Restriction map for
pF1KB7680
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# NO CUT ENZYMES ( 122 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvrII BalI BbrPI BbvCI BciVI
BclI BfrI BlnI BlpI Bpu1102I
BsaAI BsaBI BsaI BseAI BsiWI
BsmAI BsmBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrGI BssHII Bst1107I
BstBI BstUI BstZ17I CelII ClaI
CpoI DraI DraIII Eam1105I EarI
Eco47III EcoNI EcoRI EcoRV EcoT22I
FbaI FseI HincII HindII HindIII
HpaI KpnI Ksp632I KspI MamI
MfeI MluI MluNI MroI MscI
MseI MspA1I MunI MvnI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacII SalI SapI SbfI
SexAI SfcI SfiI SfuI SnaBI
SpeI Sse8387I SspBI SspI StuI
SwaI Tru9I Tth111I Van91I XbaI
XcmI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AcsI 1159 AAATTC [GA]^AATT[CT]
AocI 1033 CCTGAGG CC^T[ACGT]AGG
ApoI 1159 AAATTC [GA]^AATT[CT]
AviII 902 TGCGCA TGC^GCA
BamHI 1209 GGATCC G^GATCC
BbeI 964 GGCGCC GGCGC^C
BbvI 60 GCTGC GCAGC(8/12)
BcgI 905 GCATTGCCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 335 CTAG C^TAG
BglII 326 AGATCT A^GATCT
BsiEI 472 CGGCCG CG[GA][CT]^CG
BspMI 256 ACCTGC ACCTGC(4/8)
BsrDI 906 CATTGC GCAATG(2/0)
BstAPI 441 GCAGGCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 697 GGTGACC G^GT[ACGT]ACC
BstPI 697 GGTGACC G^GT[ACGT]ACC
BstXI 887 CCAAGTACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1033 CCTGAGG CC^T[ACGT]AGG
DrdI 211 GACTGCATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 609 CCGTGG C^C[GA][CT]GG
EagI 472 CGGCCG C^GGCCG
EclXI 472 CGGCCG C^GGCCG
Eco52I 472 CGGCCG C^GGCCG
Eco81I 1033 CCTGAGG CC^T[ACGT]AGG
EcoO65I 697 GGTGACC G^GT[ACGT]ACC
FspI 902 TGCGCA TGC^GCA
HaeII 964 GGCGCC [GA]GCGC^[CT]
KasI 964 GGCGCC G^GCGCC
MaeI 335 CTAG C^TAG
MslI 498 CACAACGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 9 GCCGGC GCC^GGC
NarI 964 GGCGCC GG^CGCC
NgoMIV 9 GCCGGC G^CCGGC
PaeR7I 913 CTCGAG C^TCGAG
PpuMI 981 AGGACCT [GA]G^G[AT]CC[CT]
ScaI 890 AGTACT AGT^ACT
SfaNI 310 GATGC GCATC(5/9)
SfoI 964 GGCGCC GGC^GCC
SgrAI 8 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 526 CCCGGG CCC^GGG
SmlI 913 CTCGAG C^T[CT][GA]AG
SphI 437 GCATGC GCATG^C
TaqI 914 TCGA T^CGA
TfiI 200 GATTC G^A[AT]TC
TseI 60 GCTGC G^C[AT]GC
TthHB8I 914 TCGA T^CGA
XhoI 913 CTCGAG C^TCGAG
XmaCI 526 CCCGGG C^CCGGG
XmaI 526 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AfaI 891 GTAC GT^AC
1080 GTAC
AlwNI 198 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG
587 CAGGCCCTG
AvaI 526 CCCGGG C^[CT]CG[GA]G
913 CTCGAG
BbsI 688 GAAGAC GAAGAC(2/6)
852 GAAGAC
BglI 945 GCCAGGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1055 GCCTGGAGGGC
BpuAI 688 GAAGAC GAAGAC(2/6)
852 GAAGAC
BsaWI 65 ACCGGA [AT]^CCGG[AT]
976 ACCGGA
BsgI 61 CTGCAC GTGCAG(16/14)
391 CTGCAC
BsoBI 526 CCCGGG C^[CT]CG[GA]G
913 CTCGAG
BsrBI 234 GAGCGG CCGCTC(-3/-3)
916 GAGCGG
BsrFI 9 GCCGGC [GA]^CCGG[CT]
679 GCCGGT
BssSI 790 CTCGTG CACGAG(-5/-1)
814 CTCGTG
BstF5I 90 GGATG GGATG(2/0)
543 GGATG
BstYI 326 AGATCT [GA]^GATC[CT]
1209 GGATCC
Cfr10I 9 GCCGGC [GA]^CCGG[CT]
679 GCCGGT
EcoT14I 182 CCAAGG C^C[AT][AT]GG
1205 CCAAGG
FokI 90 GGATG GGATG(9/13)
543 GGATG
HgaI 6 GACGC GACGC(5/10)
1064 GCGTC
MaeII 37 ACGT A^CGT
1120 ACGT
MflI 326 AGATCT [GA]^GATC[CT]
1209 GGATCC
NspI 158 ACATGC [GA]CATG^[CT]
437 GCATGC
RsaI 891 GTAC GT^AC
1080 GTAC
StyI 182 CCAAGG C^C[AT][AT]GG
1205 CCAAGG
Tsp509I 783 AATT ^AATT
1160 AATT
XhoII 326 AGATCT [GA]^GATC[CT]
1209 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AciI AcyI AluI AlwI ApaI
AspHI AvaII BanI BanII BcnI
BmyI BpmI BsaHI BsaJI BseRI
BsiHKAI BsiYI BslI BsmFI BsmI
Bsp1286I BsrI BssKI BstNI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EaeI EcoO109I EcoRII FauI Fnu4HI
HaeIII HapII HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI MaeIII
MboI MboII MnlI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI SacI Sau3AI Sau96I ScrFI
Tsp45I TspRI