Restriction map for
pF1KB6415
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# NO CUT ENZYMES ( 121 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AflII AflIII AgeI AlwI AocI
ApoI AscI AseI AsnI Asp718I
AspI AvrII BamHI BbrPI BbsI
BbvCI BcgI BciVI BclI BfaI
BfrI BglII BlnI BpmI BpuAI
BsaAI BsaBI BsaWI BseAI BseRI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrGI Bst1107I
BstAPI BstBI BstEII BstPI BstYI
BstZ17I Bsu36I ClaI CpoI DraI
DraIII DrdI Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
HgaI HincII HindII HindIII HpaI
HphI KpnI MaeI MaeII MamI
MfeI MflI MluI MroI MseI
MunI NaeI NdeI NgoMIV NheI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SalI SapI ScaI
SfiI SfuI SgrAI SmlI SnaBI
SpeI SspBI SspI SwaI TfiI
Tru9I Tsp509I Tth111I XbaI XhoI
XhoII
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# ONE CUT ENZYMES ( 68 enzymes) #
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AccI 424 GTCTAC GT^[AC][GT]AC
AcyI 294 GGCGCC G[GA]^CG[CT]C
AfaI 504 GTAC GT^AC
AhdI 192 GACCCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 566 GTGCAC G^TGCAC
AlwNI 721 CAGGCGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 836 AGCGCT AGC^GCT
ApaI 87 GGGCCC GGGCC^C
ApaLI 566 GTGCAC G^TGCAC
Asp700I 834 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 192 GACCCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 566 GTGCAC G[AT]GC[AT]^C
AviII 758 TGCGCA TGC^GCA
BalI 476 TGGCCA TGG^CCA
BanII 87 GGGCCC G[GA]GC[CT]^C
BbeI 294 GGCGCC GGCGC^C
BlpI 237 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 237 GCTCAGC GC^T[ACGT]AGC
BsaHI 294 GGCGCC G[GA]^CG[CT]C
BsaI 636 GGTCTC GGTCTC(1/5)
BsgI 103 CTGCAC GTGCAG(16/14)
BsiHKAI 566 GTGCAC G[AT]GC[AT]^C
BspMI 137 ACCTGC ACCTGC(4/8)
BsrBI 26 GAGCGG CCGCTC(-3/-3)
BsrDI 704 CATTGC GCAATG(2/0)
BsrFI 120 GCCGGT [GA]^CCGG[CT]
BsrI 212 ACTGG ACTGG(1/-1)
BssHII 322 GCGCGC G^CGCGC
BstXI 389 CCATTGTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 237 GCTCAGC GC^T[ACGT]AGC
Cfr10I 120 GCCGGT [GA]^CCGG[CT]
DpnII 382 GATC ^GATC
Eam1105I 192 GACCCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 406 GAAGAG CTCTTC(1/4)
Eco47III 836 AGCGCT AGC^GCT
FspI 758 TGCGCA TGC^GCA
Hin1I 294 GGCGCC G[GA]^CG[CT]C
KasI 294 GGCGCC G^GCGCC
Ksp632I 406 GAAGAG CTCTTC(1/4)
MboI 382 GATC ^GATC
MluNI 476 TGGCCA TGG^CCA
MscI 476 TGGCCA TGG^CCA
NarI 294 GGCGCC GG^CGCC
NcoI 479 CCATGG C^CATGG
NdeII 382 GATC ^GATC
NotI 172 GCGGCCGC GC^GGCCGC
NspI 116 GCATGC [GA]CATG^[CT]
PflMI 468 CCACTGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 139 CTGCAG CTGCA^G
PvuII 556 CAGCTG CAG^CTG
RsaI 504 GTAC GT^AC
Sau3AI 382 GATC ^GATC
SbfI 138 CCTGCAGG CCTGCA^GG
SexAI 617 ACCTGGT A^CC[AT]GGT
SfcI 139 CTGCAG C^T[GA][CT]AG
SfoI 294 GGCGCC GGC^GCC
SmaI 156 CCCGGG CCC^GGG
SphI 116 GCATGC GCATG^C
Sse8387I 138 CCTGCAGG CCTGCA^GG
StuI 536 AGGCCT AGG^CCT
TaqI 530 TCGA T^CGA
Tsp45I 511 GTGAC ^GT[GC]AC
TthHB8I 530 TCGA T^CGA
Van91I 468 CCACTGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 590 CCATCATCCTCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 156 CCCGGG C^CCGGG
XmaI 156 CCCGGG C^CCGGG
XmnI 834 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 17 enzymes) #
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AvaI 156 CCCGGG C^[CT]CG[GA]G
217 CCCGAG
BglI 65 GCCGTCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
163 GCCCTGACGGC
BsiEI 173 CGGCCG CG[GA][CT]^CG
179 CGGCCG
BsmAI 691 GAGAC GTCTC(1/5)
637 GTCTC
BsoBI 156 CCCGGG C^[CT]CG[GA]G
217 CCCGAG
BssSI 598 CTCGTG CACGAG(-5/-1)
629 CTCGTG
EagI 173 CGGCCG C^GGCCG
179 CGGCCG
EclXI 173 CGGCCG C^GGCCG
179 CGGCCG
Eco52I 173 CGGCCG C^GGCCG
179 CGGCCG
EcoT14I 479 CCATGG C^C[AT][AT]GG
819 CCTTGG
KspI 39 CCGCGG CCGC^GG
176 CCGCGG
MaeIII 511 GTGAC ^GT[ACGT]AC
863 GTAAC
MboII 406 GAAGA GAAGA(8/7)
329 TCTTC
PpuMI 190 GGGACCC [GA]G^G[AT]CC[CT]
198 GGGTCCC
SacII 39 CCGCGG CCGC^GG
176 CCGCGG
StyI 479 CCATGG C^C[AT][AT]GG
819 CCTTGG
TspRI 469 CACTG CA[GC]TG[ACGT][ACGT]^
509 CAGTG
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# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AccII AciI AluI AvaII BanI
BbvI BcnI BmyI BsaJI BsiYI
BslI BsmFI Bsp1286I BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DraII DsaI EaeI EcoO109I
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII ItaI MnlI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NlaIII NlaIV PleI Sau96I ScrFI
SfaNI TseI