Restriction map for
pF1KB6854
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# NO CUT ENZYMES ( 149 enzymes) #
##################################
AatII Acc65I AccI AclI AfaI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AviII AvrII
BamHI BanI BbeI BbrPI BbvCI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsgI BsiEI BsiHKAI BsiWI
BsmFI Bsp1407I BspDI BspHI BspLU11I
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstEII BstPI
BstXI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI KasI
KpnI MaeI MamI MfeI MluI
MseI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NlaIII NotI
NruI NsiI NspI PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
PvuII RcaI RsaI RsrII SacI
SalI SapI SbfI ScaI SexAI
SfaNI SfoI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI Tru9I Tth111I
Van91I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccIII 110 TCCGGA T^CCGGA
AcsI 139 AAATTT [GA]^AATT[CT]
ApoI 139 AAATTT [GA]^AATT[CT]
BanII 106 GGGCTC G[GA]GC[CT]^C
BbsI 340 GTCTTC GAAGAC(2/6)
BbvI 231 GCTGC GCAGC(8/12)
BglI 503 GCCGCCCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 106 GGGCTC G[AGT]GC[ACT]^C
BpuAI 340 GTCTTC GAAGAC(2/6)
BsaI 51 GAGACC GGTCTC(1/5)
BsaWI 110 TCCGGA [AT]^CCGG[AT]
BseAI 110 TCCGGA T^CCGGA
BsmI 528 GAATGC GAATGC(1/-1)
Bsp1286I 106 GGGCTC G[AGT]GC[ACT]^C
BspEI 110 TCCGGA T^CCGGA
BspMI 417 GCAGGT ACCTGC(4/8)
BsrI 4 CCAGT ACTGG(1/-1)
BstBI 214 TTCGAA TT^CGAA
BstYI 155 AGATCC [GA]^GATC[CT]
DdeI 315 CTTAG C^T[ACGT]AG
DraII 197 AGGACCC [GA]G^G[ACGT]CC[CT]
EarI 519 GAAGAG CTCTTC(1/4)
EcoO109I 197 AGGACCC [GA]G^G[ACGT]CC[CT]
FauI 45 CCCGC CCCGC(4/6)
HaeII 395 AGCGCC [GA]GCGC^[CT]
HinfI 454 GATTC G^A[ACGT]TC
HphI 423 GGTGA GGTGA(8/7)
Ksp632I 519 GAAGAG CTCTTC(1/4)
KspI 46 CCGCGG CCGC^GG
MaeII 120 ACGT A^CGT
MaeIII 40 GTGAC ^GT[ACGT]AC
MflI 155 AGATCC [GA]^GATC[CT]
MroI 110 TCCGGA T^CCGGA
MslI 249 CATTGGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 46 CCGCGG C[AC]G^C[GT]G
NspV 214 TTCGAA TT^CGAA
PpuMI 197 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 333 GACCCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 328 CTGCAG CTGCA^G
SacII 46 CCGCGG CCGC^GG
SfcI 328 CTGCAG C^T[GA][CT]AG
SfiI 502 GGCCGCCCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 214 TTCGAA TT^CGAA
TfiI 454 GATTC G^A[AT]TC
TseI 231 GCTGC G^C[AT]GC
Tsp45I 40 GTGAC ^GT[GC]AC
Tsp509I 140 AATT ^AATT
TspRI 5 CAGTG CA[GC]TG[ACGT][ACGT]^
XcmI 543 CCAGGGTGGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 155 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AcyI 206 GGCGTC G[GA]^CG[CT]C
323 GACGCC
AlwI 156 GATCC GGATC(4/5)
387 GATCC
AvaI 170 CCCGGG C^[CT]CG[GA]G
507 CCCGGG
BalI 149 TGGCCA TGG^CCA
356 TGGCCA
BpmI 129 CTGGAG CTGGAG(16/14)
560 CTGGAG
BsaHI 206 GGCGTC G[GA]^CG[CT]C
323 GACGCC
BseRI 571 GAGGAG GAGGAG(10/8)
574 GAGGAG
BsoBI 170 CCCGGG C^[CT]CG[GA]G
507 CCCGGG
CfoI 396 GCGC GCG^C
483 GCGC
DsaI 46 CCGCGG C^C[GA][CT]GG
202 CCACGG
HhaI 396 GCGC GCG^C
483 GCGC
Hin1I 206 GGCGTC G[GA]^CG[CT]C
323 GACGCC
HinP1I 396 GCGC G^CGC
483 GCGC
MluNI 149 TGGCCA TGG^CCA
356 TGGCCA
MscI 149 TGGCCA TGG^CCA
356 TGGCCA
SmaI 170 CCCGGG CCC^GGG
507 CCCGGG
XmaCI 170 CCCGGG C^CCGGG
507 CCCGGG
XmaI 170 CCCGGG C^CCGGG
507 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AccII AciI AluI AvaII BcnI
BsaJI BsiYI BslI BsmAI BsmBI
BssKI BstF5I BstNI BstUI Cac8I
Cfr13I DpnII EaeI EcoRII Fnu4HI
FokI HaeIII HapII HgaI HpaII
ItaI MboI MboII MnlI MspI
MvaI MvnI MwoI NciI NdeII
NlaIV Sau3AI Sau96I ScrFI TaqI
TthHB8I