Restriction map for
pF1KE0519
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# NO CUT ENZYMES ( 169 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AfaI AflII
AflIII AgeI AhdI Alw44I AlwI
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AvaI AviII AvrII BamHI
BanI BbeI BbrPI BbvCI BcgI
BciVI BcnI BfaI BfrI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaWI BseAI BsiEI BsiHKAI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstXI
BstYI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DraIII DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FauI FseI FspI HgaI
Hin1I HincII HindII HindIII HinfI
HpaI KasI KpnI KspI MaeI
MaeII MamI MfeI MflI MluI
MroI MslI MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PinAI PleI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI RcaI RsaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TfiI XbaI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AlwNI 514 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
AspI 256 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 300 GGACC G^G[AT]CC
BalI 162 TGGCCA TGG^CCA
BbsI 312 GAAGAC GAAGAC(2/6)
BclI 551 TGATCA T^GATCA
BglI 155 GCCTGTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 312 GAAGAC GAAGAC(2/6)
BsaI 202 GAGACC GGTCTC(1/5)
BsgI 47 GTGCAG GTGCAG(16/14)
BsrBI 87 GAGCGG CCGCTC(-3/-3)
BsrDI 71 GCAATG GCAATG(2/0)
BstAPI 116 GCAACATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CfoI 77 GCGC GCG^C
DdeI 35 CTGAG C^T[ACGT]AG
DpnII 552 GATC ^GATC
DrdI 218 GACAGGTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT14I 137 CCAAGG C^C[AT][AT]GG
FbaI 551 TGATCA T^GATCA
HaeII 76 GGCGCT [GA]GCGC^[CT]
HapII 349 CCGG C^CGG
HhaI 77 GCGC GCG^C
HinP1I 77 GCGC G^CGC
HpaII 349 CCGG C^CGG
HphI 549 GGTGA GGTGA(8/7)
MaeIII 522 GTCAC ^GT[ACGT]AC
MboI 552 GATC ^GATC
MluNI 162 TGGCCA TGG^CCA
MscI 162 TGGCCA TGG^CCA
MseI 452 TTAA T^TAA
MspA1I 479 CAGCTG C[AC]G^C[GT]G
MspI 349 CCGG C^CGG
NdeII 552 GATC ^GATC
NlaIII 555 CATG CATG^
PflFI 256 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 498 CCAGTATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 299 AGGACCC [GA]G^G[AT]CC[CT]
PvuII 479 CAGCTG CAG^CTG
Sau3AI 552 GATC ^GATC
SmlI 329 CTCAAG C^T[CT][GA]AG
StyI 137 CCAAGG C^C[AT][AT]GG
TaqI 110 TCGA T^CGA
Tru9I 452 TTAA T^TAA
Tsp45I 522 GTCAC ^GT[GC]AC
Tsp509I 414 AATT ^AATT
Tth111I 256 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 110 TCGA T^CGA
Van91I 498 CCAGTATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 399 CCAGATGTCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 22 enzymes) #
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AciI 89 GCGG CCGC(-3/-1)
468 GCGG
AluI 240 AGCT AG^CT
480 AGCT
ApaI 13 GGGCCC GGGCC^C
55 GGGCCC
BanII 13 GGGCCC G[GA]GC[CT]^C
55 GGGCCC
BbvI 68 GCAGC GCAGC(8/12)
284 GCAGC
BmyI 13 GGGCCC G[AGT]GC[ACT]^C
55 GGGCCC
BpmI 42 CTCCAG CTGGAG(16/14)
476 CTCCAG
BseRI 320 CTCCTC GAGGAG(10/8)
473 CTCCTC
BsmAI 202 GAGAC GTCTC(1/5)
254 GAGAC
BsmFI 490 GGGAC GGGAC(10/14)
484 GTCCC
Bsp1286I 13 GGGCCC G[AGT]GC[ACT]^C
55 GGGCCC
BssKI 487 CCTGG ^CC[ACGT]GG
546 CCTGG
BstF5I 264 CATCC GGATG(2/0)
141 GGATG
BstNI 487 CCTGG CC^[AT]GG
546 CCTGG
EaeI 162 TGGCCA [CT]^GGCC[GA]
178 TGGCCG
EarI 169 CTCTTC CTCTTC(1/4)
529 CTCTTC
EcoRII 487 CCTGG ^CC[AT]GG
546 CCTGG
FokI 264 CATCC GGATG(9/13)
141 GGATG
Ksp632I 169 CTCTTC CTCTTC(1/4)
529 CTCTTC
MvaI 487 CCTGG CC^[AT]GG
546 CCTGG
ScrFI 487 CCTGG CC^[ACGT]GG
546 CCTGG
TseI 68 GCAGC G^C[AT]GC
284 GCAGC
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# 3 OR MORE CUT ENZYMES ( 18 enzymes) #
#########################################
BsaJI BsiYI BslI BsrI Cac8I
Cfr13I DraII EcoO109I Fnu4HI HaeIII
ItaI MboII MnlI MwoI NlaIV
Sau96I SfaNI TspRI