################################## # NO CUT ENZYMES ( 191 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BbvI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaHI BsaI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsiYI BslI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1286I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I CelII Cfr13I ClaI CpoI DraII DraIII DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI Fnu4HI FseI FspI HaeII HgaI Hin1I HincII HindII HindIII HpaI HphI ItaI KasI KpnI KspI MaeII MfeI MflI MluI MluNI MroI MscI MslI MspA1I MunI NarI NciI NcoI NdeI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI Sau96I SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspI StuI SwaI TseI Tsp45I TspRI Tth111I Van91I XbaI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AccII 29 CGCG CG^CG AciI 221 CCGC CCGC(-3/-1) AfaI 242 GTAC GT^AC Asp700I 94 GAAAAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BfaI 49 CTAG C^TAG BsaBI 268 GATAGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 109 ACCGGA [AT]^CCGG[AT] Bsp1407I 241 TGTACA T^GTACA BsrFI 40 GCCGGC [GA]^CCGG[CT] BsrGI 241 TGTACA T^GTACA BsrI 255 CCAGT ACTGG(1/-1) BssKI 23 CCAGG ^CC[ACGT]GG BstNI 23 CCAGG CC^[AT]GG BstUI 29 CGCG CG^CG CfoI 30 GCGC GCG^C Cfr10I 40 GCCGGC [GA]^CCGG[CT] DdeI 329 CTCAG C^T[ACGT]AG DpnII 283 GATC ^GATC DraI 259 TTTAAA TTT^AAA DrdI 64 GACAAGCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 212 GAAGAG CTCTTC(1/4) EcoRII 23 CCAGG ^CC[AT]GG EcoT14I 224 CCAAGG C^C[AT][AT]GG HaeIII 228 GGCC GG^CC HhaI 30 GCGC GCG^C HinP1I 30 GCGC G^CGC HinfI 99 GATTC G^A[ACGT]TC Ksp632I 212 GAAGAG CTCTTC(1/4) MaeI 49 CTAG C^TAG MaeIII 217 GTAAC ^GT[ACGT]AC MamI 268 GATAGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MboI 283 GATC ^GATC MseI 260 TTAA T^TAA MvaI 23 CCAGG CC^[AT]GG MvnI 29 CGCG CG^CG MwoI 21 GCCCAGGTCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NaeI 40 GCCGGC GCC^GGC NdeII 283 GATC ^GATC NgoMIV 40 GCCGGC G^CCGGC NheI 48 GCTAGC G^CTAGC NspI 44 GCATGC [GA]CATG^[CT] RsaI 242 GTAC GT^AC Sau3AI 283 GATC ^GATC ScrFI 23 CCAGG CC^[ACGT]GG SfaNI 32 GCATC GCATC(5/9) SphI 44 GCATGC GCATG^C SspBI 241 TGTACA T^GTACA StyI 224 CCAAGG C^C[AT][AT]GG TfiI 99 GATTC G^A[AT]TC Tru9I 260 TTAA T^TAA XcmI 155 CCATCGAGTTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 94 GAAAAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 11 enzymes) # ################################### AluI 51 AGCT AG^CT 303 AGCT BsaJI 22 CCCAGG C^C[ACGT][ACGT]GG 224 CCAAGG BstF5I 103 CATCC GGATG(2/0) 274 CATCC FokI 103 CATCC GGATG(9/13) 274 CATCC HapII 41 CCGG C^CGG 110 CCGG HpaII 41 CCGG C^CGG 110 CCGG MnlI 328 CCTC CCTC(7/6) 87 GAGG MspI 41 CCGG C^CGG 110 CCGG NlaIV 151 GGAACC GG[ACGT]^[ACGT]CC 168 GGAACC TaqI 62 TCGA T^CGA 158 TCGA TthHB8I 62 TCGA T^CGA 158 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 4 enzymes) # ######################################### Cac8I MboII NlaIII Tsp509I