##################################
# NO CUT ENZYMES ( 178 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AfaI AflII
AflIII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AvaII AviII
AvrII BalI BbeI BbrPI BbsI
BcgI BciVI BclI BfaI BfrI
BglI BlnI BlpI BpmI Bpu1102I
BpuAI BsaAI BsaHI BsaI BsaWI
BseAI BseRI BsiEI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstUI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DraI DraII DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoRI EcoRV EcoT22I
FauI FbaI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HinfI HpaI KasI
KpnI KspI MaeI MfeI MluI
MluNI MroI MscI MseI MunI
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsaI RsrII
SacI SacII SalI SbfI ScaI
SfcI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TfiI Tru9I
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AluI 292 AGCT AG^CT
AspHI 235 GTGCTC G[AT]GC[AT]^C
BanI 334 GGCACC G^G[CT][GA]CC
BbvCI 247 GCTGAGG CCTCAGC(-5/-2)
BcnI 427 CCCGG CC^[GC]GG
BglII 192 AGATCT A^GATCT
BsaBI 187 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 104 CTGCAC GTGCAG(16/14)
BsiHKAI 235 GTGCTC G[AT]GC[AT]^C
BsmAI 63 GTCTC GTCTC(1/5)
BsmFI 119 GTCCC GGGAC(10/14)
BsrI 23 CCAGT ACTGG(1/-1)
BstEII 497 GGTTACC G^GT[ACGT]ACC
BstF5I 35 GGATG GGATG(2/0)
BstPI 497 GGTTACC G^GT[ACGT]ACC
BstXI 172 CCAAAATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DsaI 11 CCATGG C^C[GA][CT]GG
EaeI 429 CGGCCA [CT]^GGCC[GA]
EarI 276 GAAGAG CTCTTC(1/4)
EcoO65I 497 GGTTACC G^GT[ACGT]ACC
FokI 35 GGATG GGATG(9/13)
HapII 428 CCGG C^CGG
HpaII 428 CCGG C^CGG
Ksp632I 276 GAAGAG CTCTTC(1/4)
MaeII 302 ACGT A^CGT
MamI 187 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 148 CACTGCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 114 CCGCTG C[AC]G^C[GT]G
MspI 428 CCGG C^CGG
NciI 427 CCCGG CC^[GC]GG
NcoI 11 CCATGG C^CATGG
SapI 276 GAAGAGC GCTCTTC(1/4)
SexAI 224 ACCTGGT A^CC[AT]GGT
SmlI 420 CTCAAG C^T[CT][GA]AG
StuI 134 AGGCCT AGG^CCT
TaqI 448 TCGA T^CGA
Tsp45I 168 GTCAC ^GT[GC]AC
Tsp509I 385 AATT ^AATT
TthHB8I 448 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 11 enzymes) #
###################################
AciI 114 CCGC CCGC(-3/-1)
210 GCGG
BamHI 110 GGATCC G^GATCC
253 GGATCC
BanII 313 GGGCTC G[GA]GC[CT]^C
340 GAGCCC
Cac8I 199 GCCTGC GC[ACGT]^[ACGT]GC
439 GCGAGC
Cfr13I 15 GGCCC G^G[ACGT]CC
212 GGCCC
EcoT14I 11 CCATGG C^C[AT][AT]GG
131 CCAAGG
MboII 79 GAAGA GAAGA(8/7)
276 GAAGA
MwoI 143 GCTCCCACTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
190 GCAGATCTGGC
Sau96I 15 GGCCC G^G[ACGT]CC
212 GGCCC
SfaNI 36 GATGC GCATC(5/9)
187 GATGC
StyI 11 CCATGG C^C[AT][AT]GG
131 CCAAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
#########################################
AlwI BbvI BmyI BsaJI BsiYI
BslI Bsp1286I BssKI BstNI BstYI
DdeI DpnII EcoRII Fnu4HI HaeIII
HphI ItaI MaeIII MboI MflI
MnlI MvaI NdeII NlaIII NlaIV
Sau3AI ScrFI TseI TspRI XhoII