Restriction map for
pF1KB6724
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# NO CUT ENZYMES ( 177 enzymes) #
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Acc65I AccIII AclI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AvrII BalI BamHI BanI
BanII BbeI BbrPI BbsI BbvCI
BcgI BciVI BcnI BfaI BfrI
BglI BglII BlnI BpuAI BsaAI
BsaBI BsaI BsaJI BsaWI BseAI
BsiEI BsiWI BsiYI BslI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrFI
BsrGI BssSI Bst1107I BstAPI BstBI
BstXI BstZ17I Bsu36I Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
DsaI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoRI EcoRV EcoT14I EcoT22I
FauI FseI HaeII HapII HgaI
HindIII HinfI HpaI HpaII KasI
KpnI Ksp632I KspI MaeI MamI
MfeI MluI MluNI MroI MscI
MseI MslI MspA1I MspI MunI
NaeI NarI NciI NcoI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SapI SbfI ScaI SexAI
SfaNI SfcI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI TfiI Tru9I Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AatII 73 GACGTC GACGT^C
AccI 7 GTCGAC GT^[AC][GT]AC
AccII 64 CGCG CG^CG
AcsI 45 GAATTT [GA]^AATT[CT]
AcyI 73 GACGTC G[GA]^CG[CT]C
ApoI 45 GAATTT [GA]^AATT[CT]
AspHI 165 GAGCAC G[AT]GC[AT]^C
AvaII 320 GGACC G^G[AT]CC
AviII 89 TGCGCA TGC^GCA
BbvI 345 GCTGC GCAGC(8/12)
BclI 149 TGATCA T^GATCA
BlpI 162 GCTGAGC GC^T[ACGT]AGC
BmyI 165 GAGCAC G[AGT]GC[ACT]^C
Bpu1102I 162 GCTGAGC GC^T[ACGT]AGC
BsaHI 73 GACGTC G[GA]^CG[CT]C
BsiHKAI 165 GAGCAC G[AT]GC[AT]^C
BsmAI 272 GAGAC GTCTC(1/5)
BsmFI 266 GGGAC GGGAC(10/14)
Bsp1286I 165 GAGCAC G[AGT]GC[ACT]^C
BspMI 59 ACCTGC ACCTGC(4/8)
BssHII 63 GCGCGC G^CGCGC
BstEII 139 GGTGACC G^GT[ACGT]ACC
BstF5I 153 CATCC GGATG(2/0)
BstPI 139 GGTGACC G^GT[ACGT]ACC
BstUI 64 CGCG CG^CG
BstYI 219 GGATCT [GA]^GATC[CT]
CelII 162 GCTGAGC GC^T[ACGT]AGC
Cfr13I 320 GGACC G^G[ACGT]CC
DdeI 163 CTGAG C^T[ACGT]AG
EcoO65I 139 GGTGACC G^GT[ACGT]ACC
FbaI 149 TGATCA T^GATCA
Fnu4HI 345 GCTGC GC^[ACGT]GC
FokI 153 CATCC GGATG(9/13)
FspI 89 TGCGCA TGC^GCA
HaeIII 84 GGCC GG^CC
Hin1I 73 GACGTC G[GA]^CG[CT]C
ItaI 345 GCTGC GC^[ACGT]GC
MaeII 74 ACGT A^CGT
MaeIII 140 GTGAC ^GT[ACGT]AC
MboII 27 GAAGA GAAGA(8/7)
MflI 219 GGATCT [GA]^GATC[CT]
MvnI 64 CGCG CG^CG
MwoI 158 GCACGCTGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeI 145 CATATG CA^TATG
NlaIV 320 GGACCC GG[ACGT]^[ACGT]CC
SalI 7 GTCGAC G^TCGAC
Sau96I 320 GGACC G^G[ACGT]CC
TseI 345 GCTGC G^C[AT]GC
Tsp45I 140 GTGAC ^GT[GC]AC
Tsp509I 46 AATT ^AATT
XhoII 219 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 14 enzymes) #
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AciI 156 CCGC CCGC(-3/-1)
240 CCGC
AfaI 21 GTAC GT^AC
57 GTAC
AlwI 219 GGATC GGATC(4/5)
329 GGATC
BpmI 280 CTCCAG CTGGAG(16/14)
352 CTGGAG
BseRI 52 GAGGAG GAGGAG(10/8)
214 GAGGAG
BssKI 195 CCAGG ^CC[ACGT]GG
351 CCTGG
BstNI 195 CCAGG CC^[AT]GG
351 CCTGG
EcoRII 195 CCAGG ^CC[AT]GG
351 CCTGG
HincII 7 GTCGAC GT[CT]^[GA]AC
37 GTCAAC
HindII 7 GTCGAC GT[CT]^[GA]AC
37 GTCAAC
MvaI 195 CCAGG CC^[AT]GG
351 CCTGG
NlaIII 186 CATG CATG^
249 CATG
RsaI 21 GTAC GT^AC
57 GTAC
ScrFI 195 CCAGG CC^[ACGT]GG
351 CCTGG
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# 3 OR MORE CUT ENZYMES ( 16 enzymes) #
#########################################
AluI BsgI BsrI Cac8I CfoI
DpnII HhaI HinP1I HphI MboI
MnlI NdeII Sau3AI TaqI TspRI
TthHB8I