Restriction map for pF1KB6845
##################################
# NO CUT ENZYMES (  178 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AfaI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwNI       
AocI        Aor51HI     ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AvaI        AvaII       AviII       
AvrII       BalI        BbeI        BbrPI       BbsI        
BcgI        BciVI       BclI        BfaI        BfrI        
BglI        BlnI        BlpI        BpmI        Bpu1102I    
BpuAI       BsaAI       BsaHI       BsaI        BsaWI       
BseAI       BseRI       BsiEI       BsiWI       BsmBI       
BsmI        BsoBI       Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BsrDI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstUI       BstZ17I     Bsu36I      
CelII       CfoI        Cfr10I      ClaI        CpoI        
DraI        DraII       DraIII      DrdI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO109I    EcoRI       EcoRV       EcoT22I     
FauI        FbaI        FseI        FspI        HaeII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HindIII     HinfI       HpaI        KasI        
KpnI        KspI        MaeI        MfeI        MluI        
MluNI       MroI        MscI        MseI        MunI        
MvnI        NaeI        NarI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        PvuII       RcaI        RsaI        RsrII       
SacI        SacII       SalI        SbfI        ScaI        
SfcI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        SwaI        TfiI        Tru9I       
Tth111I     Van91I      XbaI        XcmI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AluI          292  AGCT                 AG^CT
AspHI         235  GTGCTC               G[AT]GC[AT]^C
BanI          334  GGCACC               G^G[CT][GA]CC
BbvCI         247  GCTGAGG              CCTCAGC(-5/-2)
BcnI          427  CCCGG                CC^[GC]GG
BglII         192  AGATCT               A^GATCT
BsaBI         187  GATGCAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI          104  CTGCAC               GTGCAG(16/14)
BsiHKAI       235  GTGCTC               G[AT]GC[AT]^C
BsmAI          63  GTCTC                GTCTC(1/5)
BsmFI         119  GTCCC                GGGAC(10/14)
BsrI           23  CCAGT                ACTGG(1/-1)
BstEII        497  GGTTACC              G^GT[ACGT]ACC
BstF5I         35  GGATG                GGATG(2/0)
BstPI         497  GGTTACC              G^GT[ACGT]ACC
BstXI         172  CCAAAATCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DsaI           11  CCATGG               C^C[GA][CT]GG
EaeI          429  CGGCCA               [CT]^GGCC[GA]
EarI          276  GAAGAG               CTCTTC(1/4)
EcoO65I       497  GGTTACC              G^GT[ACGT]ACC
FokI           35  GGATG                GGATG(9/13)
HapII         428  CCGG                 C^CGG
HpaII         428  CCGG                 C^CGG
Ksp632I       276  GAAGAG               CTCTTC(1/4)
MaeII         302  ACGT                 A^CGT
MamI          187  GATGCAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI          148  CACTGCTATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        114  CCGCTG               C[AC]G^C[GT]G
MspI          428  CCGG                 C^CGG
NciI          427  CCCGG                CC^[GC]GG
NcoI           11  CCATGG               C^CATGG
SapI          276  GAAGAGC              GCTCTTC(1/4)
SexAI         224  ACCTGGT              A^CC[AT]GGT
SmlI          420  CTCAAG               C^T[CT][GA]AG
StuI          134  AGGCCT               AGG^CCT
TaqI          448  TCGA                 T^CGA
Tsp45I        168  GTCAC                ^GT[GC]AC
Tsp509I       385  AATT                 ^AATT
TthHB8I       448  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   11 enzymes) #
###################################

AciI          114  CCGC                 CCGC(-3/-1)
              210  GCGG                
BamHI         110  GGATCC               G^GATCC
              253  GGATCC              
BanII         313  GGGCTC               G[GA]GC[CT]^C
              340  GAGCCC              
Cac8I         199  GCCTGC               GC[ACGT]^[ACGT]GC
              439  GCGAGC              
Cfr13I         15  GGCCC                G^G[ACGT]CC
              212  GGCCC               
EcoT14I        11  CCATGG               C^C[AT][AT]GG
              131  CCAAGG              
MboII          79  GAAGA                GAAGA(8/7)
              276  GAAGA               
MwoI          143  GCTCCCACTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              190  GCAGATCTGGC         
Sau96I         15  GGCCC                G^G[ACGT]CC
              212  GGCCC               
SfaNI          36  GATGC                GCATC(5/9)
              187  GATGC               
StyI           11  CCATGG               C^C[AT][AT]GG
              131  CCAAGG              


#########################################
# 3 OR MORE CUT ENZYMES (   30 enzymes) #
#########################################

AlwI        BbvI        BmyI        BsaJI       BsiYI       
BslI        Bsp1286I    BssKI       BstNI       BstYI       
DdeI        DpnII       EcoRII      Fnu4HI      HaeIII      
HphI        ItaI        MaeIII      MboI        MflI        
MnlI        MvaI        NdeII       NlaIII      NlaIV       
Sau3AI      ScrFI       TseI        TspRI       XhoII       
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