Restriction map for
pF1KE0134
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# NO CUT ENZYMES ( 168 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AhdI
Alw44I AlwNI AocI Aor51HI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaI AvaII
AvrII BamHI BbeI BbrPI BbvCI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BlpI Bpu1102I BsaAI
BsaHI BsaI BseAI BseRI BsiEI
BsiHKAI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
CelII ClaI CpoI DdeI DraI
DrdI DsaI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FbaI FseI HaeII HgaI Hin1I
HincII HindII HindIII HpaI KasI
KpnI Ksp632I KspI MaeI MfeI
MluI MroI MseI MslI MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI Sse8387I SspBI SspI StuI
StyI SwaI TaqI Tru9I Tsp45I
Tth111I TthHB8I Van91I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AgeI 26 ACCGGT A^CCGGT
AlwI 492 GATCC GGATC(4/5)
ApaI 629 GGGCCC GGGCC^C
AviII 455 TGCGCA TGC^GCA
BanI 349 GGTGCC G^G[CT][GA]CC
BanII 629 GGGCCC G[GA]GC[CT]^C
BbsI 544 GAAGAC GAAGAC(2/6)
BcnI 261 CCGGG CC^[GC]GG
BglI 190 GCCCTGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 193 CTGGAG CTGGAG(16/14)
BpuAI 544 GAAGAC GAAGAC(2/6)
BsaBI 492 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 26 ACCGGT [AT]^CCGG[AT]
BsgI 634 CTGCAC GTGCAG(16/14)
BsmAI 57 GTCTC GTCTC(1/5)
BsmFI 76 GTCCC GGGAC(10/14)
BsrFI 26 ACCGGT [GA]^CCGG[CT]
BsrI 602 CCAGT ACTGG(1/-1)
BstYI 491 AGATCC [GA]^GATC[CT]
CfoI 456 GCGC GCG^C
Cfr10I 26 ACCGGT [GA]^CCGG[CT]
DpnII 492 GATC ^GATC
DraIII 151 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
FspI 455 TGCGCA TGC^GCA
HhaI 456 GCGC GCG^C
HinP1I 456 GCGC G^CGC
HinfI 90 GATTC G^A[ACGT]TC
HphI 518 TCACC GGTGA(8/7)
MaeII 284 ACGT A^CGT
MaeIII 23 GTTAC ^GT[ACGT]AC
MamI 492 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 492 GATC ^GATC
MflI 491 AGATCC [GA]^GATC[CT]
MspA1I 175 CAGCTG C[AC]G^C[GT]G
NciI 261 CCGGG CC^[GC]GG
NdeII 492 GATC ^GATC
PinAI 26 ACCGGT A^CCGGT
PstI 424 CTGCAG CTGCA^G
PvuII 175 CAGCTG CAG^CTG
Sau3AI 492 GATC ^GATC
SfcI 424 CTGCAG C^T[GA][CT]AG
SphI 341 GCATGC GCATG^C
TfiI 90 GATTC G^A[AT]TC
TspRI 372 CACTG CA[GC]TG[ACGT][ACGT]^
XcmI 215 CCAATAAGTACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 491 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 16 enzymes) #
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AcsI 206 GAATTT [GA]^AATT[CT]
538 GAATTT
AluI 176 AGCT AG^CT
422 AGCT
ApoI 206 GAATTT [GA]^AATT[CT]
538 GAATTT
BmyI 369 GGGCAC G[AGT]GC[ACT]^C
629 GGGCCC
Bsp1286I 369 GGGCAC G[AGT]GC[ACT]^C
629 GGGCCC
Cac8I 341 GCATGC GC[ACGT]^[ACGT]GC
627 GCGGGC
DraII 449 AGGCCCT [GA]G^G[ACGT]CC[CT]
629 GGGCCCT
EcoO109I 449 AGGCCCT [GA]G^G[ACGT]CC[CT]
629 GGGCCCT
FauI 13 CCCGC CCCGC(4/6)
627 GCGGG
HapII 27 CCGG C^CGG
261 CCGG
HpaII 27 CCGG C^CGG
261 CCGG
MboII 489 GAAGA GAAGA(8/7)
544 GAAGA
MspI 27 CCGG C^CGG
261 CCGG
NspI 341 GCATGC [GA]CATG^[CT]
438 GCATGT
SfaNI 82 GATGC GCATC(5/9)
293 GCATC
Tsp509I 207 AATT ^AATT
539 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
#########################################
AciI AfaI BalI BbvI BsaJI
BsiYI BslI BssKI BstF5I BstNI
Cfr13I EaeI EcoRII Fnu4HI FokI
HaeIII ItaI MluNI MnlI MscI
MvaI MwoI NlaIII NlaIV RsaI
Sau96I ScrFI TseI