Restriction map for
pF1KB6753
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# NO CUT ENZYMES ( 201 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AflII AflIII
AgeI AhdI Alw44I AlwI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AvaI AviII AvrII BalI
BamHI BanI BanII BbeI BbrPI
BbvCI BcgI BciVI BclI BcnI
BfrI BglI BglII BlnI BlpI
BmyI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BseRI BsgI
BsiEI BsiHKAI BsiWI BsmBI BsmI
BsoBI Bsp1286I Bsp1407I BspDI BspEI
BspLU11I BspMI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstYI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DpnII DraI DraIII
DsaI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FauI FbaI FseI FspI HaeII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HpaI HpaII HphI
KasI KpnI Ksp632I KspI MaeII
MaeIII MamI MboI MfeI MflI
MluI MluNI MroI MscI MslI
MspA1I MspI MunI MvnI MwoI
NaeI NarI NciI NcoI NdeI
NdeII NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI PvuII RsaI RsrII SacI
SacII SalI SapI Sau3AI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI TfiI Tsp45I Tth111I Van91I
XbaI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AcsI 235 GAATTT [GA]^AATT[CT]
AlwNI 23 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 235 GAATTT [GA]^AATT[CT]
BbsI 58 GTCTTC GAAGAC(2/6)
BbvI 142 GCTGC GCAGC(8/12)
BpmI 12 CTCCAG CTGGAG(16/14)
BpuAI 58 GTCTTC GAAGAC(2/6)
BsaJI 168 CCCTGG C^C[ACGT][ACGT]GG
BsmAI 25 GAGAC GTCTC(1/5)
BspHI 125 TCATGA T^CATGA
BsrBI 31 GAGCGG CCGCTC(-3/-3)
BsrI 198 ACTGG ACTGG(1/-1)
BssKI 169 CCTGG ^CC[ACGT]GG
BstF5I 81 GGATG GGATG(2/0)
BstNI 169 CCTGG CC^[AT]GG
Cac8I 268 GCTTGC GC[ACGT]^[ACGT]GC
DraII 164 AGGACCC [GA]G^G[ACGT]CC[CT]
DrdI 166 GACCCTGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 164 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoRII 169 CCTGG ^CC[AT]GG
Fnu4HI 142 GCTGC GC^[ACGT]GC
FokI 81 GGATG GGATG(9/13)
HaeIII 256 GGCC GG^CC
HindIII 120 AAGCTT A^AGCTT
ItaI 142 GCTGC GC^[ACGT]GC
MnlI 285 CCTC CCTC(7/6)
MseI 242 TTAA T^TAA
MvaI 169 CCTGG CC^[AT]GG
NlaIV 165 GGACCC GG[ACGT]^[ACGT]CC
PpuMI 164 AGGACCC [GA]G^G[AT]CC[CT]
RcaI 125 TCATGA T^CATGA
ScrFI 169 CCTGG CC^[ACGT]GG
SfaNI 38 GCATC GCATC(5/9)
SfcI 20 CTACAG C^T[GA][CT]AG
SmlI 286 CTCAAG C^T[CT][GA]AG
TaqI 41 TCGA T^CGA
Tru9I 242 TTAA T^TAA
TseI 142 GCTGC G^C[AT]GC
Tsp509I 236 AATT ^AATT
TspRI 210 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 41 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 10 enzymes) #
###################################
AvaII 165 GGACC G^G[AT]CC
311 GGACC
BsiYI 258 CCTAGCTATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
302 CCCAGAAGCGG
BslI 258 CCTAGCTATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
302 CCCAGAAGCGG
BsmFI 45 GTCCC GGGAC(10/14)
295 GTCCC
Cfr13I 165 GGACC G^G[ACGT]CC
311 GGACC
HinfI 43 GAGTC G^A[ACGT]TC
277 GACTC
MboII 192 GAAGA GAAGA(8/7)
59 TCTTC
PleI 277 GACTC GAGTC(4/5)
43 GAGTC
Sau96I 165 GGACC G^G[ACGT]CC
311 GGACC
XcmI 63 CCAGAAGTATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
159 CCAGAAGGACCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 6 enzymes) #
#########################################
AciI AluI BfaI DdeI MaeI
NlaIII