyI 24 CCTTGG C^C[AT][AT]GG
225 CCAAGG
TaqI 137 TCGA T^CGA
158 TCGA
TfiI 112 GATTC G^A[AT]TC
433 GAATC
TthHB8I 137 TCGA T^CGA
158 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 13 enzymes) #
#########################################
BssKI BstNI Cfr13I EcoRII MboII
MnlI MseI MvaI Sau96I ScrFI
Tru9I Tsp509I TspRI