Restriction map for
pF1KB6856
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# NO CUT ENZYMES ( 169 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
AlwI AlwNI AocI Aor51HI ApaI
AscI AseI AsnI Asp718I AspEI
AvaI AviII AvrII BalI BamHI
BanI BanII BbeI BbrPI BbvCI
BcgI BciVI BclI BcnI BfrI
BglI BglII BlnI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsgI
BsiEI BsiWI BsiYI BslI BsmAI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrGI BsrI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstYI BstZ17I Bsu36I ClaI
CpoI DraI DraII DraIII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRV EcoT14I EcoT22I FauI
FbaI FseI FspI HaeIII Hin1I
HincII HindII HindIII HpaI HphI
KasI KpnI KspI MaeII MaeIII
MamI MflI MluI MluNI MroI
MscI MslI NarI NciI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI StuI StyI
SwaI Tsp45I Van91I XbaI XcmI
XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AciI 432 CCGC CCGC(-3/-1)
Alw44I 373 GTGCAC G^TGCAC
ApaLI 373 GTGCAC G^TGCAC
AspHI 373 GTGCAC G[AT]GC[AT]^C
AspI 394 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 102 GGACC G^G[AT]CC
BbsI 400 GTCTTC GAAGAC(2/6)
BlpI 112 GCTCAGC GC^T[ACGT]AGC
BmyI 373 GTGCAC G[AGT]GC[ACT]^C
Bpu1102I 112 GCTCAGC GC^T[ACGT]AGC
BpuAI 400 GTCTTC GAAGAC(2/6)
BseRI 291 GAGGAG GAGGAG(10/8)
BsiHKAI 373 GTGCAC G[AT]GC[AT]^C
BsmFI 347 GTCCC GGGAC(10/14)
Bsp1286I 373 GTGCAC G[AGT]GC[ACT]^C
BsrFI 452 GCCGGC [GA]^CCGG[CT]
Cac8I 452 GCCGGC GC[ACGT]^[ACGT]GC
CelII 112 GCTCAGC GC^T[ACGT]AGC
CfoI 14 GCGC GCG^C
Cfr10I 452 GCCGGC [GA]^CCGG[CT]
Cfr13I 102 GGACC G^G[ACGT]CC
DpnII 21 GATC ^GATC
EarI 77 CTCTTC CTCTTC(1/4)
HaeII 13 AGCGCC [GA]GCGC^[CT]
HhaI 14 GCGC GCG^C
HinP1I 14 GCGC G^CGC
Ksp632I 77 CTCTTC CTCTTC(1/4)
MboI 21 GATC ^GATC
MfeI 577 CAATTG C^AATTG
MseI 554 TTAA T^TAA
MspA1I 368 CAGCTG C[AC]G^C[GT]G
MunI 577 CAATTG C^AATTG
MwoI 117 GCTCAGGGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NaeI 452 GCCGGC GCC^GGC
NdeII 21 GATC ^GATC
NgoMIV 452 GCCGGC G^CCGGC
NlaIII 271 CATG CATG^
NlaIV 353 GGAGCC GG[ACGT]^[ACGT]CC
NspI 270 GCATGT [GA]CATG^[CT]
PflFI 394 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 58 GAGTC GAGTC(4/5)
PvuII 368 CAGCTG CAG^CTG
SapI 76 GCTCTTC GCTCTTC(1/4)
Sau3AI 21 GATC ^GATC
Sau96I 102 GGACC G^G[ACGT]CC
SspI 130 AATATT AAT^ATT
TfiI 428 GAATC G^A[AT]TC
Tru9I 554 TTAA T^TAA
TspRI 261 CAGTG CA[GC]TG[ACGT][ACGT]^
Tth111I 394 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 28 enzymes) #
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AccII 241 CGCG CG^CG
433 CGCG
AcsI 296 GAATTC [GA]^AATT[CT]
562 GAATTC
ApoI 296 GAATTC [GA]^AATT[CT]
562 GAATTC
Asp700I 207 GAAATCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
252 GAAAAAGTTC
BbvI 139 GCAGC GCAGC(8/12)
449 GCAGC
BfaI 235 CTAG C^TAG
442 CTAG
BpmI 82 CTGGAG CTGGAG(16/14)
88 CTGGAG
BsaJI 71 CCCAGG C^C[ACGT][ACGT]GG
349 CCCAGG
BssKI 72 CCAGG ^CC[ACGT]GG
350 CCAGG
BstF5I 384 CATCC GGATG(2/0)
549 GGATG
BstNI 72 CCAGG CC^[AT]GG
350 CCAGG
BstUI 241 CGCG CG^CG
433 CGCG
EcoRI 296 GAATTC G^AATTC
562 GAATTC
EcoRII 72 CCAGG ^CC[AT]GG
350 CCAGG
Fnu4HI 139 GCAGC GC^[ACGT]GC
449 GCAGC
FokI 384 CATCC GGATG(9/13)
549 GGATG
HgaI 345 GCGTC GACGC(5/10)
434 GCGTC
HinfI 58 GAGTC G^A[ACGT]TC
428 GAATC
ItaI 139 GCAGC GC^[ACGT]GC
449 GCAGC
MaeI 235 CTAG C^TAG
442 CTAG
MboII 78 TCTTC GAAGA(8/7)
401 TCTTC
MvaI 72 CCAGG CC^[AT]GG
350 CCAGG
MvnI 241 CGCG CG^CG
433 CGCG
ScrFI 72 CCAGG CC^[ACGT]GG
350 CCAGG
SfaNI 379 GATGC GCATC(5/9)
65 GCATC
SmlI 106 CTCAAG C^T[CT][GA]AG
388 CTTGAG
TseI 139 GCAGC G^C[AT]GC
449 GCAGC
XmnI 207 GAAATCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
252 GAAAAAGTTC
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# 3 OR MORE CUT ENZYMES ( 11 enzymes) #
#########################################
AfaI AluI DdeI HapII HpaII
MnlI MspI RsaI TaqI Tsp509I
TthHB8I