################################## # NO CUT ENZYMES ( 165 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflIII AgeI AhdI AlwNI Aor51HI ApaI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BamHI BanI BbeI BbsI BbvCI BciVI BclI BcnI BfaI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstUI BstXI BstZ17I CelII ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FokI FseI FspI HaeII HgaI Hin1I HincII HindII HpaI HphI KasI KpnI Ksp632I KspI MaeI MaeIII MfeI MluI MroI MspA1I MunI MvnI NarI NciI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmeI PpuMI PshAI PshBI Psp1406I PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tsp45I Tsp509I TspRI Tth111I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AciI 270 CCGC CCGC(-3/-1) AfaI 449 GTAC GT^AC AflII 94 CTTAAG C^TTAAG Alw44I 197 GTGCAC G^TGCAC AocI 117 CCTGAGG CC^T[ACGT]AGG ApaLI 197 GTGCAC G^TGCAC AvaII 105 GGACC G^G[AT]CC BalI 2 TGGCCA TGG^CCA BanII 343 GGGCTC G[GA]GC[CT]^C BbrPI 200 CACGTG CAC^GTG BcgI 279 CGATCGACTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfrI 94 CTTAAG C^TTAAG BsaAI 200 CACGTG [CT]AC^GT[GA] BsaBI 83 GATACGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiEI 279 CGATCG CG[GA][CT]^CG BsmFI 36 GTCCC GGGAC(10/14) BsrDI 294 CATTGC GCAATG(2/0) BsrFI 356 GCCGGC [GA]^CCGG[CT] BsrI 33 CCAGT ACTGG(1/-1) BssSI 323 CTCGTG CACGAG(-5/-1) BstYI 251 AGATCC [GA]^GATC[CT] Bsu36I 117 CCTGAGG CC^T[ACGT]AGG CfoI 129 GCGC GCG^C Cfr10I 356 GCCGGC [GA]^CCGG[CT] Eco81I 117 CCTGAGG CC^T[ACGT]AGG HapII 357 CCGG C^CGG HhaI 129 GCGC GCG^C HinP1I 129 GCGC G^CGC HindIII 307 AAGCTT A^AGCTT HinfI 88 GAATC G^A[ACGT]TC HpaII 357 CCGG C^CGG MamI 83 GATACGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MboII 165 GAAGA GAAGA(8/7) MflI 251 AGATCC [GA]^GATC[CT] MluNI 2 TGGCCA TGG^CCA MscI 2 TGGCCA TGG^CCA MslI 49 CACAGAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 357 CCGG C^CGG NaeI 356 GCCGGC GCC^GGC NgoMIV 356 GCCGGC G^CCGGC NspI 125 ACATGC [GA]CATG^[CT] PmaCI 200 CACGTG CAC^GTG PmlI 200 CACGTG CAC^GTG PstI 410 CTGCAG CTGCA^G PvuI 279 CGATCG CGAT^CG RsaI 449 GTAC GT^AC SfaNI 168 GATGC GCATC(5/9) SfcI 410 CTGCAG C^T[GA][CT]AG SmlI 94 CTTAAG C^T[CT][GA]AG TfiI 88 GAATC G^A[AT]TC XcmI 300 CCACCCCAAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 251 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AlwI 252 GATCC GGATC(4/5) 390 GGATC AspHI 197 GTGCAC G[AT]GC[AT]^C 320 GTGCTC BbvI 412 GCAGC GCAGC(8/12) 409 GCTGC BsiHKAI 197 GTGCAC G[AT]GC[AT]^C 320 GTGCTC BssKI 405 CCAGG ^CC[ACGT]GG 453 CCAGG BstNI 405 CCAGG CC^[AT]GG 453 CCAGG EaeI 2 TGGCCA [CT]^GGCC[GA] 358 CGGCCA EcoRII 405 CCAGG ^CC[AT]GG 453 CCAGG MaeII 201 ACGT A^CGT 447 ACGT MseI 95 TTAA T^TAA 242 TTAA MvaI 405 CCAGG CC^[AT]GG 453 CCAGG NlaIII 126 CATG CATG^ 192 CATG ScrFI 405 CCAGG CC^[ACGT]GG 453 CCAGG TaqI 278 TCGA T^CGA 282 TCGA Tru9I 95 TTAA T^TAA 242 TTAA TseI 409 GCTGC G^C[AT]GC 412 GCAGC TthHB8I 278 TCGA T^CGA 282 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 24 enzymes) # ######################################### AluI BmyI BsaJI BsiYI BslI Bsp1286I Cac8I Cfr13I DdeI DpnII DraII EcoO109I EcoT14I Fnu4HI HaeIII ItaI MboI MnlI MwoI NdeII NlaIV Sau3AI Sau96I StyI