Restriction map for
pF1KB6818
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# NO CUT ENZYMES ( 177 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaII AviII AvrII BamHI BanI
BanII BbeI BbrPI BbvCI BcgI
BclI BfrI BglII BlnI BlpI
BmyI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BseRI BsiEI
BsiHKAI BsiWI BsmBI BsmFI BsmI
Bsp1286I Bsp1407I BspDI BspEI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstYI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DdeI
DraI DraII DraIII DrdI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FbaI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HinfI
HpaI KasI KpnI Ksp632I KspI
MaeII MamI MfeI MflI MluI
MroI MseI MspA1I MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI
ScaI SfcI SfoI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
TfiI Tru9I Tth111I XbaI XhoI
XhoII XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccII 237 CGCG CG^CG
AcsI 475 AAATTC [GA]^AATT[CT]
ApoI 475 AAATTC [GA]^AATT[CT]
AvaI 31 CCCGGG C^[CT]CG[GA]G
BbsI 201 GAAGAC GAAGAC(2/6)
BciVI 334 GGATAC GTATCC(6/5)
BfaI 11 CTAG C^TAG
BglI 232 GCCATCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 91 CTGGAG CTGGAG(16/14)
BpuAI 201 GAAGAC GAAGAC(2/6)
BsmAI 178 GAGAC GTCTC(1/5)
BsoBI 31 CCCGGG C^[CT]CG[GA]G
BspHI 513 TCATGA T^CATGA
BsrI 111 CCAGT ACTGG(1/-1)
BssSI 149 CACGAG CACGAG(-5/-1)
BstUI 237 CGCG CG^CG
BstXI 219 CCACAGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 324 GCGTGC GC[ACGT]^[ACGT]GC
Cfr13I 230 GGGCC G^G[ACGT]CC
DpnII 366 GATC ^GATC
DsaI 60 CCACGG C^C[GA][CT]GG
HapII 32 CCGG C^CGG
HpaII 32 CCGG C^CGG
MaeI 11 CTAG C^TAG
MaeIII 52 GTGAC ^GT[ACGT]AC
MboI 366 GATC ^GATC
MslI 190 CACTGTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 32 CCGG C^CGG
MvnI 237 CGCG CG^CG
NdeII 366 GATC ^GATC
NlaIV 229 GGGGCC GG[ACGT]^[ACGT]CC
NspI 128 ACATGC [GA]CATG^[CT]
PflMI 111 CCAGTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 513 TCATGA T^CATGA
Sau3AI 366 GATC ^GATC
Sau96I 230 GGGCC G^G[ACGT]CC
SexAI 102 ACCTGGT A^CC[AT]GGT
SfaNI 158 GCATC GCATC(5/9)
SfiI 231 GGCCATCGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 31 CCCGGG CCC^GGG
TaqI 264 TCGA T^CGA
Tsp45I 52 GTGAC ^GT[GC]AC
TthHB8I 264 TCGA T^CGA
Van91I 111 CCAGTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 60 CCACGGCTGGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 31 CCCGGG C^CCGGG
XmaI 31 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AfaI 294 GTAC GT^AC
550 GTAC
AluI 89 AGCT AG^CT
185 AGCT
BalI 216 TGGCCA TGG^CCA
489 TGGCCA
BbvI 165 GCTGC GCAGC(8/12)
186 GCTGC
BcnI 31 CCCGG CC^[GC]GG
32 CCGGG
BsgI 187 CTGCAC GTGCAG(16/14)
391 GTGCAG
BstF5I 48 GGATG GGATG(2/0)
312 GGATG
FauI 344 CCCGC CCCGC(4/6)
44 GCGGG
FokI 48 GGATG GGATG(9/13)
312 GGATG
HphI 78 GGTGA GGTGA(8/7)
341 TCACC
MluNI 216 TGGCCA TGG^CCA
489 TGGCCA
MnlI 41 GAGG CCTC(7/6)
246 GAGG
MscI 216 TGGCCA TGG^CCA
489 TGGCCA
MwoI 65 GCTGGTATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
232 GCCATCGCGGC
NciI 31 CCCGG CC^[GC]GG
32 CCGGG
RsaI 294 GTAC GT^AC
550 GTAC
TseI 165 GCTGC G^C[AT]GC
186 GCTGC
#########################################
# 3 OR MORE CUT ENZYMES ( 17 enzymes) #
#########################################
AciI BsaJI BsiYI BslI BssKI
BstNI EaeI EcoRII Fnu4HI HaeIII
ItaI MboII MvaI NlaIII ScrFI
Tsp509I TspRI