################################## # NO CUT ENZYMES ( 178 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AvaII AviII AvrII BalI BbeI BbrPI BbsI BcgI BciVI BclI BfaI BfrI BglI BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaI KasI KpnI KspI MaeI MfeI MluI MluNI MroI MscI MseI MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SbfI ScaI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TfiI Tru9I Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AluI 292 AGCT AG^CT AspHI 235 GTGCTC G[AT]GC[AT]^C BanI 334 GGCACC G^G[CT][GA]CC BbvCI 247 GCTGAGG CCTCAGC(-5/-2) BcnI 427 CCCGG CC^[GC]GG BglII 192 AGATCT A^GATCT BsaBI 187 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsgI 104 CTGCAC GTGCAG(16/14) BsiHKAI 235 GTGCTC G[AT]GC[AT]^C BsmAI 63 GTCTC GTCTC(1/5) BsmFI 119 GTCCC GGGAC(10/14) BsrI 23 CCAGT ACTGG(1/-1) BstEII 497 GGTTACC G^GT[ACGT]ACC BstF5I 35 GGATG GGATG(2/0) BstPI 497 GGTTACC G^GT[ACGT]ACC BstXI 172 CCAAAATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DsaI 11 CCATGG C^C[GA][CT]GG EaeI 429 CGGCCA [CT]^GGCC[GA] EarI 276 GAAGAG CTCTTC(1/4) EcoO65I 497 GGTTACC G^GT[ACGT]ACC FokI 35 GGATG GGATG(9/13) HapII 428 CCGG C^CGG HpaII 428 CCGG C^CGG Ksp632I 276 GAAGAG CTCTTC(1/4) MaeII 302 ACGT A^CGT MamI 187 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MslI 148 CACTGCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 114 CCGCTG C[AC]G^C[GT]G MspI 428 CCGG C^CGG NciI 427 CCCGG CC^[GC]GG NcoI 11 CCATGG C^CATGG SapI 276 GAAGAGC GCTCTTC(1/4) SexAI 224 ACCTGGT A^CC[AT]GGT SmlI 420 CTCAAG C^T[CT][GA]AG StuI 134 AGGCCT AGG^CCT TaqI 448 TCGA T^CGA Tsp45I 168 GTCAC ^GT[GC]AC Tsp509I 385 AATT ^AATT TthHB8I 448 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 11 enzymes) # ################################### AciI 114 CCGC CCGC(-3/-1) 210 GCGG BamHI 110 GGATCC G^GATCC 253 GGATCC BanII 313 GGGCTC G[GA]GC[CT]^C 340 GAGCCC Cac8I 199 GCCTGC GC[ACGT]^[ACGT]GC 439 GCGAGC Cfr13I 15 GGCCC G^G[ACGT]CC 212 GGCCC EcoT14I 11 CCATGG C^C[AT][AT]GG 131 CCAAGG MboII 79 GAAGA GAAGA(8/7) 276 GAAGA MwoI 143 GCTCCCACTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 190 GCAGATCTGGC Sau96I 15 GGCCC G^G[ACGT]CC 212 GGCCC SfaNI 36 GATGC GCATC(5/9) 187 GATGC StyI 11 CCATGG C^C[AT][AT]GG 131 CCAAGG ######################################### # 3 OR MORE CUT ENZYMES ( 30 enzymes) # ######################################### AlwI BbvI BmyI BsaJI BsiYI BslI Bsp1286I BssKI BstNI BstYI DdeI DpnII EcoRII Fnu4HI HaeIII HphI ItaI MaeIII MboI MflI MnlI MvaI NdeII NlaIII NlaIV Sau3AI ScrFI TseI TspRI XhoII