Restriction map for
pF1KB8913
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# NO CUT ENZYMES ( 149 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BalI BamHI BbeI BbrPI BcgI
BciVI BfaI BfrI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaWI BseAI BsiWI BsmBI BsmFI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrGI BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstYI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraII DraIII Eam1105I Eco47III
Eco81I EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FseI FspI HgaI Hin1I
HincII HindII HindIII HinfI HpaI
KasI KpnI KspI MaeI MaeII
MamI MflI MluI MluNI MroI
MscI MseI MslI NarI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacI SacII SalI
SbfI ScaI SexAI SfaNI SfcI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI TfiI Tru9I Tth111I Van91I
XbaI XcmI XhoI XhoII
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccI 592 GTAGAC GT^[AC][GT]AC
AfaI 626 GTAC GT^AC
AlwNI 356 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 538 GTGCTC G[AT]GC[AT]^C
AvaII 746 GGTCC G^G[AT]CC
BanII 40 GAGCCC G[GA]GC[CT]^C
BbsI 466 GAAGAC GAAGAC(2/6)
BbvCI 55 GCTGAGG CCTCAGC(-5/-2)
BclI 173 TGATCA T^GATCA
BglI 46 GCCCCGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 466 GAAGAC GAAGAC(2/6)
BsaI 412 GGTCTC GGTCTC(1/5)
BsgI 244 GTGCAG GTGCAG(16/14)
BsiEI 26 CGGCCG CG[GA][CT]^CG
BsiHKAI 538 GTGCTC G[AT]GC[AT]^C
BsmI 455 GAATGC GAATGC(1/-1)
BspMI 441 GCAGGT ACCTGC(4/8)
BssHII 133 GCGCGC G^CGCGC
BstF5I 631 CATCC GGATG(2/0)
DrdI 595 GACCTCTATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 26 CGGCCG C^GGCCG
EarI 666 GAAGAG CTCTTC(1/4)
EclXI 26 CGGCCG C^GGCCG
Eco52I 26 CGGCCG C^GGCCG
EcoNI 606 CCTGGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 43 CCCGC CCCGC(4/6)
FbaI 173 TGATCA T^GATCA
FokI 631 CATCC GGATG(9/13)
Ksp632I 666 GAAGAG CTCTTC(1/4)
MaeIII 358 GTGAC ^GT[ACGT]AC
MfeI 434 CAATTG C^AATTG
MunI 434 CAATTG C^AATTG
NaeI 24 GCCGGC GCC^GGC
NcoI 749 CCATGG C^CATGG
NgoMIV 24 GCCGGC G^CCGGC
NspI 500 ACATGC [GA]CATG^[CT]
PvuII 526 CAGCTG CAG^CTG
RsaI 626 GTAC GT^AC
SapI 666 GAAGAGC GCTCTTC(1/4)
SmlI 96 CTTGAG C^T[CT][GA]AG
StuI 614 AGGCCT AGG^CCT
Tsp45I 358 GTGAC ^GT[GC]AC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
Asp700I 612 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
655 GAAGCCTTTC
AvaI 74 CCCGGG C^[CT]CG[GA]G
254 CCCGGG
BanI 124 GGTGCC G^G[CT][GA]CC
203 GGCACC
BbvI 70 GCAGC GCAGC(8/12)
86 GCAGC
BmyI 40 GAGCCC G[AGT]GC[ACT]^C
538 GTGCTC
BsmAI 391 GAGAC GTCTC(1/5)
413 GTCTC
BsoBI 74 CCCGGG C^[CT]CG[GA]G
254 CCCGGG
Bsp1286I 40 GAGCCC G[AGT]GC[ACT]^C
538 GTGCTC
BsrBI 79 GAGCGG CCGCTC(-3/-3)
99 GAGCGG
BsrFI 24 GCCGGC [GA]^CCGG[CT]
200 ACCGGC
BsrI 295 CCAGT ACTGG(1/-1)
700 CCAGT
Cfr10I 24 GCCGGC [GA]^CCGG[CT]
200 ACCGGC
DpnII 174 GATC ^GATC
484 GATC
DsaI 144 CCGTGG C^C[GA][CT]GG
749 CCATGG
EcoT14I 191 CCAAGG C^C[AT][AT]GG
749 CCATGG
HaeII 210 GGCGCT [GA]GCGC^[CT]
258 GGCGCT
MboI 174 GATC ^GATC
484 GATC
MspA1I 267 CCGCTG C[AC]G^C[GT]G
526 CAGCTG
NdeII 174 GATC ^GATC
484 GATC
Sau3AI 174 GATC ^GATC
484 GATC
SmaI 74 CCCGGG CCC^GGG
254 CCCGGG
StyI 191 CCAAGG C^C[AT][AT]GG
749 CCATGG
TaqI 366 TCGA T^CGA
453 TCGA
TseI 70 GCAGC G^C[AT]GC
86 GCAGC
TthHB8I 366 TCGA T^CGA
453 TCGA
XmaCI 74 CCCGGG C^CCGGG
254 CCCGGG
XmaI 74 CCCGGG C^CCGGG
254 CCCGGG
XmnI 612 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
655 GAAGCCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AccII AciI AluI BcnI BpmI
BsaJI BseRI BsiYI BslI BssKI
BstNI BstUI Cac8I CfoI Cfr13I
DdeI EaeI EcoRII Fnu4HI HaeIII
HapII HhaI HinP1I HpaII HphI
ItaI MboII MnlI MspI MvaI
MvnI MwoI NciI NlaIII NlaIV
Sau96I ScrFI Tsp509I TspRI