Restriction map for
pF1KE0541
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# NO CUT ENZYMES ( 155 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AfaI AflII AflIII AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AviII AvrII BalI BbeI BbrPI
BbvCI BcgI BciVI BclI BfrI
BglI BglII BlnI BlpI BpmI
Bpu1102I BsaBI BsaI BseAI BseRI
BsiEI BsiWI BsmAI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrGI BssHII
Bst1107I BstAPI BstBI BstXI BstZ17I
Bsu36I CelII ClaI CpoI DdeI
DraI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoRI
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HindIII HinfI HpaI
KasI KpnI Ksp632I MamI MfeI
MluI MluNI MroI MscI MseI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PleI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsaI RsrII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI Sse8387I SspBI
SspI StuI SwaI TaqI TfiI
Tru9I Tsp509I Tth111I TthHB8I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AgeI 91 ACCGGT A^CCGGT
AspHI 100 GAGCTC G[AT]GC[AT]^C
BbsI 403 GAAGAC GAAGAC(2/6)
BfaI 629 CTAG C^TAG
BpuAI 403 GAAGAC GAAGAC(2/6)
BsaAI 594 TACGTG [CT]AC^GT[GA]
BsaWI 91 ACCGGT [AT]^CCGG[AT]
BsiHKAI 100 GAGCTC G[AT]GC[AT]^C
BsmFI 285 GTCCC GGGAC(10/14)
BssSI 103 CTCGTG CACGAG(-5/-1)
BstEII 204 GGTGACC G^GT[ACGT]ACC
BstF5I 543 CATCC GGATG(2/0)
BstPI 204 GGTGACC G^GT[ACGT]ACC
DraII 283 GGGTCCC [GA]G^G[ACGT]CC[CT]
DraIII 145 CACCGCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 108 GACCTGCGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 283 GGGTCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 204 GGTGACC G^GT[ACGT]ACC
EcoT14I 605 CCATGG C^C[AT][AT]GG
FokI 543 CATCC GGATG(9/13)
HaeII 57 GGCGCT [GA]GCGC^[CT]
MaeI 629 CTAG C^TAG
MaeII 595 ACGT A^CGT
NcoI 605 CCATGG C^CATGG
PflMI 486 CCAGCATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 91 ACCGGT A^CCGGT
PpuMI 283 GGGTCCC [GA]G^G[AT]CC[CT]
PstI 644 CTGCAG CTGCA^G
SacI 100 GAGCTC GAGCT^C
SfaNI 614 GCATC GCATC(5/9)
SfcI 644 CTGCAG C^T[GA][CT]AG
SphI 569 GCATGC GCATG^C
StyI 605 CCATGG C^C[AT][AT]GG
Van91I 486 CCAGCATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AcyI 219 GGCGTC G[GA]^CG[CT]C
229 GACGCC
BamHI 181 GGATCC G^GATCC
248 GGATCC
BanII 100 GAGCTC G[GA]GC[CT]^C
637 GAGCCC
BcnI 70 CCGGG CC^[GC]GG
288 CCCGG
BsaHI 219 GGCGTC G[GA]^CG[CT]C
229 GACGCC
BsgI 157 CTGCAC GTGCAG(16/14)
343 CTGCAC
BstYI 181 GGATCC [GA]^GATC[CT]
248 GGATCC
DpnII 182 GATC ^GATC
249 GATC
EaeI 31 TGGCCG [CT]^GGCC[GA]
192 CGGCCA
HgaI 229 GACGC GACGC(5/10)
220 GCGTC
Hin1I 219 GGCGTC G[GA]^CG[CT]C
229 GACGCC
KspI 15 CCGCGG CCGC^GG
252 CCGCGG
MboI 182 GATC ^GATC
249 GATC
MboII 403 GAAGA GAAGA(8/7)
617 TCTTC
MflI 181 GGATCC [GA]^GATC[CT]
248 GGATCC
MslI 318 CACGCATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
498 CACCTCTGTG
NciI 70 CCGGG CC^[GC]GG
288 CCCGG
NdeII 182 GATC ^GATC
249 GATC
SacII 15 CCGCGG CCGC^GG
252 CCGCGG
Sau3AI 182 GATC ^GATC
249 GATC
XhoII 181 GGATCC [GA]^GATC[CT]
248 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AccII AciI AluI AlwI AvaII
BanI BbvI BmyI BsaJI BsiYI
BslI Bsp1286I BspMI BsrFI BsrI
BssKI BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DsaI EcoRII FauI
Fnu4HI HaeIII HapII HhaI HinP1I
HpaII HphI ItaI MaeIII MnlI
MspA1I MspI MvaI MvnI MwoI
NlaIII NlaIV NspI Sau96I ScrFI
TseI Tsp45I TspRI