Restriction map for pF1KB6783
##################################
# NO CUT ENZYMES (  191 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwI        AlwNI       AocI        
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp718I     AspEI       AspHI       
AspI        AvaI        AvaII       AviII       AvrII       
BalI        BamHI       BanI        BanII       BbeI        
BbrPI       BbsI        BbvCI       BbvI        BcgI        
BciVI       BclI        BcnI        BfrI        BglI        
BglII       BlnI        BlpI        BmyI        BpmI        
Bpu1102I    BpuAI       BsaAI       BsaHI       BsaI        
BseAI       BseRI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsiYI       BslI        BsmAI       BsmBI       
BsmFI       BsmI        BsoBI       Bsp1286I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrDI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstXI       BstYI       
BstZ17I     Bsu36I      CelII       Cfr13I      ClaI        
CpoI        DraII       DraIII      DsaI        EaeI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO109I    EcoO65I     EcoRI       
EcoRV       EcoT22I     FauI        FbaI        Fnu4HI      
FseI        FspI        HaeII       HgaI        Hin1I       
HincII      HindII      HindIII     HpaI        HphI        
ItaI        KasI        KpnI        KspI        MaeII       
MfeI        MflI        MluI        MluNI       MroI        
MscI        MslI        MspA1I      MunI        NarI        
NciI        NcoI        NdeI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PleI        PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PstI        PvuI        PvuII       RcaI        RsrII       
SacI        SacII       SalI        SapI        Sau96I      
SbfI        ScaI        SexAI       SfcI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        Sse8387I    SspI        StuI        
SwaI        TseI        Tsp45I      TspRI       Tth111I     
Van91I      XbaI        XhoI        XhoII       XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

AccII          29  CGCG                 CG^CG
AciI          221  CCGC                 CCGC(-3/-1)
AfaI          242  GTAC                 GT^AC
Asp700I        94  GAAAAGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BfaI           49  CTAG                 C^TAG
BsaBI         268  GATAGCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI         109  ACCGGA               [AT]^CCGG[AT]
Bsp1407I      241  TGTACA               T^GTACA
BsrFI          40  GCCGGC               [GA]^CCGG[CT]
BsrGI         241  TGTACA               T^GTACA
BsrI          255  CCAGT                ACTGG(1/-1)
BssKI          23  CCAGG                ^CC[ACGT]GG
BstNI          23  CCAGG                CC^[AT]GG
BstUI          29  CGCG                 CG^CG
CfoI           30  GCGC                 GCG^C
Cfr10I         40  GCCGGC               [GA]^CCGG[CT]
DdeI          329  CTCAG                C^T[ACGT]AG
DpnII         283  GATC                 ^GATC
DraI          259  TTTAAA               TTT^AAA
DrdI           64  GACAAGCCTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI          212  GAAGAG               CTCTTC(1/4)
EcoRII         23  CCAGG                ^CC[AT]GG
EcoT14I       224  CCAAGG               C^C[AT][AT]GG
HaeIII        228  GGCC                 GG^CC
HhaI           30  GCGC                 GCG^C
HinP1I         30  GCGC                 G^CGC
HinfI          99  GATTC                G^A[ACGT]TC
Ksp632I       212  GAAGAG               CTCTTC(1/4)
MaeI           49  CTAG                 C^TAG
MaeIII        217  GTAAC                ^GT[ACGT]AC
MamI          268  GATAGCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI          283  GATC                 ^GATC
MseI          260  TTAA                 T^TAA
MvaI           23  CCAGG                CC^[AT]GG
MvnI           29  CGCG                 CG^CG
MwoI           21  GCCCAGGTCGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NaeI           40  GCCGGC               GCC^GGC
NdeII         283  GATC                 ^GATC
NgoMIV         40  GCCGGC               G^CCGGC
NheI           48  GCTAGC               G^CTAGC
NspI           44  GCATGC               [GA]CATG^[CT]
RsaI          242  GTAC                 GT^AC
Sau3AI        283  GATC                 ^GATC
ScrFI          23  CCAGG                CC^[ACGT]GG
SfaNI          32  GCATC                GCATC(5/9)
SphI           44  GCATGC               GCATG^C
SspBI         241  TGTACA               T^GTACA
StyI          224  CCAAGG               C^C[AT][AT]GG
TfiI           99  GATTC                G^A[AT]TC
Tru9I         260  TTAA                 T^TAA
XcmI          155  CCATCGAGTTGATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI           94  GAAAAGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   11 enzymes) #
###################################

AluI           51  AGCT                 AG^CT
              303  AGCT                
BsaJI          22  CCCAGG               C^C[ACGT][ACGT]GG
              224  CCAAGG              
BstF5I        103  CATCC                GGATG(2/0)
              274  CATCC               
FokI          103  CATCC                GGATG(9/13)
              274  CATCC               
HapII          41  CCGG                 C^CGG
              110  CCGG                
HpaII          41  CCGG                 C^CGG
              110  CCGG                
MnlI          328  CCTC                 CCTC(7/6)
               87  GAGG                
MspI           41  CCGG                 C^CGG
              110  CCGG                
NlaIV         151  GGAACC               GG[ACGT]^[ACGT]CC
              168  GGAACC              
TaqI           62  TCGA                 T^CGA
              158  TCGA                
TthHB8I        62  TCGA                 T^CGA
              158  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (    4 enzymes) #
#########################################

Cac8I       MboII       NlaIII      Tsp509I     
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