##################################
# NO CUT ENZYMES (  215 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcsI        AcyI        AfaI        
AflII       AflIII      AgeI        AhdI        AluI        
Alw44I      AlwNI       AocI        Aor51HI     ApaI        
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspHI       AspI        
AvaI        AvaII       AviII       AvrII       BalI        
BamHI       BanI        BbeI        BbrPI       BbsI        
BbvCI       BcgI        BciVI       BclI        BcnI        
BfaI        BfrI        BglI        BglII       BlnI        
BlpI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BsiEI       
BsiHKAI     BsiWI       BsmAI       BsmBI       BsmFI       
BsmI        BsoBI       Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BsrDI       
BsrFI       BsrGI       BsrI        BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstUI       BstXI       BstZ17I     Bsu36I      CelII       
CfoI        Cfr10I      Cfr13I      ClaI        CpoI        
DdeI        DraI        DraII       DraIII      DrdI        
EaeI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoO109I    EcoO65I     EcoRI       
EcoRV       EcoT14I     EcoT22I     FauI        FbaI        
FseI        FspI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HindIII     HinfI       HpaI        HpaII       
KasI        KpnI        KspI        MaeI        MaeII       
MaeIII      MamI        MfeI        MluI        MluNI       
MroI        MscI        MseI        MspA1I      MspI        
MunI        MvnI        NaeI        NarI        NciI        
NcoI        NdeI        NgoMIV      NheI        NlaIV       
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PleI        PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PstI        PvuI        
PvuII       RcaI        RsaI        RsrII       SacI        
SacII       SalI        SapI        Sau96I      SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
StyI        SwaI        TaqI        TfiI        Tru9I       
Tsp45I      Tth111I     TthHB8I     Van91I      XbaI        
XcmI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   23 enzymes) #
###################################

AlwI           15  GGATC                GGATC(4/5)
BanII          78  GAGCCC               G[GA]GC[CT]^C
BmyI           78  GAGCCC               G[AGT]GC[ACT]^C
BpmI           32  CTCCAG               CTGGAG(16/14)
BseRI          40  GAGGAG               GAGGAG(10/8)
BsgI           73  GTGCAG               GTGCAG(16/14)
Bsp1286I       78  GAGCCC               G[AGT]GC[ACT]^C
BstYI          15  GGATCT               [GA]^GATC[CT]
Cac8I         191  GCCAGC               GC[ACGT]^[ACGT]GC
DpnII          16  GATC                 ^GATC
DsaI          131  CCACGG               C^C[GA][CT]GG
HphI          128  TCACC                GGTGA(8/7)
MboI           16  GATC                 ^GATC
MflI           15  GGATCT               [GA]^GATC[CT]
MslI           96  CATGGATGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MwoI          110  GCCCAGGATGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeII          16  GATC                 ^GATC
Sau3AI         16  GATC                 ^GATC
SfaNI         116  GATGC                GCATC(5/9)
SfcI          197  CTACAG               C^T[GA][CT]AG
Tsp509I        85  AATT                 ^AATT
TspRI         180  CACTG                CA[GC]TG[ACGT][ACGT]^
XhoII          15  GGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

BbvI          246  GCAGC                GCAGC(8/12)
              173  GCTGC               
BsaJI         111  CCCAGG               C^C[ACGT][ACGT]GG
              131  CCACGG              
BsiYI         196  CCTACAGGAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              233  CCTTCAGGAGG         
BslI          196  CCTACAGGAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              233  CCTTCAGGAGG         
BssKI          69  CCTGG                ^CC[ACGT]GG
              112  CCAGG               
BstNI          69  CCTGG                CC^[AT]GG
              112  CCAGG               
EarI           37  GAAGAG               CTCTTC(1/4)
               45  GAAGAG              
EcoNI         196  CCTACAGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              233  CCTTCAGGAGG         
EcoRII         69  CCTGG                ^CC[AT]GG
              112  CCAGG               
Fnu4HI        173  GCTGC                GC^[ACGT]GC
              246  GCAGC               
ItaI          173  GCTGC                GC^[ACGT]GC
              246  GCAGC               
Ksp632I        37  GAAGAG               CTCTTC(1/4)
               45  GAAGAG              
MvaI           69  CCTGG                CC^[AT]GG
              112  CCAGG               
NlaIII         96  CATG                 CATG^
              145  CATG                
ScrFI          69  CCTGG                CC^[ACGT]GG
              112  CCAGG               
TseI          173  GCTGC                G^C[AT]GC
              246  GCAGC               


#########################################
# 3 OR MORE CUT ENZYMES (    4 enzymes) #
#########################################

BstF5I      FokI        MboII       MnlI