Restriction map for
pF1KB6874
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# NO CUT ENZYMES ( 164 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AviII AvrII
BalI BamHI BbeI BbrPI BbvCI
BcgI BciVI BclI BfrI BglI
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsiEI BsiHKAI BsiWI
BsmBI BsmI Bsp1407I BspDI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BsrI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I Bsu36I Cac8I CelII Cfr10I
ClaI CpoI DraI DraIII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HaeII HgaI Hin1I HincII
HindII HindIII HpaI KasI KpnI
KspI MaeII MaeIII MamI MfeI
MluI MluNI MscI MseI MslI
MspA1I MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tru9I Tsp45I Tsp509I
Tth111I XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AccIII 115 TCCGGA T^CCGGA
AfaI 507 GTAC GT^AC
AlwNI 278 CAGGGTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 148 GGGCCC GGGCC^C
AvaI 151 CCCGGG C^[CT]CG[GA]G
BanI 246 GGTGCC G^G[CT][GA]CC
BglII 192 AGATCT A^GATCT
BpmI 188 CTGGAG CTGGAG(16/14)
BsaWI 115 TCCGGA [AT]^CCGG[AT]
BseAI 115 TCCGGA T^CCGGA
BseRI 319 GAGGAG GAGGAG(10/8)
BsgI 403 GTGCAG GTGCAG(16/14)
BsmAI 67 GAGAC GTCTC(1/5)
BsmFI 295 GTCCC GGGAC(10/14)
BsoBI 151 CCCGGG C^[CT]CG[GA]G
BspEI 115 TCCGGA T^CCGGA
BstYI 192 AGATCT [GA]^GATC[CT]
CfoI 22 GCGC GCG^C
EcoT14I 242 CCTTGG C^C[AT][AT]GG
FauI 7 CCCGC CCCGC(4/6)
HhaI 22 GCGC GCG^C
HinP1I 22 GCGC G^CGC
HphI 349 TCACC GGTGA(8/7)
MflI 192 AGATCT [GA]^GATC[CT]
MroI 115 TCCGGA T^CCGGA
PflMI 237 CCATCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 69 GACTGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsaI 507 GTAC GT^AC
SapI 33 GCTCTTC GCTCTTC(1/4)
SmaI 151 CCCGGG CCC^GGG
StyI 242 CCTTGG C^C[AT][AT]GG
TspRI 399 CACTG CA[GC]TG[ACGT][ACGT]^
Van91I 237 CCATCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 252 TCTAGA T^CTAGA
XhoII 192 AGATCT [GA]^GATC[CT]
XmaCI 151 CCCGGG C^CCGGG
XmaI 151 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccII 21 CGCG CG^CG
591 CGCG
AciI 8 CCGC CCGC(-3/-1)
44 GCGG
AlwI 60 GATCC GGATC(4/5)
492 GATCC
AvaII 90 GGACC G^G[AT]CC
435 GGTCC
BanII 119 GAGCCC G[GA]GC[CT]^C
148 GGGCCC
BbsI 208 GAAGAC GAAGAC(2/6)
484 GTCTTC
BcnI 151 CCCGG CC^[GC]GG
152 CCGGG
BmyI 119 GAGCCC G[AGT]GC[ACT]^C
148 GGGCCC
BpuAI 208 GAAGAC GAAGAC(2/6)
484 GTCTTC
BsiYI 237 CCATCCCTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
267 CCCTGAGAGGG
BslI 237 CCATCCCTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
267 CCCTGAGAGGG
Bsp1286I 119 GAGCCC G[AGT]GC[ACT]^C
148 GGGCCC
BstUI 21 CGCG CG^CG
591 CGCG
DdeI 71 CTGAG C^T[ACGT]AG
269 CTGAG
EarI 34 CTCTTC CTCTTC(1/4)
358 GAAGAG
HapII 116 CCGG C^CGG
152 CCGG
HpaII 116 CCGG C^CGG
152 CCGG
Ksp632I 34 CTCTTC CTCTTC(1/4)
358 GAAGAG
MspI 116 CCGG C^CGG
152 CCGG
MvnI 21 CGCG CG^CG
591 CGCG
MwoI 24 GCTGCTGCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
461 GCATCGTGGGC
NciI 151 CCCGG CC^[GC]GG
152 CCGGG
NlaIII 216 CATG CATG^
504 CATG
PpuMI 89 AGGACCT [GA]G^G[AT]CC[CT]
434 AGGTCCT
SfaNI 461 GCATC GCATC(5/9)
470 GCATC
TaqI 58 TCGA T^CGA
80 TCGA
TfiI 160 GAATC G^A[AT]TC
344 GAATC
TthHB8I 58 TCGA T^CGA
80 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 29 enzymes) #
#########################################
AluI BbvI BfaI BsaJI BssKI
BstF5I BstNI Cfr13I DpnII DraII
EcoO109I EcoRII Fnu4HI FokI HaeIII
HinfI ItaI MaeI MboI MboII
MnlI MvaI NdeII NlaIV PleI
Sau3AI Sau96I ScrFI TseI