Restriction map for
pF1KE0019
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# NO CUT ENZYMES ( 144 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI AlwNI AocI Aor51HI AscI
Asp718I AspEI AspI AvaI AviII
AvrII BamHI BanI BbeI BbrPI
BcgI BciVI BcnI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaWI BseAI BsiEI
BsiWI BsmBI BsmFI BsmI BsoBI
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrFI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FauI
FseI FspI HaeII HgaI Hin1I
HincII HindII HpaI KasI KpnI
KspI MamI MfeI MluI MroI
MunI MvnI NaeI NarI NciI
NcoI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
StyI SwaI TaqI Tth111I TthHB8I
Van91I XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AciI 1584 CCGC CCGC(-3/-1)
AcsI 925 GAATTT [GA]^AATT[CT]
Alw44I 1628 GTGCAC G^TGCAC
ApaI 272 GGGCCC GGGCC^C
ApaLI 1628 GTGCAC G^TGCAC
ApoI 925 GAATTT [GA]^AATT[CT]
AseI 1102 ATTAAT AT^TAAT
AsnI 1102 ATTAAT AT^TAAT
Asp700I 193 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 999 GGACC G^G[AT]CC
BbsI 1255 GAAGAC GAAGAC(2/6)
BbvCI 1007 CCTCAGC CCTCAGC(-5/-2)
BclI 1697 TGATCA T^GATCA
BpuAI 1255 GAAGAC GAAGAC(2/6)
Bsp1407I 336 TGTACA T^GTACA
BsrGI 336 TGTACA T^GTACA
BstAPI 1400 GCACTCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 693 GGATCT [GA]^GATC[CT]
CfoI 656 GCGC GCG^C
DraI 495 TTTAAA TTT^AAA
DraII 271 GGGGCCC [GA]G^G[ACGT]CC[CT]
DraIII 1622 CACACGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1668 CCGTGG C^C[GA][CT]GG
EarI 1045 GAAGAG CTCTTC(1/4)
EcoO109I 271 GGGGCCC [GA]G^G[ACGT]CC[CT]
FbaI 1697 TGATCA T^GATCA
HhaI 656 GCGC GCG^C
HinP1I 656 GCGC G^CGC
HindIII 1509 AAGCTT A^AGCTT
HphI 1695 GGTGA GGTGA(8/7)
Ksp632I 1045 GAAGAG CTCTTC(1/4)
MaeII 1310 ACGT A^CGT
MflI 693 GGATCT [GA]^GATC[CT]
NdeI 1222 CATATG CA^TATG
PshBI 1102 ATTAAT AT^TAAT
PstI 1551 CTGCAG CTGCA^G
SapI 1045 GAAGAGC GCTCTTC(1/4)
SmlI 115 CTTGAG C^T[CT][GA]AG
SphI 1022 GCATGC GCATG^C
SspBI 336 TGTACA T^GTACA
StuI 1031 AGGCCT AGG^CCT
XhoII 693 GGATCT [GA]^GATC[CT]
XmnI 193 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AfaI 337 GTAC GT^AC
1247 GTAC
AspHI 1048 GAGCAC G[AT]GC[AT]^C
1628 GTGCAC
BalI 321 TGGCCA TGG^CCA
1379 TGGCCA
BanII 272 GGGCCC G[GA]GC[CT]^C
1360 GAGCCC
BpmI 1598 CTCCAG CTGGAG(16/14)
550 CTGGAG
BsaI 393 GAGACC GGTCTC(1/5)
901 GAGACC
BsaJI 522 CCCTGG C^C[ACGT][ACGT]GG
1668 CCGTGG
BseRI 917 GAGGAG GAGGAG(10/8)
920 GAGGAG
BsiHKAI 1048 GAGCAC G[AT]GC[AT]^C
1628 GTGCAC
BsmAI 393 GAGAC GTCTC(1/5)
901 GAGAC
BspMI 1001 ACCTGC ACCTGC(4/8)
1646 ACCTGC
EaeI 321 TGGCCA [CT]^GGCC[GA]
1379 TGGCCA
HapII 354 CCGG C^CGG
1027 CCGG
HpaII 354 CCGG C^CGG
1027 CCGG
MluNI 321 TGGCCA TGG^CCA
1379 TGGCCA
MscI 321 TGGCCA TGG^CCA
1379 TGGCCA
MslI 181 CACATGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1686 CATGGATATG
MspA1I 332 CAGCTG C[AC]G^C[GT]G
1010 CAGCTG
MspI 354 CCGG C^CGG
1027 CCGG
NspI 1022 GCATGC [GA]CATG^[CT]
1590 ACATGC
PleI 1374 GACTC GAGTC(4/5)
1436 GAGTC
PvuII 332 CAGCTG CAG^CTG
1010 CAGCTG
RsaI 337 GTAC GT^AC
1247 GTAC
SfcI 1180 CTACAG C^T[GA][CT]AG
1551 CTGCAG
SspI 163 AATATT AAT^ATT
1490 AATATT
XbaI 939 TCTAGA T^CTAGA
1432 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AluI AlwI BbvI BfaI BmyI
BsgI BsiYI BslI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII EcoRII Fnu4HI FokI
HaeIII HinfI ItaI MaeI MaeIII
MboI MboII MnlI MseI MvaI
MwoI NdeII NlaIII NlaIV Sau3AI
Sau96I ScrFI SfaNI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XcmI