Restriction map for
pF1KE0540
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# NO CUT ENZYMES ( 135 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbvCI BcgI BciVI BfrI BlnI
BsaAI BsaBI BsaHI BsaI BseAI
BseRI BsiHKAI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrGI BssHII BssSI Bst1107I BstEII
BstPI BstZ17I ClaI CpoI DraI
DraIII Eam1105I Eco47III EcoO65I EcoRI
EcoRV EcoT22I FseI FspI HaeII
HgaI Hin1I HincII HindII HindIII
HpaI HphI KasI KpnI KspI
MamI MfeI MluI MluNI MroI
MscI MseI MunI NarI NdeI
NheI NotI NruI NsiI NspI
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfoI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TfiI Tru9I Tsp509I Tth111I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AccII 616 CGCG CG^CG
AlwI 321 GGATC GGATC(4/5)
AocI 231 CCTGAGG CC^T[ACGT]AGG
AvaI 505 CCCGAG C^[CT]CG[GA]G
BbsI 487 GTCTTC GAAGAC(2/6)
BclI 185 TGATCA T^GATCA
BfaI 602 CTAG C^TAG
BglII 746 AGATCT A^GATCT
BlpI 739 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 739 GCTGAGC GC^T[ACGT]AGC
BpuAI 487 GTCTTC GAAGAC(2/6)
BsaWI 57 TCCGGT [AT]^CCGG[AT]
BsiEI 723 CGGCCG CG[GA][CT]^CG
BsmAI 133 GAGAC GTCTC(1/5)
BsoBI 505 CCCGAG C^[CT]CG[GA]G
BsrBI 508 GAGCGG CCGCTC(-3/-3)
BsrDI 675 CATTGC GCAATG(2/0)
BstAPI 435 GCAGGTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 170 TTCGAA TT^CGAA
BstF5I 642 GGATG GGATG(2/0)
BstUI 616 CGCG CG^CG
BstXI 156 CCAGGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 746 AGATCT [GA]^GATC[CT]
Bsu36I 231 CCTGAGG CC^T[ACGT]AGG
CelII 739 GCTGAGC GC^T[ACGT]AGC
CfoI 282 GCGC GCG^C
DrdI 560 GACTGTTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 527 CCATGG C^C[GA][CT]GG
EaeI 723 CGGCCG [CT]^GGCC[GA]
EagI 723 CGGCCG C^GGCCG
EarI 706 CTCTTC CTCTTC(1/4)
EclXI 723 CGGCCG C^GGCCG
Eco52I 723 CGGCCG C^GGCCG
Eco81I 231 CCTGAGG CC^T[ACGT]AGG
EcoNI 279 CCTGCGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 185 TGATCA T^GATCA
FokI 642 GGATG GGATG(9/13)
HhaI 282 GCGC GCG^C
HinP1I 282 GCGC G^CGC
Ksp632I 706 CTCTTC CTCTTC(1/4)
MaeI 602 CTAG C^TAG
MflI 746 AGATCT [GA]^GATC[CT]
MslI 492 CATCACTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 616 CGCG CG^CG
NaeI 631 GCCGGC GCC^GGC
NcoI 527 CCATGG C^CATGG
NgoMIV 631 GCCGGC G^CCGGC
NspV 170 TTCGAA TT^CGAA
SfaNI 93 GCATC GCATC(5/9)
SfiI 219 GGCCTGGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 170 TTCGAA TT^CGAA
SmlI 49 CTCAAG C^T[CT][GA]AG
StuI 192 AGGCCT AGG^CCT
TaqI 171 TCGA T^CGA
Tsp45I 605 GTCAC ^GT[GC]AC
TthHB8I 171 TCGA T^CGA
XhoII 746 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AfaI 378 GTAC GT^AC
621 GTAC
AlwNI 85 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
458 CAGAGCCTG
ApaI 123 GGGCCC GGGCC^C
159 GGGCCC
BglI 220 GCCTGGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
229 GCCCTGAGGGC
BpmI 473 CTCCAG CTGGAG(16/14)
421 CTGGAG
BsgI 433 GTGCAG GTGCAG(16/14)
478 GTGCAG
BsmFI 70 GGGAC GGGAC(10/14)
512 GGGAC
BsrFI 574 ACCGGC [GA]^CCGG[CT]
631 GCCGGC
BsrI 363 CCAGT ACTGG(1/-1)
475 CCAGT
Cfr10I 574 ACCGGC [GA]^CCGG[CT]
631 GCCGGC
EcoT14I 527 CCATGG C^C[AT][AT]GG
638 CCAAGG
FauI 533 CCCGC CCCGC(4/6)
510 GCGGG
HinfI 12 GACTC G^A[ACGT]TC
209 GACTC
MaeII 518 ACGT A^CGT
539 ACGT
MaeIII 605 GTCAC ^GT[ACGT]AC
611 GTTAC
MboII 488 TCTTC GAAGA(8/7)
707 TCTTC
MspA1I 290 CAGCTG C[AC]G^C[GT]G
448 CAGCTG
NlaIII 528 CATG CATG^
771 CATG
PflMI 23 CCACAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
312 CCACCTGCTGG
PleI 12 GACTC GAGTC(4/5)
209 GACTC
PpuMI 70 GGGACCC [GA]G^G[AT]CC[CT]
100 AGGTCCT
PstI 128 CTGCAG CTGCA^G
253 CTGCAG
PvuII 290 CAGCTG CAG^CTG
448 CAGCTG
RsaI 378 GTAC GT^AC
621 GTAC
SfcI 128 CTGCAG C^T[GA][CT]AG
253 CTGCAG
StyI 527 CCATGG C^C[AT][AT]GG
638 CCAAGG
Van91I 23 CCACAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
312 CCACCTGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AciI AluI AvaII BanII BbvI
BcnI BmyI BsaJI BsiYI BslI
Bsp1286I BspMI BssKI BstNI Cac8I
Cfr13I DdeI DpnII DraII EcoO109I
EcoRII Fnu4HI HaeIII HapII HpaII
ItaI MboI MnlI MspI MvaI
MwoI NciI NdeII NlaIV Sau3AI
Sau96I ScrFI TseI TspRI