Restriction map for
pF1KB6728
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# NO CUT ENZYMES ( 180 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AvrII BalI BamHI BanI
BbeI BbrPI BbsI BbvCI BciVI
BcnI BfaI BfrI BglI BglII
BlnI BpuAI BsaAI BsaBI BsaHI
BsaI BsaJI BsaWI BseAI BseRI
BsgI BsiEI BsiWI BsmAI BsmBI
BsmFI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrGI BssHII
BssKI BssSI Bst1107I BstAPI BstBI
BstEII BstNI BstPI BstXI BstZ17I
Bsu36I ClaI CpoI DraI DraII
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRII EcoRV
EcoT14I EcoT22I FseI HgaI Hin1I
HincII HindII HindIII HpaI KasI
KpnI KspI MaeI MaeII MaeIII
MamI MfeI MluI MluNI MroI
MscI MslI MunI MvaI NarI
NciI NcoI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacII SalI SapI
SbfI ScaI ScrFI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI TaqI
Tsp45I Tth111I TthHB8I Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccII 259 CGCG CG^CG
AfaI 303 GTAC GT^AC
AluI 406 AGCT AG^CT
AspHI 405 GAGCTC G[AT]GC[AT]^C
BcgI 170 GCAAAGGTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 50 TGATCA T^GATCA
BlpI 267 GCTCAGC GC^T[ACGT]AGC
BpmI 39 CTCCAG CTGGAG(16/14)
Bpu1102I 267 GCTCAGC GC^T[ACGT]AGC
BsiHKAI 405 GAGCTC G[AT]GC[AT]^C
BsmI 252 GAATGC GAATGC(1/-1)
BspMI 314 ACCTGC ACCTGC(4/8)
BsrDI 15 GCAATG GCAATG(2/0)
BsrFI 156 GCCGGC [GA]^CCGG[CT]
BsrI 41 CCAGT ACTGG(1/-1)
BstUI 259 CGCG CG^CG
BstYI 199 AGATCC [GA]^GATC[CT]
CelII 267 GCTCAGC GC^T[ACGT]AGC
Cfr10I 156 GCCGGC [GA]^CCGG[CT]
EaeI 204 CGGCCA [CT]^GGCC[GA]
EarI 378 GAAGAG CTCTTC(1/4)
FbaI 50 TGATCA T^GATCA
HaeII 343 AGCGCC [GA]GCGC^[CT]
HinfI 129 GATTC G^A[ACGT]TC
Ksp632I 378 GAAGAG CTCTTC(1/4)
MboII 378 GAAGA GAAGA(8/7)
MflI 199 AGATCC [GA]^GATC[CT]
MseI 191 TTAA T^TAA
MspA1I 208 CAGCGG C[AC]G^C[GT]G
MvnI 259 CGCG CG^CG
MwoI 335 GCAGGGCCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NaeI 156 GCCGGC GCC^GGC
NgoMIV 156 GCCGGC G^CCGGC
RsaI 303 GTAC GT^AC
SacI 405 GAGCTC GAGCT^C
SfcI 72 CTACAG C^T[GA][CT]AG
TfiI 129 GATTC G^A[AT]TC
Tru9I 191 TTAA T^TAA
Tsp509I 106 AATT ^AATT
TspRI 78 CACTG CA[GC]TG[ACGT][ACGT]^
XhoII 199 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AlwI 200 GATCC GGATC(4/5)
288 GATCC
AvaII 236 GGACC G^G[AT]CC
389 GGACC
BanII 82 GAGCCC G[GA]GC[CT]^C
405 GAGCTC
BmyI 82 GAGCCC G[AGT]GC[ACT]^C
405 GAGCTC
Bsp1286I 82 GAGCCC G[AGT]GC[ACT]^C
405 GAGCTC
DdeI 80 CTGAG C^T[ACGT]AG
268 CTCAG
FauI 101 CCCGC CCCGC(4/6)
392 CCCGC
HapII 157 CCGG C^CGG
203 CCGG
HpaII 157 CCGG C^CGG
203 CCGG
HphI 375 GGTGA GGTGA(8/7)
132 TCACC
MspI 157 CCGG C^CGG
203 CCGG
NlaIII 285 CATG CATG^
321 CATG
NlaIV 153 GGAGCC GG[ACGT]^[ACGT]CC
389 GGACCC
SfaNI 9 GCATC GCATC(5/9)
275 GCATC
#########################################
# 3 OR MORE CUT ENZYMES ( 23 enzymes) #
#########################################
AciI AviII BbvI BsiYI BslI
BstF5I Cac8I CfoI Cfr13I DpnII
Fnu4HI FokI FspI HaeIII HhaI
HinP1I ItaI MboI MnlI NdeII
Sau3AI Sau96I TseI