Restriction map for
pF1KB8920
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# NO CUT ENZYMES ( 136 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AlwI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BamHI BbrPI BbvCI
BciVI BclI BfaI BfrI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaI BsaWI BseAI BseRI BsgI
BsiWI BsmI Bsp1407I BspDI BspEI
BspLU11I BsrDI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstXI
BstYI BstZ17I CelII ClaI CpoI
DraI DraIII DrdI Eam1105I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KpnI KspI MaeI MamI
MfeI MflI MluI MroI MseI
MunI NaeI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI PvuII RsrII SacI
SacII SalI SbfI ScaI SexAI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TaqI Tru9I Tsp509I Tth111I TthHB8I
Van91I XbaI XcmI XhoI XhoII
XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AatII 316 GACGTC GACGT^C
AluI 536 AGCT AG^CT
AlwNI 202 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 349 CCTGAGG CC^T[ACGT]AGG
BalI 543 TGGCCA TGG^CCA
BbsI 331 GAAGAC GAAGAC(2/6)
BcgI 573 CGACGGCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 569 GCCCCGACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 331 GAAGAC GAAGAC(2/6)
BsmBI 318 CGTCTC CGTCTC(1/5)
BsmFI 251 GGGAC GGGAC(10/14)
BspHI 719 TCATGA T^CATGA
BspMI 125 ACCTGC ACCTGC(4/8)
BsrBI 21 CCGCTC CCGCTC(-3/-3)
BsrFI 444 GCCGGT [GA]^CCGG[CT]
BstAPI 618 GCACAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 349 CCTGAGG CC^T[ACGT]AGG
Cfr10I 444 GCCGGT [GA]^CCGG[CT]
DpnII 8 GATC ^GATC
EarI 732 GAAGAG CTCTTC(1/4)
Eco81I 349 CCTGAGG CC^T[ACGT]AGG
Ksp632I 732 GAAGAG CTCTTC(1/4)
MaeIII 751 GTGAC ^GT[ACGT]AC
MboI 8 GATC ^GATC
MluNI 543 TGGCCA TGG^CCA
MscI 543 TGGCCA TGG^CCA
MslI 63 CATGGAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeII 8 GATC ^GATC
PpuMI 104 AGGACCT [GA]G^G[AT]CC[CT]
PstI 15 CTGCAG CTGCA^G
RcaI 719 TCATGA T^CATGA
SapI 732 GAAGAGC GCTCTTC(1/4)
Sau3AI 8 GATC ^GATC
SfaNI 137 GCATC GCATC(5/9)
SfiI 568 GGCCCCGACGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 183 CCCGGG CCC^GGG
TfiI 257 GATTC G^A[AT]TC
Tsp45I 751 GTGAC ^GT[GC]AC
XmaCI 183 CCCGGG C^CCGGG
XmaI 183 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AfaI 228 GTAC GT^AC
477 GTAC
Aor51HI 407 AGCGCT AGC^GCT
704 AGCGCT
AvaII 105 GGACC G^G[AT]CC
252 GGACC
BbeI 461 GGCGCC GGCGC^C
487 GGCGCC
BsmAI 169 GAGAC GTCTC(1/5)
319 GTCTC
BstF5I 138 CATCC GGATG(2/0)
665 GGATG
DdeI 321 CTCAG C^T[ACGT]AG
350 CTGAG
Eco47III 407 AGCGCT AGC^GCT
704 AGCGCT
FokI 138 CATCC GGATG(9/13)
665 GGATG
HgaI 741 GACGC GACGC(5/10)
753 GACGC
HphI 750 GGTGA GGTGA(8/7)
110 TCACC
KasI 461 GGCGCC G^GCGCC
487 GGCGCC
MaeII 230 ACGT A^CGT
317 ACGT
NarI 461 GGCGCC GG^CGCC
487 GGCGCC
NcoI 62 CCATGG C^CATGG
419 CCATGG
RsaI 228 GTAC GT^AC
477 GTAC
SfcI 15 CTGCAG C^T[GA][CT]AG
660 CTACAG
SfoI 461 GGCGCC GGC^GCC
487 GGCGCC
StuI 56 AGGCCT AGG^CCT
509 AGGCCT
TspRI 97 CAGTG CA[GC]TG[ACGT][ACGT]^
467 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AccII AciI AcyI ApaI AspHI
AvaI BanI BanII BbvI BcnI
BmyI BpmI BsaHI BsaJI BsiEI
BsiHKAI BsiYI BslI BsoBI Bsp1286I
BsrI BssKI BstNI BstUI Cac8I
CfoI Cfr13I DraII DsaI EaeI
EagI EclXI Eco52I EcoO109I EcoRII
EcoT14I FauI Fnu4HI HaeII HaeIII
HapII HhaI Hin1I HinP1I HinfI
HpaII ItaI MboII MnlI MspA1I
MspI MvaI MvnI MwoI NciI
NlaIII NlaIV PleI Sau96I ScrFI
StyI TseI